Job ID = 1293794 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-02T17:22:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T17:22:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T17:24:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T17:24:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 11,854,057 reads read : 11,854,057 reads written : 11,854,057 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:46 11854057 reads; of these: 11854057 (100.00%) were unpaired; of these: 1179941 (9.95%) aligned 0 times 9541650 (80.49%) aligned exactly 1 time 1132466 (9.55%) aligned >1 times 90.05% overall alignment rate Time searching: 00:02:46 Overall time: 00:02:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7613612 / 10674116 = 0.7133 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:34:02: 1000000 INFO @ Mon, 03 Jun 2019 02:34:03: 1000000 INFO @ Mon, 03 Jun 2019 02:34:03: 1000000 INFO @ Mon, 03 Jun 2019 02:34:11: 2000000 INFO @ Mon, 03 Jun 2019 02:34:13: 2000000 INFO @ Mon, 03 Jun 2019 02:34:13: 2000000 INFO @ Mon, 03 Jun 2019 02:34:19: 3000000 INFO @ Mon, 03 Jun 2019 02:34:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 02:34:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 02:34:19: #1 total tags in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:34:19: #1 tags after filtering in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:34:19: #1 finished! INFO @ Mon, 03 Jun 2019 02:34:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:34:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:34:20: #2 number of paired peaks: 5270 INFO @ Mon, 03 Jun 2019 02:34:20: start model_add_line... INFO @ Mon, 03 Jun 2019 02:34:20: start X-correlation... INFO @ Mon, 03 Jun 2019 02:34:20: end of X-cor INFO @ Mon, 03 Jun 2019 02:34:20: #2 finished! INFO @ Mon, 03 Jun 2019 02:34:20: #2 predicted fragment length is 207 bps INFO @ Mon, 03 Jun 2019 02:34:20: #2 alternative fragment length(s) may be 207 bps INFO @ Mon, 03 Jun 2019 02:34:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10_model.r INFO @ Mon, 03 Jun 2019 02:34:20: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:34:20: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:34:22: 3000000 INFO @ Mon, 03 Jun 2019 02:34:22: 3000000 INFO @ Mon, 03 Jun 2019 02:34:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 02:34:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 02:34:23: #1 total tags in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:34:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:34:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 02:34:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 02:34:23: #1 total tags in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:34:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:34:23: #1 tags after filtering in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:34:23: #1 finished! INFO @ Mon, 03 Jun 2019 02:34:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:34:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:34:23: #1 tags after filtering in treatment: 3060504 INFO @ Mon, 03 Jun 2019 02:34:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:34:23: #1 finished! INFO @ Mon, 03 Jun 2019 02:34:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:34:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:34:24: #2 number of paired peaks: 5270 INFO @ Mon, 03 Jun 2019 02:34:24: start model_add_line... INFO @ Mon, 03 Jun 2019 02:34:24: #2 number of paired peaks: 5270 INFO @ Mon, 03 Jun 2019 02:34:24: start model_add_line... INFO @ Mon, 03 Jun 2019 02:34:24: start X-correlation... INFO @ Mon, 03 Jun 2019 02:34:24: end of X-cor INFO @ Mon, 03 Jun 2019 02:34:24: #2 finished! INFO @ Mon, 03 Jun 2019 02:34:24: #2 predicted fragment length is 207 bps INFO @ Mon, 03 Jun 2019 02:34:24: #2 alternative fragment length(s) may be 207 bps INFO @ Mon, 03 Jun 2019 02:34:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20_model.r INFO @ Mon, 03 Jun 2019 02:34:24: start X-correlation... INFO @ Mon, 03 Jun 2019 02:34:24: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:34:24: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:34:24: end of X-cor INFO @ Mon, 03 Jun 2019 02:34:24: #2 finished! INFO @ Mon, 03 Jun 2019 02:34:24: #2 predicted fragment length is 207 bps INFO @ Mon, 03 Jun 2019 02:34:24: #2 alternative fragment length(s) may be 207 bps INFO @ Mon, 03 Jun 2019 02:34:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05_model.r INFO @ Mon, 03 Jun 2019 02:34:24: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:34:24: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:34:32: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:34:36: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:34:36: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:34:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10_peaks.xls INFO @ Mon, 03 Jun 2019 02:34:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 02:34:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.10_summits.bed INFO @ Mon, 03 Jun 2019 02:34:38: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (3580 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20_peaks.xls INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.20_summits.bed INFO @ Mon, 03 Jun 2019 02:34:42: Done! INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05_peaks.xls INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05_peaks.narrowPeak pass1 - making usageList (12 chroms): 2 millis INFO @ Mon, 03 Jun 2019 02:34:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1134695/SRX1134695.05_summits.bed pass2 - checking and writing primary data (2492 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:34:42: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (4737 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。