Job ID = 16439650 SRX = SRX11499352 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20231606 spots for SRR15192694/SRR15192694.sra Written 20231606 spots for SRR15192694/SRR15192694.sra Read 16588744 spots for SRR15192695/SRR15192695.sra Written 16588744 spots for SRR15192695/SRR15192695.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441499 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:15:51 36820350 reads; of these: 36820350 (100.00%) were paired; of these: 4154062 (11.28%) aligned concordantly 0 times 24200176 (65.73%) aligned concordantly exactly 1 time 8466112 (22.99%) aligned concordantly >1 times ---- 4154062 pairs aligned concordantly 0 times; of these: 1680512 (40.45%) aligned discordantly 1 time ---- 2473550 pairs aligned 0 times concordantly or discordantly; of these: 4947100 mates make up the pairs; of these: 2660768 (53.78%) aligned 0 times 1000537 (20.22%) aligned exactly 1 time 1285795 (25.99%) aligned >1 times 96.39% overall alignment rate Time searching: 03:15:51 Overall time: 03:15:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7016944 / 29562577 = 0.2374 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:57:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:57:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:57:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:57:18: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:57:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:57:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:57:34: 2000000 INFO @ Tue, 02 Aug 2022 19:57:46: 1000000 INFO @ Tue, 02 Aug 2022 19:57:50: 3000000 INFO @ Tue, 02 Aug 2022 19:57:58: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:58:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:58:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:58:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:58:06: 4000000 INFO @ Tue, 02 Aug 2022 19:58:10: 3000000 INFO @ Tue, 02 Aug 2022 19:58:20: 1000000 INFO @ Tue, 02 Aug 2022 19:58:22: 4000000 INFO @ Tue, 02 Aug 2022 19:58:23: 5000000 INFO @ Tue, 02 Aug 2022 19:58:34: 5000000 INFO @ Tue, 02 Aug 2022 19:58:36: 2000000 INFO @ Tue, 02 Aug 2022 19:58:39: 6000000 INFO @ Tue, 02 Aug 2022 19:58:47: 6000000 INFO @ Tue, 02 Aug 2022 19:58:54: 3000000 INFO @ Tue, 02 Aug 2022 19:58:54: 7000000 INFO @ Tue, 02 Aug 2022 19:59:01: 7000000 INFO @ Tue, 02 Aug 2022 19:59:11: 8000000 INFO @ Tue, 02 Aug 2022 19:59:12: 4000000 INFO @ Tue, 02 Aug 2022 19:59:17: 8000000 INFO @ Tue, 02 Aug 2022 19:59:28: 9000000 INFO @ Tue, 02 Aug 2022 19:59:30: 5000000 INFO @ Tue, 02 Aug 2022 19:59:32: 9000000 INFO @ Tue, 02 Aug 2022 19:59:44: 10000000 INFO @ Tue, 02 Aug 2022 19:59:47: 10000000 INFO @ Tue, 02 Aug 2022 19:59:48: 6000000 INFO @ Tue, 02 Aug 2022 20:00:00: 11000000 INFO @ Tue, 02 Aug 2022 20:00:00: 11000000 INFO @ Tue, 02 Aug 2022 20:00:04: 7000000 INFO @ Tue, 02 Aug 2022 20:00:12: 12000000 INFO @ Tue, 02 Aug 2022 20:00:16: 12000000 INFO @ Tue, 02 Aug 2022 20:00:20: 8000000 INFO @ Tue, 02 Aug 2022 20:00:25: 13000000 INFO @ Tue, 02 Aug 2022 20:00:33: 13000000 INFO @ Tue, 02 Aug 2022 20:00:37: 9000000 INFO @ Tue, 02 Aug 2022 20:00:38: 14000000 INFO @ Tue, 02 Aug 2022 20:00:49: 14000000 INFO @ Tue, 02 Aug 2022 20:00:51: 15000000 INFO @ Tue, 02 Aug 2022 20:00:53: 10000000 INFO @ Tue, 02 Aug 2022 20:01:02: 16000000 INFO @ Tue, 02 Aug 2022 20:01:05: 15000000 INFO @ Tue, 02 Aug 2022 20:01:09: 11000000 INFO @ Tue, 02 Aug 2022 20:01:13: 17000000 INFO @ Tue, 02 Aug 2022 20:01:21: 16000000 INFO @ Tue, 02 Aug 2022 20:01:25: 12000000 INFO @ Tue, 02 Aug 2022 20:01:26: 18000000 INFO @ Tue, 02 Aug 2022 20:01:37: 17000000 INFO @ Tue, 02 Aug 2022 20:01:38: 19000000 INFO @ Tue, 02 Aug 2022 20:01:42: 13000000 INFO @ Tue, 02 Aug 2022 20:01:50: 20000000 INFO @ Tue, 02 Aug 2022 20:01:53: 18000000 INFO @ Tue, 02 Aug 2022 20:01:58: 14000000 INFO @ Tue, 02 Aug 2022 20:02:01: 21000000 INFO @ Tue, 02 Aug 2022 20:02:08: 19000000 INFO @ Tue, 02 Aug 2022 20:02:12: 22000000 INFO @ Tue, 02 Aug 2022 20:02:13: 15000000 INFO @ Tue, 02 Aug 2022 20:02:23: 20000000 INFO @ Tue, 02 Aug 2022 20:02:25: 23000000 INFO @ Tue, 02 Aug 2022 20:02:30: 16000000 INFO @ Tue, 02 Aug 2022 20:02:37: 24000000 INFO @ Tue, 02 Aug 2022 20:02:38: 21000000 INFO @ Tue, 02 Aug 2022 20:02:47: 17000000 INFO @ Tue, 02 Aug 2022 20:02:48: 25000000 INFO @ Tue, 02 Aug 2022 20:02:53: 22000000 INFO @ Tue, 02 Aug 2022 20:03:01: 26000000 INFO @ Tue, 02 Aug 2022 20:03:04: 18000000 INFO @ Tue, 02 Aug 2022 20:03:09: 23000000 INFO @ Tue, 02 Aug 2022 20:03:15: 27000000 INFO @ Tue, 02 Aug 2022 20:03:20: 19000000 INFO @ Tue, 02 Aug 2022 20:03:26: 24000000 INFO @ Tue, 02 Aug 2022 20:03:27: 28000000 INFO @ Tue, 02 Aug 2022 20:03:37: 20000000 INFO @ Tue, 02 Aug 2022 20:03:40: 29000000 INFO @ Tue, 02 Aug 2022 20:03:43: 25000000 INFO @ Tue, 02 Aug 2022 20:03:52: 30000000 INFO @ Tue, 02 Aug 2022 20:03:53: 21000000 INFO @ Tue, 02 Aug 2022 20:04:00: 26000000 INFO @ Tue, 02 Aug 2022 20:04:04: 31000000 INFO @ Tue, 02 Aug 2022 20:04:09: 22000000 INFO @ Tue, 02 Aug 2022 20:04:15: 32000000 INFO @ Tue, 02 Aug 2022 20:04:16: 27000000 INFO @ Tue, 02 Aug 2022 20:04:25: 23000000 INFO @ Tue, 02 Aug 2022 20:04:26: 33000000 INFO @ Tue, 02 Aug 2022 20:04:32: 28000000 INFO @ Tue, 02 Aug 2022 20:04:39: 34000000 INFO @ Tue, 02 Aug 2022 20:04:42: 24000000 INFO @ Tue, 02 Aug 2022 20:04:48: 29000000 INFO @ Tue, 02 Aug 2022 20:04:50: 35000000 INFO @ Tue, 02 Aug 2022 20:04:59: 25000000 INFO @ Tue, 02 Aug 2022 20:05:00: 36000000 INFO @ Tue, 02 Aug 2022 20:05:04: 30000000 INFO @ Tue, 02 Aug 2022 20:05:11: 37000000 INFO @ Tue, 02 Aug 2022 20:05:17: 26000000 INFO @ Tue, 02 Aug 2022 20:05:20: 31000000 INFO @ Tue, 02 Aug 2022 20:05:23: 38000000 INFO @ Tue, 02 Aug 2022 20:05:33: 39000000 INFO @ Tue, 02 Aug 2022 20:05:33: 27000000 INFO @ Tue, 02 Aug 2022 20:05:36: 32000000 INFO @ Tue, 02 Aug 2022 20:05:46: 40000000 INFO @ Tue, 02 Aug 2022 20:05:51: 28000000 INFO @ Tue, 02 Aug 2022 20:05:52: 33000000 INFO @ Tue, 02 Aug 2022 20:05:57: 41000000 INFO @ Tue, 02 Aug 2022 20:06:08: 29000000 INFO @ Tue, 02 Aug 2022 20:06:09: 34000000 INFO @ Tue, 02 Aug 2022 20:06:09: 42000000 INFO @ Tue, 02 Aug 2022 20:06:21: 43000000 INFO @ Tue, 02 Aug 2022 20:06:25: 30000000 INFO @ Tue, 02 Aug 2022 20:06:26: 35000000 INFO @ Tue, 02 Aug 2022 20:06:33: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 20:06:41: 36000000 INFO @ Tue, 02 Aug 2022 20:06:42: 31000000 INFO @ Tue, 02 Aug 2022 20:06:44: 45000000 INFO @ Tue, 02 Aug 2022 20:06:55: 46000000 INFO @ Tue, 02 Aug 2022 20:06:56: 37000000 INFO @ Tue, 02 Aug 2022 20:07:00: 32000000 INFO @ Tue, 02 Aug 2022 20:07:08: 47000000 INFO @ Tue, 02 Aug 2022 20:07:13: 38000000 INFO @ Tue, 02 Aug 2022 20:07:17: 33000000 INFO @ Tue, 02 Aug 2022 20:07:19: 48000000 INFO @ Tue, 02 Aug 2022 20:07:29: 39000000 INFO @ Tue, 02 Aug 2022 20:07:31: 49000000 INFO @ Tue, 02 Aug 2022 20:07:34: 34000000 INFO @ Tue, 02 Aug 2022 20:07:42: 50000000 INFO @ Tue, 02 Aug 2022 20:07:44: 40000000 INFO @ Tue, 02 Aug 2022 20:07:52: 35000000 INFO @ Tue, 02 Aug 2022 20:07:54: 51000000 INFO @ Tue, 02 Aug 2022 20:08:00: 41000000 INFO @ Tue, 02 Aug 2022 20:08:05: 52000000 INFO @ Tue, 02 Aug 2022 20:08:09: 36000000 INFO @ Tue, 02 Aug 2022 20:08:17: 42000000 INFO @ Tue, 02 Aug 2022 20:08:18: 53000000 INFO @ Tue, 02 Aug 2022 20:08:27: 37000000 INFO @ Tue, 02 Aug 2022 20:08:30: 54000000 INFO @ Tue, 02 Aug 2022 20:08:33: 43000000 INFO @ Tue, 02 Aug 2022 20:08:42: 55000000 INFO @ Tue, 02 Aug 2022 20:08:44: 38000000 INFO @ Tue, 02 Aug 2022 20:08:49: 44000000 INFO @ Tue, 02 Aug 2022 20:08:52: 56000000 INFO @ Tue, 02 Aug 2022 20:09:01: 39000000 INFO @ Tue, 02 Aug 2022 20:09:04: #1 tag size is determined as 138 bps INFO @ Tue, 02 Aug 2022 20:09:04: #1 tag size = 138 INFO @ Tue, 02 Aug 2022 20:09:04: #1 total tags in treatment: 25878591 INFO @ Tue, 02 Aug 2022 20:09:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 20:09:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 20:09:04: #1 tags after filtering in treatment: 23348834 INFO @ Tue, 02 Aug 2022 20:09:04: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 02 Aug 2022 20:09:04: #1 finished! INFO @ Tue, 02 Aug 2022 20:09:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 20:09:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:09:05: 45000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 20:09:07: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 20:09:07: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 20:09:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 20:09:18: 40000000 INFO @ Tue, 02 Aug 2022 20:09:21: 46000000 INFO @ Tue, 02 Aug 2022 20:09:36: 41000000 INFO @ Tue, 02 Aug 2022 20:09:37: 47000000 INFO @ Tue, 02 Aug 2022 20:09:53: 42000000 INFO @ Tue, 02 Aug 2022 20:09:53: 48000000 INFO @ Tue, 02 Aug 2022 20:10:09: 49000000 INFO @ Tue, 02 Aug 2022 20:10:09: 43000000 INFO @ Tue, 02 Aug 2022 20:10:22: 44000000 INFO @ Tue, 02 Aug 2022 20:10:26: 50000000 INFO @ Tue, 02 Aug 2022 20:10:34: 45000000 INFO @ Tue, 02 Aug 2022 20:10:41: 51000000 INFO @ Tue, 02 Aug 2022 20:10:44: 46000000 INFO @ Tue, 02 Aug 2022 20:10:55: 47000000 INFO @ Tue, 02 Aug 2022 20:10:57: 52000000 INFO @ Tue, 02 Aug 2022 20:11:06: 48000000 INFO @ Tue, 02 Aug 2022 20:11:12: 53000000 INFO @ Tue, 02 Aug 2022 20:11:17: 49000000 INFO @ Tue, 02 Aug 2022 20:11:28: 50000000 INFO @ Tue, 02 Aug 2022 20:11:28: 54000000 INFO @ Tue, 02 Aug 2022 20:11:41: 51000000 INFO @ Tue, 02 Aug 2022 20:11:44: 55000000 INFO @ Tue, 02 Aug 2022 20:11:53: 52000000 INFO @ Tue, 02 Aug 2022 20:12:01: 56000000 INFO @ Tue, 02 Aug 2022 20:12:05: 53000000 INFO @ Tue, 02 Aug 2022 20:12:17: #1 tag size is determined as 138 bps INFO @ Tue, 02 Aug 2022 20:12:17: #1 tag size = 138 INFO @ Tue, 02 Aug 2022 20:12:17: #1 total tags in treatment: 25878591 INFO @ Tue, 02 Aug 2022 20:12:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 20:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 20:12:17: #1 tags after filtering in treatment: 23348834 INFO @ Tue, 02 Aug 2022 20:12:17: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 02 Aug 2022 20:12:17: #1 finished! INFO @ Tue, 02 Aug 2022 20:12:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 20:12:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:12:17: 54000000 INFO @ Tue, 02 Aug 2022 20:12:19: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 20:12:19: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 20:12:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 20:12:28: 55000000 INFO @ Tue, 02 Aug 2022 20:12:41: 56000000 INFO @ Tue, 02 Aug 2022 20:12:52: #1 tag size is determined as 138 bps INFO @ Tue, 02 Aug 2022 20:12:52: #1 tag size = 138 INFO @ Tue, 02 Aug 2022 20:12:52: #1 total tags in treatment: 25878591 INFO @ Tue, 02 Aug 2022 20:12:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 20:12:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 20:12:53: #1 tags after filtering in treatment: 23348834 INFO @ Tue, 02 Aug 2022 20:12:53: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 02 Aug 2022 20:12:53: #1 finished! INFO @ Tue, 02 Aug 2022 20:12:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 20:12:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:12:55: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 20:12:55: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 20:12:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499352/SRX11499352.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling