Job ID = 16439651 SRX = SRX11499353 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18411413 spots for SRR15192696/SRR15192696.sra Written 18411413 spots for SRR15192696/SRR15192696.sra Read 14064560 spots for SRR15192697/SRR15192697.sra Written 14064560 spots for SRR15192697/SRR15192697.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440600 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:43:35 32475973 reads; of these: 32475973 (100.00%) were paired; of these: 4166163 (12.83%) aligned concordantly 0 times 21318911 (65.65%) aligned concordantly exactly 1 time 6990899 (21.53%) aligned concordantly >1 times ---- 4166163 pairs aligned concordantly 0 times; of these: 1698019 (40.76%) aligned discordantly 1 time ---- 2468144 pairs aligned 0 times concordantly or discordantly; of these: 4936288 mates make up the pairs; of these: 2674687 (54.18%) aligned 0 times 971954 (19.69%) aligned exactly 1 time 1289647 (26.13%) aligned >1 times 95.88% overall alignment rate Time searching: 01:43:36 Overall time: 01:43:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6498609 / 26278278 = 0.2473 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:01:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:01:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:01:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:01:09: 1000000 INFO @ Tue, 02 Aug 2022 18:01:15: 2000000 INFO @ Tue, 02 Aug 2022 18:01:22: 3000000 INFO @ Tue, 02 Aug 2022 18:01:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:01:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:01:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:01:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:01:36: 5000000 INFO @ Tue, 02 Aug 2022 18:01:39: 1000000 INFO @ Tue, 02 Aug 2022 18:01:43: 6000000 INFO @ Tue, 02 Aug 2022 18:01:45: 2000000 INFO @ Tue, 02 Aug 2022 18:01:50: 7000000 INFO @ Tue, 02 Aug 2022 18:01:52: 3000000 INFO @ Tue, 02 Aug 2022 18:01:57: 8000000 INFO @ Tue, 02 Aug 2022 18:01:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:02:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:02:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:02:03: 9000000 INFO @ Tue, 02 Aug 2022 18:02:05: 5000000 INFO @ Tue, 02 Aug 2022 18:02:09: 1000000 INFO @ Tue, 02 Aug 2022 18:02:11: 10000000 INFO @ Tue, 02 Aug 2022 18:02:12: 6000000 INFO @ Tue, 02 Aug 2022 18:02:16: 2000000 INFO @ Tue, 02 Aug 2022 18:02:18: 11000000 INFO @ Tue, 02 Aug 2022 18:02:19: 7000000 INFO @ Tue, 02 Aug 2022 18:02:22: 3000000 INFO @ Tue, 02 Aug 2022 18:02:25: 12000000 INFO @ Tue, 02 Aug 2022 18:02:25: 8000000 INFO @ Tue, 02 Aug 2022 18:02:29: 4000000 INFO @ Tue, 02 Aug 2022 18:02:32: 9000000 INFO @ Tue, 02 Aug 2022 18:02:32: 13000000 INFO @ Tue, 02 Aug 2022 18:02:36: 5000000 INFO @ Tue, 02 Aug 2022 18:02:39: 14000000 INFO @ Tue, 02 Aug 2022 18:02:39: 10000000 INFO @ Tue, 02 Aug 2022 18:02:42: 6000000 INFO @ Tue, 02 Aug 2022 18:02:46: 15000000 INFO @ Tue, 02 Aug 2022 18:02:47: 11000000 INFO @ Tue, 02 Aug 2022 18:02:49: 7000000 INFO @ Tue, 02 Aug 2022 18:02:53: 16000000 INFO @ Tue, 02 Aug 2022 18:02:53: 12000000 INFO @ Tue, 02 Aug 2022 18:02:56: 8000000 INFO @ Tue, 02 Aug 2022 18:03:00: 13000000 INFO @ Tue, 02 Aug 2022 18:03:00: 17000000 INFO @ Tue, 02 Aug 2022 18:03:02: 9000000 INFO @ Tue, 02 Aug 2022 18:03:07: 14000000 INFO @ Tue, 02 Aug 2022 18:03:07: 18000000 INFO @ Tue, 02 Aug 2022 18:03:10: 10000000 INFO @ Tue, 02 Aug 2022 18:03:13: 15000000 INFO @ Tue, 02 Aug 2022 18:03:14: 19000000 INFO @ Tue, 02 Aug 2022 18:03:17: 11000000 INFO @ Tue, 02 Aug 2022 18:03:20: 16000000 INFO @ Tue, 02 Aug 2022 18:03:21: 20000000 INFO @ Tue, 02 Aug 2022 18:03:23: 12000000 INFO @ Tue, 02 Aug 2022 18:03:27: 17000000 INFO @ Tue, 02 Aug 2022 18:03:28: 21000000 INFO @ Tue, 02 Aug 2022 18:03:30: 13000000 INFO @ Tue, 02 Aug 2022 18:03:34: 18000000 INFO @ Tue, 02 Aug 2022 18:03:35: 22000000 INFO @ Tue, 02 Aug 2022 18:03:37: 14000000 INFO @ Tue, 02 Aug 2022 18:03:41: 19000000 INFO @ Tue, 02 Aug 2022 18:03:42: 23000000 INFO @ Tue, 02 Aug 2022 18:03:44: 15000000 INFO @ Tue, 02 Aug 2022 18:03:48: 20000000 INFO @ Tue, 02 Aug 2022 18:03:49: 24000000 INFO @ Tue, 02 Aug 2022 18:03:50: 16000000 INFO @ Tue, 02 Aug 2022 18:03:54: 21000000 INFO @ Tue, 02 Aug 2022 18:03:56: 25000000 INFO @ Tue, 02 Aug 2022 18:03:57: 17000000 INFO @ Tue, 02 Aug 2022 18:04:01: 22000000 INFO @ Tue, 02 Aug 2022 18:04:03: 26000000 INFO @ Tue, 02 Aug 2022 18:04:04: 18000000 INFO @ Tue, 02 Aug 2022 18:04:08: 23000000 INFO @ Tue, 02 Aug 2022 18:04:10: 27000000 INFO @ Tue, 02 Aug 2022 18:04:11: 19000000 INFO @ Tue, 02 Aug 2022 18:04:15: 24000000 INFO @ Tue, 02 Aug 2022 18:04:17: 28000000 INFO @ Tue, 02 Aug 2022 18:04:18: 20000000 INFO @ Tue, 02 Aug 2022 18:04:22: 25000000 INFO @ Tue, 02 Aug 2022 18:04:24: 29000000 INFO @ Tue, 02 Aug 2022 18:04:25: 21000000 INFO @ Tue, 02 Aug 2022 18:04:29: 26000000 INFO @ Tue, 02 Aug 2022 18:04:31: 30000000 INFO @ Tue, 02 Aug 2022 18:04:32: 22000000 INFO @ Tue, 02 Aug 2022 18:04:36: 27000000 INFO @ Tue, 02 Aug 2022 18:04:38: 31000000 INFO @ Tue, 02 Aug 2022 18:04:38: 23000000 INFO @ Tue, 02 Aug 2022 18:04:43: 28000000 INFO @ Tue, 02 Aug 2022 18:04:45: 32000000 INFO @ Tue, 02 Aug 2022 18:04:45: 24000000 INFO @ Tue, 02 Aug 2022 18:04:49: 29000000 INFO @ Tue, 02 Aug 2022 18:04:51: 33000000 INFO @ Tue, 02 Aug 2022 18:04:52: 25000000 INFO @ Tue, 02 Aug 2022 18:04:56: 30000000 INFO @ Tue, 02 Aug 2022 18:04:58: 34000000 INFO @ Tue, 02 Aug 2022 18:04:59: 26000000 INFO @ Tue, 02 Aug 2022 18:05:03: 31000000 INFO @ Tue, 02 Aug 2022 18:05:05: 27000000 INFO @ Tue, 02 Aug 2022 18:05:06: 35000000 INFO @ Tue, 02 Aug 2022 18:05:10: 32000000 INFO @ Tue, 02 Aug 2022 18:05:12: 28000000 INFO @ Tue, 02 Aug 2022 18:05:13: 36000000 INFO @ Tue, 02 Aug 2022 18:05:17: 33000000 INFO @ Tue, 02 Aug 2022 18:05:19: 29000000 INFO @ Tue, 02 Aug 2022 18:05:20: 37000000 INFO @ Tue, 02 Aug 2022 18:05:24: 34000000 INFO @ Tue, 02 Aug 2022 18:05:25: 30000000 INFO @ Tue, 02 Aug 2022 18:05:27: 38000000 INFO @ Tue, 02 Aug 2022 18:05:30: 35000000 INFO @ Tue, 02 Aug 2022 18:05:32: 31000000 INFO @ Tue, 02 Aug 2022 18:05:34: 39000000 INFO @ Tue, 02 Aug 2022 18:05:37: 36000000 INFO @ Tue, 02 Aug 2022 18:05:39: 32000000 INFO @ Tue, 02 Aug 2022 18:05:40: 40000000 INFO @ Tue, 02 Aug 2022 18:05:44: 37000000 INFO @ Tue, 02 Aug 2022 18:05:45: 33000000 INFO @ Tue, 02 Aug 2022 18:05:47: 41000000 INFO @ Tue, 02 Aug 2022 18:05:51: 38000000 INFO @ Tue, 02 Aug 2022 18:05:52: 34000000 INFO @ Tue, 02 Aug 2022 18:05:54: 42000000 INFO @ Tue, 02 Aug 2022 18:05:58: 39000000 INFO @ Tue, 02 Aug 2022 18:05:59: 35000000 INFO @ Tue, 02 Aug 2022 18:06:01: 43000000 INFO @ Tue, 02 Aug 2022 18:06:05: 40000000 INFO @ Tue, 02 Aug 2022 18:06:05: 36000000 INFO @ Tue, 02 Aug 2022 18:06:08: 44000000 INFO @ Tue, 02 Aug 2022 18:06:12: 41000000 INFO @ Tue, 02 Aug 2022 18:06:12: 37000000 INFO @ Tue, 02 Aug 2022 18:06:15: 45000000 INFO @ Tue, 02 Aug 2022 18:06:19: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:06:19: 38000000 INFO @ Tue, 02 Aug 2022 18:06:22: 46000000 INFO @ Tue, 02 Aug 2022 18:06:25: 39000000 INFO @ Tue, 02 Aug 2022 18:06:25: 43000000 INFO @ Tue, 02 Aug 2022 18:06:29: 47000000 INFO @ Tue, 02 Aug 2022 18:06:32: 40000000 INFO @ Tue, 02 Aug 2022 18:06:32: 44000000 INFO @ Tue, 02 Aug 2022 18:06:37: 48000000 INFO @ Tue, 02 Aug 2022 18:06:39: 41000000 INFO @ Tue, 02 Aug 2022 18:06:39: 45000000 INFO @ Tue, 02 Aug 2022 18:06:44: 49000000 INFO @ Tue, 02 Aug 2022 18:06:46: 42000000 INFO @ Tue, 02 Aug 2022 18:06:46: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 18:06:46: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 18:06:46: #1 total tags in treatment: 22050211 INFO @ Tue, 02 Aug 2022 18:06:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:06:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:06:46: #1 tags after filtering in treatment: 20137072 INFO @ Tue, 02 Aug 2022 18:06:46: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:06:46: #1 finished! INFO @ Tue, 02 Aug 2022 18:06:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:06:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:06:46: 46000000 INFO @ Tue, 02 Aug 2022 18:06:47: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 18:06:47: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:06:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:06:52: 43000000 INFO @ Tue, 02 Aug 2022 18:06:53: 47000000 INFO @ Tue, 02 Aug 2022 18:06:59: 44000000 INFO @ Tue, 02 Aug 2022 18:07:00: 48000000 INFO @ Tue, 02 Aug 2022 18:07:06: 45000000 INFO @ Tue, 02 Aug 2022 18:07:07: 49000000 INFO @ Tue, 02 Aug 2022 18:07:09: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 18:07:09: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 18:07:09: #1 total tags in treatment: 22050211 INFO @ Tue, 02 Aug 2022 18:07:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:07:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:07:09: #1 tags after filtering in treatment: 20137072 INFO @ Tue, 02 Aug 2022 18:07:09: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:07:09: #1 finished! INFO @ Tue, 02 Aug 2022 18:07:09: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:07:11: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 18:07:11: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:07:12: 46000000 INFO @ Tue, 02 Aug 2022 18:07:19: 47000000 INFO @ Tue, 02 Aug 2022 18:07:26: 48000000 INFO @ Tue, 02 Aug 2022 18:07:32: 49000000 INFO @ Tue, 02 Aug 2022 18:07:34: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 18:07:34: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 18:07:34: #1 total tags in treatment: 22050211 INFO @ Tue, 02 Aug 2022 18:07:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:07:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:07:35: #1 tags after filtering in treatment: 20137072 INFO @ Tue, 02 Aug 2022 18:07:35: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:07:35: #1 finished! INFO @ Tue, 02 Aug 2022 18:07:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:07:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:07:36: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 18:07:36: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:07:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499353/SRX11499353.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。