Job ID = 9158371 sra ファイルのダウンロード中... Completed: 722200K bytes transferred in 8 seconds (728391K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24517599 spots for /home/okishinya/chipatlas/results/dm3/SRX113331/SRR392952.sra Written 24517599 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:42 24517599 reads; of these: 24517599 (100.00%) were unpaired; of these: 475326 (1.94%) aligned 0 times 19182440 (78.24%) aligned exactly 1 time 4859833 (19.82%) aligned >1 times 98.06% overall alignment rate Time searching: 00:07:42 Overall time: 00:07:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5244877 / 24042273 = 0.2182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:35:49: # Command line: callpeak -t SRX113331.bam -f BAM -g dm -n SRX113331.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113331.20 # format = BAM # ChIP-seq file = ['SRX113331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:35:49: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:35:49: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:35:49: # Command line: callpeak -t SRX113331.bam -f BAM -g dm -n SRX113331.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113331.05 # format = BAM # ChIP-seq file = ['SRX113331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:35:49: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:35:49: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:35:49: # Command line: callpeak -t SRX113331.bam -f BAM -g dm -n SRX113331.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113331.10 # format = BAM # ChIP-seq file = ['SRX113331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:35:49: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:35:49: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:35:55: 1000000 INFO @ Tue, 27 Jun 2017 16:35:55: 1000000 INFO @ Tue, 27 Jun 2017 16:35:56: 1000000 INFO @ Tue, 27 Jun 2017 16:36:01: 2000000 INFO @ Tue, 27 Jun 2017 16:36:02: 2000000 INFO @ Tue, 27 Jun 2017 16:36:02: 2000000 INFO @ Tue, 27 Jun 2017 16:36:07: 3000000 INFO @ Tue, 27 Jun 2017 16:36:08: 3000000 INFO @ Tue, 27 Jun 2017 16:36:09: 3000000 INFO @ Tue, 27 Jun 2017 16:36:14: 4000000 INFO @ Tue, 27 Jun 2017 16:36:14: 4000000 INFO @ Tue, 27 Jun 2017 16:36:16: 4000000 INFO @ Tue, 27 Jun 2017 16:36:20: 5000000 INFO @ Tue, 27 Jun 2017 16:36:21: 5000000 INFO @ Tue, 27 Jun 2017 16:36:23: 5000000 INFO @ Tue, 27 Jun 2017 16:36:26: 6000000 INFO @ Tue, 27 Jun 2017 16:36:27: 6000000 INFO @ Tue, 27 Jun 2017 16:36:29: 6000000 INFO @ Tue, 27 Jun 2017 16:36:32: 7000000 INFO @ Tue, 27 Jun 2017 16:36:33: 7000000 INFO @ Tue, 27 Jun 2017 16:36:36: 7000000 INFO @ Tue, 27 Jun 2017 16:36:38: 8000000 INFO @ Tue, 27 Jun 2017 16:36:40: 8000000 INFO @ Tue, 27 Jun 2017 16:36:42: 8000000 INFO @ Tue, 27 Jun 2017 16:36:44: 9000000 INFO @ Tue, 27 Jun 2017 16:36:46: 9000000 INFO @ Tue, 27 Jun 2017 16:36:49: 9000000 INFO @ Tue, 27 Jun 2017 16:36:50: 10000000 INFO @ Tue, 27 Jun 2017 16:36:52: 10000000 INFO @ Tue, 27 Jun 2017 16:36:55: 10000000 INFO @ Tue, 27 Jun 2017 16:36:56: 11000000 INFO @ Tue, 27 Jun 2017 16:36:58: 11000000 INFO @ Tue, 27 Jun 2017 16:37:02: 12000000 INFO @ Tue, 27 Jun 2017 16:37:02: 11000000 INFO @ Tue, 27 Jun 2017 16:37:04: 12000000 INFO @ Tue, 27 Jun 2017 16:37:08: 13000000 INFO @ Tue, 27 Jun 2017 16:37:08: 12000000 INFO @ Tue, 27 Jun 2017 16:37:10: 13000000 INFO @ Tue, 27 Jun 2017 16:37:14: 14000000 INFO @ Tue, 27 Jun 2017 16:37:15: 13000000 INFO @ Tue, 27 Jun 2017 16:37:17: 14000000 INFO @ Tue, 27 Jun 2017 16:37:20: 15000000 INFO @ Tue, 27 Jun 2017 16:37:22: 14000000 INFO @ Tue, 27 Jun 2017 16:37:23: 15000000 INFO @ Tue, 27 Jun 2017 16:37:26: 16000000 INFO @ Tue, 27 Jun 2017 16:37:28: 15000000 INFO @ Tue, 27 Jun 2017 16:37:29: 16000000 INFO @ Tue, 27 Jun 2017 16:37:32: 17000000 INFO @ Tue, 27 Jun 2017 16:37:35: 16000000 INFO @ Tue, 27 Jun 2017 16:37:35: 17000000 INFO @ Tue, 27 Jun 2017 16:37:38: 18000000 INFO @ Tue, 27 Jun 2017 16:37:41: 17000000 INFO @ Tue, 27 Jun 2017 16:37:41: 18000000 INFO @ Tue, 27 Jun 2017 16:37:43: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:43: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:43: #1 total tags in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:43: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:43: #1 tags after filtering in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:43: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:43: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:44: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 16:37:44: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:44: Process for pairing-model is terminated! cat: SRX113331.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113331.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:37:46: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:46: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:46: #1 total tags in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:46: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:47: #1 tags after filtering in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:47: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:47: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:48: 18000000 INFO @ Tue, 27 Jun 2017 16:37:48: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 16:37:48: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:48: Process for pairing-model is terminated! cat: SRX113331.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113331.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:37:53: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:53: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:53: #1 total tags in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:53: #1 tags after filtering in treatment: 18797396 INFO @ Tue, 27 Jun 2017 16:37:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:53: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:54: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 16:37:54: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:54: Process for pairing-model is terminated! cat: SRX113331.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113331.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113331.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。