Job ID = 6527578 SRX = SRX1149373 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:00:09 prefetch.2.10.7: 1) Downloading 'SRR2162782'... 2020-06-29T13:00:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:01:55 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:01:56 prefetch.2.10.7: 'SRR2162782' is valid 2020-06-29T13:01:56 prefetch.2.10.7: 1) 'SRR2162782' was downloaded successfully 2020-06-29T13:02:48 prefetch.2.10.7: 'SRR2162782' has 10 unresolved dependencies 2020-06-29T13:02:48 prefetch.2.10.7: 2) Downloading 'ncbi-acc:NC_004353.3?vdb-ctx=refseq'... 2020-06-29T13:02:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:01 prefetch.2.10.7: 2) 'ncbi-acc:NC_004353.3?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:03:01 prefetch.2.10.7: 3) Downloading 'ncbi-acc:NC_004354.3?vdb-ctx=refseq'... 2020-06-29T13:03:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:14 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:14 prefetch.2.10.7: 3) 'ncbi-acc:NC_004354.3?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:03:14 prefetch.2.10.7: 4) Downloading 'ncbi-acc:NS_000188.1?vdb-ctx=refseq'... 2020-06-29T13:03:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:26 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:26 prefetch.2.10.7: 4) 'ncbi-acc:NS_000188.1?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:03:26 prefetch.2.10.7: 5) Downloading 'ncbi-acc:NT_033777.2?vdb-ctx=refseq'... 2020-06-29T13:03:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:40 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:40 prefetch.2.10.7: 5) 'ncbi-acc:NT_033777.2?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:03:40 prefetch.2.10.7: 6) Downloading 'ncbi-acc:NT_033778.3?vdb-ctx=refseq'... 2020-06-29T13:03:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:57 prefetch.2.10.7: 6) 'ncbi-acc:NT_033778.3?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:03:57 prefetch.2.10.7: 7) Downloading 'ncbi-acc:NT_033779.4?vdb-ctx=refseq'... 2020-06-29T13:03:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:04:14 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:04:14 prefetch.2.10.7: 7) 'ncbi-acc:NT_033779.4?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:04:14 prefetch.2.10.7: 8) Downloading 'ncbi-acc:NT_037436.3?vdb-ctx=refseq'... 2020-06-29T13:04:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:04:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:04:29 prefetch.2.10.7: 8) 'ncbi-acc:NT_037436.3?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:04:29 prefetch.2.10.7: 9) Downloading 'ncbi-acc:NW_001848856.1?vdb-ctx=refseq'... 2020-06-29T13:04:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:04:39 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:04:39 prefetch.2.10.7: 9) 'ncbi-acc:NW_001848856.1?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:04:39 prefetch.2.10.7: 10) Downloading 'ncbi-acc:NW_001848857.1?vdb-ctx=refseq'... 2020-06-29T13:04:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:04:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:04:52 prefetch.2.10.7: 10) 'ncbi-acc:NW_001848857.1?vdb-ctx=refseq' was downloaded successfully 2020-06-29T13:04:52 prefetch.2.10.7: 11) Downloading 'ncbi-acc:NW_001848858.1?vdb-ctx=refseq'... 2020-06-29T13:04:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:05:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:05:02 prefetch.2.10.7: 11) 'ncbi-acc:NW_001848858.1?vdb-ctx=refseq' was downloaded successfully Read 38878888 spots for SRR2162782/SRR2162782.sra Written 38878888 spots for SRR2162782/SRR2162782.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:41 38878888 reads; of these: 38878888 (100.00%) were unpaired; of these: 611616 (1.57%) aligned 0 times 21642343 (55.67%) aligned exactly 1 time 16624929 (42.76%) aligned >1 times 98.43% overall alignment rate Time searching: 00:12:41 Overall time: 00:12:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6096521 / 38267272 = 0.1593 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:32:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:32:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:32:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:32:06: 1000000 INFO @ Mon, 29 Jun 2020 22:32:11: 2000000 INFO @ Mon, 29 Jun 2020 22:32:15: 3000000 INFO @ Mon, 29 Jun 2020 22:32:20: 4000000 INFO @ Mon, 29 Jun 2020 22:32:24: 5000000 INFO @ Mon, 29 Jun 2020 22:32:29: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:32:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:32:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:32:33: 7000000 INFO @ Mon, 29 Jun 2020 22:32:36: 1000000 INFO @ Mon, 29 Jun 2020 22:32:38: 8000000 INFO @ Mon, 29 Jun 2020 22:32:41: 2000000 INFO @ Mon, 29 Jun 2020 22:32:42: 9000000 INFO @ Mon, 29 Jun 2020 22:32:46: 3000000 INFO @ Mon, 29 Jun 2020 22:32:47: 10000000 INFO @ Mon, 29 Jun 2020 22:32:50: 4000000 INFO @ Mon, 29 Jun 2020 22:32:52: 11000000 INFO @ Mon, 29 Jun 2020 22:32:55: 5000000 INFO @ Mon, 29 Jun 2020 22:32:57: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:33:00: 6000000 INFO @ Mon, 29 Jun 2020 22:33:02: 13000000 INFO @ Mon, 29 Jun 2020 22:33:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:33:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:33:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:33:05: 7000000 INFO @ Mon, 29 Jun 2020 22:33:06: 14000000 INFO @ Mon, 29 Jun 2020 22:33:07: 1000000 INFO @ Mon, 29 Jun 2020 22:33:10: 8000000 INFO @ Mon, 29 Jun 2020 22:33:11: 15000000 INFO @ Mon, 29 Jun 2020 22:33:12: 2000000 INFO @ Mon, 29 Jun 2020 22:33:14: 9000000 INFO @ Mon, 29 Jun 2020 22:33:16: 16000000 INFO @ Mon, 29 Jun 2020 22:33:16: 3000000 INFO @ Mon, 29 Jun 2020 22:33:19: 10000000 INFO @ Mon, 29 Jun 2020 22:33:20: 17000000 INFO @ Mon, 29 Jun 2020 22:33:21: 4000000 INFO @ Mon, 29 Jun 2020 22:33:24: 11000000 INFO @ Mon, 29 Jun 2020 22:33:25: 18000000 INFO @ Mon, 29 Jun 2020 22:33:26: 5000000 INFO @ Mon, 29 Jun 2020 22:33:28: 12000000 INFO @ Mon, 29 Jun 2020 22:33:29: 19000000 INFO @ Mon, 29 Jun 2020 22:33:31: 6000000 INFO @ Mon, 29 Jun 2020 22:33:33: 13000000 INFO @ Mon, 29 Jun 2020 22:33:34: 20000000 INFO @ Mon, 29 Jun 2020 22:33:35: 7000000 INFO @ Mon, 29 Jun 2020 22:33:38: 14000000 INFO @ Mon, 29 Jun 2020 22:33:38: 21000000 INFO @ Mon, 29 Jun 2020 22:33:40: 8000000 INFO @ Mon, 29 Jun 2020 22:33:42: 15000000 INFO @ Mon, 29 Jun 2020 22:33:43: 22000000 INFO @ Mon, 29 Jun 2020 22:33:45: 9000000 INFO @ Mon, 29 Jun 2020 22:33:47: 16000000 INFO @ Mon, 29 Jun 2020 22:33:48: 23000000 INFO @ Mon, 29 Jun 2020 22:33:50: 10000000 INFO @ Mon, 29 Jun 2020 22:33:51: 17000000 INFO @ Mon, 29 Jun 2020 22:33:52: 24000000 INFO @ Mon, 29 Jun 2020 22:33:54: 11000000 INFO @ Mon, 29 Jun 2020 22:33:56: 18000000 INFO @ Mon, 29 Jun 2020 22:33:57: 25000000 INFO @ Mon, 29 Jun 2020 22:33:59: 12000000 INFO @ Mon, 29 Jun 2020 22:34:00: 19000000 INFO @ Mon, 29 Jun 2020 22:34:01: 26000000 INFO @ Mon, 29 Jun 2020 22:34:04: 13000000 INFO @ Mon, 29 Jun 2020 22:34:05: 20000000 INFO @ Mon, 29 Jun 2020 22:34:06: 27000000 INFO @ Mon, 29 Jun 2020 22:34:08: 14000000 INFO @ Mon, 29 Jun 2020 22:34:09: 21000000 INFO @ Mon, 29 Jun 2020 22:34:10: 28000000 INFO @ Mon, 29 Jun 2020 22:34:13: 15000000 INFO @ Mon, 29 Jun 2020 22:34:14: 22000000 INFO @ Mon, 29 Jun 2020 22:34:15: 29000000 INFO @ Mon, 29 Jun 2020 22:34:17: 16000000 INFO @ Mon, 29 Jun 2020 22:34:19: 23000000 INFO @ Mon, 29 Jun 2020 22:34:19: 30000000 INFO @ Mon, 29 Jun 2020 22:34:22: 17000000 INFO @ Mon, 29 Jun 2020 22:34:23: 24000000 INFO @ Mon, 29 Jun 2020 22:34:24: 31000000 INFO @ Mon, 29 Jun 2020 22:34:27: 18000000 INFO @ Mon, 29 Jun 2020 22:34:28: 25000000 INFO @ Mon, 29 Jun 2020 22:34:28: 32000000 INFO @ Mon, 29 Jun 2020 22:34:29: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:34:29: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:34:29: #1 total tags in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:34:29: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:34:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:34:30: #1 tags after filtering in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:34:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:34:30: #1 finished! INFO @ Mon, 29 Jun 2020 22:34:30: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:34:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:34:31: 19000000 INFO @ Mon, 29 Jun 2020 22:34:32: #2 number of paired peaks: 138 WARNING @ Mon, 29 Jun 2020 22:34:32: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Mon, 29 Jun 2020 22:34:32: start model_add_line... INFO @ Mon, 29 Jun 2020 22:34:32: start X-correlation... INFO @ Mon, 29 Jun 2020 22:34:32: end of X-cor INFO @ Mon, 29 Jun 2020 22:34:32: #2 finished! INFO @ Mon, 29 Jun 2020 22:34:32: #2 predicted fragment length is 98 bps INFO @ Mon, 29 Jun 2020 22:34:32: #2 alternative fragment length(s) may be 3,98,130 bps INFO @ Mon, 29 Jun 2020 22:34:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05_model.r WARNING @ Mon, 29 Jun 2020 22:34:32: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 29 Jun 2020 22:34:32: #2 You may need to consider one of the other alternative d(s): 3,98,130 WARNING @ Mon, 29 Jun 2020 22:34:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 29 Jun 2020 22:34:32: #3 Call peaks... INFO @ Mon, 29 Jun 2020 22:34:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 29 Jun 2020 22:34:32: 26000000 INFO @ Mon, 29 Jun 2020 22:34:36: 20000000 INFO @ Mon, 29 Jun 2020 22:34:37: 27000000 INFO @ Mon, 29 Jun 2020 22:34:40: 21000000 INFO @ Mon, 29 Jun 2020 22:34:42: 28000000 INFO @ Mon, 29 Jun 2020 22:34:45: 22000000 INFO @ Mon, 29 Jun 2020 22:34:46: 29000000 INFO @ Mon, 29 Jun 2020 22:34:49: 23000000 INFO @ Mon, 29 Jun 2020 22:34:51: 30000000 INFO @ Mon, 29 Jun 2020 22:34:54: 24000000 INFO @ Mon, 29 Jun 2020 22:34:55: 31000000 INFO @ Mon, 29 Jun 2020 22:34:59: 25000000 INFO @ Mon, 29 Jun 2020 22:34:59: 32000000 INFO @ Mon, 29 Jun 2020 22:35:00: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:35:00: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:35:00: #1 total tags in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:35:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:35:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:35:01: #1 tags after filtering in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:35:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:35:01: #1 finished! INFO @ Mon, 29 Jun 2020 22:35:01: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:35:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:35:03: #2 number of paired peaks: 138 WARNING @ Mon, 29 Jun 2020 22:35:03: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Mon, 29 Jun 2020 22:35:03: start model_add_line... INFO @ Mon, 29 Jun 2020 22:35:03: start X-correlation... INFO @ Mon, 29 Jun 2020 22:35:03: end of X-cor INFO @ Mon, 29 Jun 2020 22:35:03: #2 finished! INFO @ Mon, 29 Jun 2020 22:35:03: #2 predicted fragment length is 98 bps INFO @ Mon, 29 Jun 2020 22:35:03: #2 alternative fragment length(s) may be 3,98,130 bps INFO @ Mon, 29 Jun 2020 22:35:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10_model.r WARNING @ Mon, 29 Jun 2020 22:35:03: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 29 Jun 2020 22:35:03: #2 You may need to consider one of the other alternative d(s): 3,98,130 WARNING @ Mon, 29 Jun 2020 22:35:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 29 Jun 2020 22:35:03: #3 Call peaks... INFO @ Mon, 29 Jun 2020 22:35:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 29 Jun 2020 22:35:03: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:35:08: 27000000 INFO @ Mon, 29 Jun 2020 22:35:12: 28000000 INFO @ Mon, 29 Jun 2020 22:35:17: 29000000 INFO @ Mon, 29 Jun 2020 22:35:22: 30000000 INFO @ Mon, 29 Jun 2020 22:35:26: 31000000 INFO @ Mon, 29 Jun 2020 22:35:29: #3 Call peaks for each chromosome... INFO @ Mon, 29 Jun 2020 22:35:31: 32000000 INFO @ Mon, 29 Jun 2020 22:35:32: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:35:32: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:35:32: #1 total tags in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:35:32: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:35:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:35:32: #1 tags after filtering in treatment: 32170751 INFO @ Mon, 29 Jun 2020 22:35:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:35:32: #1 finished! INFO @ Mon, 29 Jun 2020 22:35:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:35:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:35:34: #2 number of paired peaks: 138 WARNING @ Mon, 29 Jun 2020 22:35:34: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Mon, 29 Jun 2020 22:35:34: start model_add_line... INFO @ Mon, 29 Jun 2020 22:35:34: start X-correlation... INFO @ Mon, 29 Jun 2020 22:35:34: end of X-cor INFO @ Mon, 29 Jun 2020 22:35:34: #2 finished! INFO @ Mon, 29 Jun 2020 22:35:34: #2 predicted fragment length is 98 bps INFO @ Mon, 29 Jun 2020 22:35:34: #2 alternative fragment length(s) may be 3,98,130 bps INFO @ Mon, 29 Jun 2020 22:35:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20_model.r WARNING @ Mon, 29 Jun 2020 22:35:34: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 29 Jun 2020 22:35:34: #2 You may need to consider one of the other alternative d(s): 3,98,130 WARNING @ Mon, 29 Jun 2020 22:35:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 29 Jun 2020 22:35:34: #3 Call peaks... INFO @ Mon, 29 Jun 2020 22:35:34: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 29 Jun 2020 22:35:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05_peaks.xls INFO @ Mon, 29 Jun 2020 22:35:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05_peaks.narrowPeak INFO @ Mon, 29 Jun 2020 22:35:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.05_summits.bed INFO @ Mon, 29 Jun 2020 22:35:57: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (10703 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:36:01: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 22:36:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10_peaks.xls INFO @ Mon, 29 Jun 2020 22:36:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10_peaks.narrowPeak INFO @ Mon, 29 Jun 2020 22:36:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.10_summits.bed INFO @ Mon, 29 Jun 2020 22:36:30: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4722 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:36:31: #3 Call peaks for each chromosome... INFO @ Mon, 29 Jun 2020 22:36:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20_peaks.xls INFO @ Mon, 29 Jun 2020 22:36:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20_peaks.narrowPeak INFO @ Mon, 29 Jun 2020 22:36:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1149373/SRX1149373.20_summits.bed INFO @ Mon, 29 Jun 2020 22:36:58: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1688 records, 4 fields): 2 millis CompletedMACS2peakCalling