Job ID = 9158369 sra ファイルのダウンロード中... Completed: 1041032K bytes transferred in 9 seconds (891107K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 30861048 spots for /home/okishinya/chipatlas/results/dm3/SRX113329/SRR392949.sra Written 30861048 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:42 30861048 reads; of these: 30861048 (100.00%) were unpaired; of these: 3581809 (11.61%) aligned 0 times 22320574 (72.33%) aligned exactly 1 time 4958665 (16.07%) aligned >1 times 88.39% overall alignment rate Time searching: 00:09:42 Overall time: 00:09:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5592313 / 27279239 = 0.2050 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:38:40: # Command line: callpeak -t SRX113329.bam -f BAM -g dm -n SRX113329.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113329.05 # format = BAM # ChIP-seq file = ['SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:38:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:38:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:38:40: # Command line: callpeak -t SRX113329.bam -f BAM -g dm -n SRX113329.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113329.10 # format = BAM # ChIP-seq file = ['SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:38:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:38:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:38:40: # Command line: callpeak -t SRX113329.bam -f BAM -g dm -n SRX113329.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113329.20 # format = BAM # ChIP-seq file = ['SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:38:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:38:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:38:47: 1000000 INFO @ Tue, 27 Jun 2017 16:38:48: 1000000 INFO @ Tue, 27 Jun 2017 16:38:48: 1000000 INFO @ Tue, 27 Jun 2017 16:38:54: 2000000 INFO @ Tue, 27 Jun 2017 16:38:56: 2000000 INFO @ Tue, 27 Jun 2017 16:38:56: 2000000 INFO @ Tue, 27 Jun 2017 16:39:00: 3000000 INFO @ Tue, 27 Jun 2017 16:39:04: 3000000 INFO @ Tue, 27 Jun 2017 16:39:04: 3000000 INFO @ Tue, 27 Jun 2017 16:39:07: 4000000 INFO @ Tue, 27 Jun 2017 16:39:11: 4000000 INFO @ Tue, 27 Jun 2017 16:39:11: 4000000 INFO @ Tue, 27 Jun 2017 16:39:14: 5000000 INFO @ Tue, 27 Jun 2017 16:39:19: 5000000 INFO @ Tue, 27 Jun 2017 16:39:19: 5000000 INFO @ Tue, 27 Jun 2017 16:39:20: 6000000 INFO @ Tue, 27 Jun 2017 16:39:27: 6000000 INFO @ Tue, 27 Jun 2017 16:39:27: 6000000 INFO @ Tue, 27 Jun 2017 16:39:27: 7000000 INFO @ Tue, 27 Jun 2017 16:39:34: 8000000 INFO @ Tue, 27 Jun 2017 16:39:34: 7000000 INFO @ Tue, 27 Jun 2017 16:39:34: 7000000 INFO @ Tue, 27 Jun 2017 16:39:41: 9000000 INFO @ Tue, 27 Jun 2017 16:39:43: 8000000 INFO @ Tue, 27 Jun 2017 16:39:43: 8000000 INFO @ Tue, 27 Jun 2017 16:39:49: 10000000 INFO @ Tue, 27 Jun 2017 16:39:53: 9000000 INFO @ Tue, 27 Jun 2017 16:39:53: 9000000 INFO @ Tue, 27 Jun 2017 16:39:57: 11000000 INFO @ Tue, 27 Jun 2017 16:40:02: 10000000 INFO @ Tue, 27 Jun 2017 16:40:02: 10000000 INFO @ Tue, 27 Jun 2017 16:40:04: 12000000 INFO @ Tue, 27 Jun 2017 16:40:10: 11000000 INFO @ Tue, 27 Jun 2017 16:40:10: 11000000 INFO @ Tue, 27 Jun 2017 16:40:11: 13000000 INFO @ Tue, 27 Jun 2017 16:40:18: 12000000 INFO @ Tue, 27 Jun 2017 16:40:18: 12000000 INFO @ Tue, 27 Jun 2017 16:40:18: 14000000 INFO @ Tue, 27 Jun 2017 16:40:25: 15000000 INFO @ Tue, 27 Jun 2017 16:40:26: 13000000 INFO @ Tue, 27 Jun 2017 16:40:26: 13000000 INFO @ Tue, 27 Jun 2017 16:40:32: 16000000 INFO @ Tue, 27 Jun 2017 16:40:34: 14000000 INFO @ Tue, 27 Jun 2017 16:40:34: 14000000 INFO @ Tue, 27 Jun 2017 16:40:39: 17000000 INFO @ Tue, 27 Jun 2017 16:40:42: 15000000 INFO @ Tue, 27 Jun 2017 16:40:42: 15000000 INFO @ Tue, 27 Jun 2017 16:40:46: 18000000 INFO @ Tue, 27 Jun 2017 16:40:50: 16000000 INFO @ Tue, 27 Jun 2017 16:40:50: 16000000 INFO @ Tue, 27 Jun 2017 16:40:53: 19000000 INFO @ Tue, 27 Jun 2017 16:40:59: 17000000 INFO @ Tue, 27 Jun 2017 16:40:59: 17000000 INFO @ Tue, 27 Jun 2017 16:41:00: 20000000 INFO @ Tue, 27 Jun 2017 16:41:07: 18000000 INFO @ Tue, 27 Jun 2017 16:41:07: 18000000 INFO @ Tue, 27 Jun 2017 16:41:08: 21000000 INFO @ Tue, 27 Jun 2017 16:41:12: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:41:12: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:41:12: #1 total tags in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:41:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:41:13: #1 tags after filtering in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:41:13: #1 finished! INFO @ Tue, 27 Jun 2017 16:41:13: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:41:14: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 16:41:14: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:41:14: Process for pairing-model is terminated! cat: SRX113329.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113329.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:41:15: 19000000 INFO @ Tue, 27 Jun 2017 16:41:15: 19000000 INFO @ Tue, 27 Jun 2017 16:41:23: 20000000 INFO @ Tue, 27 Jun 2017 16:41:23: 20000000 INFO @ Tue, 27 Jun 2017 16:41:31: 21000000 INFO @ Tue, 27 Jun 2017 16:41:31: 21000000 INFO @ Tue, 27 Jun 2017 16:41:37: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:41:37: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:41:37: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:41:37: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:41:37: #1 total tags in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:37: #1 total tags in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:37: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:41:37: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:41:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:41:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:41:37: #1 tags after filtering in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:37: #1 tags after filtering in treatment: 21686926 INFO @ Tue, 27 Jun 2017 16:41:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:41:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:41:37: #1 finished! INFO @ Tue, 27 Jun 2017 16:41:37: #1 finished! INFO @ Tue, 27 Jun 2017 16:41:37: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:41:37: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:41:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:41:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:41:39: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 16:41:39: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:41:39: Process for pairing-model is terminated! cat: SRX113329.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis INFO @ Tue, 27 Jun 2017 16:41:39: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 16:41:39: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:41:39: Process for pairing-model is terminated! needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113329.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX113329.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113329.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113329.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。