Job ID = 1293776 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,528,655 reads read : 22,528,655 reads written : 22,528,655 spots read : 23,857,543 reads read : 23,857,543 reads written : 23,857,543 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:54 46386198 reads; of these: 46386198 (100.00%) were unpaired; of these: 3013301 (6.50%) aligned 0 times 30153991 (65.01%) aligned exactly 1 time 13218906 (28.50%) aligned >1 times 93.50% overall alignment rate Time searching: 00:17:54 Overall time: 00:17:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15635776 / 43372897 = 0.3605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:48:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:48:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:48:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:48:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:48:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:48:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:48:39: 1000000 INFO @ Mon, 03 Jun 2019 02:48:39: 1000000 INFO @ Mon, 03 Jun 2019 02:48:39: 1000000 INFO @ Mon, 03 Jun 2019 02:48:46: 2000000 INFO @ Mon, 03 Jun 2019 02:48:46: 2000000 INFO @ Mon, 03 Jun 2019 02:48:47: 2000000 INFO @ Mon, 03 Jun 2019 02:48:53: 3000000 INFO @ Mon, 03 Jun 2019 02:48:54: 3000000 INFO @ Mon, 03 Jun 2019 02:48:54: 3000000 INFO @ Mon, 03 Jun 2019 02:49:01: 4000000 INFO @ Mon, 03 Jun 2019 02:49:01: 4000000 INFO @ Mon, 03 Jun 2019 02:49:02: 4000000 INFO @ Mon, 03 Jun 2019 02:49:08: 5000000 INFO @ Mon, 03 Jun 2019 02:49:09: 5000000 INFO @ Mon, 03 Jun 2019 02:49:10: 5000000 INFO @ Mon, 03 Jun 2019 02:49:15: 6000000 INFO @ Mon, 03 Jun 2019 02:49:16: 6000000 INFO @ Mon, 03 Jun 2019 02:49:17: 6000000 INFO @ Mon, 03 Jun 2019 02:49:22: 7000000 INFO @ Mon, 03 Jun 2019 02:49:23: 7000000 INFO @ Mon, 03 Jun 2019 02:49:25: 7000000 INFO @ Mon, 03 Jun 2019 02:49:29: 8000000 INFO @ Mon, 03 Jun 2019 02:49:31: 8000000 INFO @ Mon, 03 Jun 2019 02:49:32: 8000000 INFO @ Mon, 03 Jun 2019 02:49:36: 9000000 INFO @ Mon, 03 Jun 2019 02:49:38: 9000000 INFO @ Mon, 03 Jun 2019 02:49:40: 9000000 INFO @ Mon, 03 Jun 2019 02:49:43: 10000000 INFO @ Mon, 03 Jun 2019 02:49:46: 10000000 INFO @ Mon, 03 Jun 2019 02:49:48: 10000000 INFO @ Mon, 03 Jun 2019 02:49:50: 11000000 INFO @ Mon, 03 Jun 2019 02:49:53: 11000000 INFO @ Mon, 03 Jun 2019 02:49:55: 11000000 INFO @ Mon, 03 Jun 2019 02:49:57: 12000000 INFO @ Mon, 03 Jun 2019 02:50:01: 12000000 INFO @ Mon, 03 Jun 2019 02:50:03: 12000000 INFO @ Mon, 03 Jun 2019 02:50:04: 13000000 INFO @ Mon, 03 Jun 2019 02:50:08: 13000000 INFO @ Mon, 03 Jun 2019 02:50:11: 13000000 INFO @ Mon, 03 Jun 2019 02:50:11: 14000000 INFO @ Mon, 03 Jun 2019 02:50:16: 14000000 INFO @ Mon, 03 Jun 2019 02:50:18: 15000000 INFO @ Mon, 03 Jun 2019 02:50:18: 14000000 INFO @ Mon, 03 Jun 2019 02:50:23: 15000000 INFO @ Mon, 03 Jun 2019 02:50:25: 16000000 INFO @ Mon, 03 Jun 2019 02:50:26: 15000000 INFO @ Mon, 03 Jun 2019 02:50:30: 16000000 INFO @ Mon, 03 Jun 2019 02:50:32: 17000000 INFO @ Mon, 03 Jun 2019 02:50:34: 16000000 INFO @ Mon, 03 Jun 2019 02:50:38: 17000000 INFO @ Mon, 03 Jun 2019 02:50:39: 18000000 INFO @ Mon, 03 Jun 2019 02:50:41: 17000000 INFO @ Mon, 03 Jun 2019 02:50:45: 18000000 INFO @ Mon, 03 Jun 2019 02:50:46: 19000000 INFO @ Mon, 03 Jun 2019 02:50:49: 18000000 INFO @ Mon, 03 Jun 2019 02:50:53: 19000000 INFO @ Mon, 03 Jun 2019 02:50:53: 20000000 INFO @ Mon, 03 Jun 2019 02:50:56: 19000000 INFO @ Mon, 03 Jun 2019 02:51:00: 20000000 INFO @ Mon, 03 Jun 2019 02:51:00: 21000000 INFO @ Mon, 03 Jun 2019 02:51:04: 20000000 INFO @ Mon, 03 Jun 2019 02:51:07: 22000000 INFO @ Mon, 03 Jun 2019 02:51:08: 21000000 INFO @ Mon, 03 Jun 2019 02:51:12: 21000000 INFO @ Mon, 03 Jun 2019 02:51:14: 23000000 INFO @ Mon, 03 Jun 2019 02:51:15: 22000000 INFO @ Mon, 03 Jun 2019 02:51:19: 22000000 INFO @ Mon, 03 Jun 2019 02:51:21: 24000000 INFO @ Mon, 03 Jun 2019 02:51:23: 23000000 INFO @ Mon, 03 Jun 2019 02:51:27: 23000000 INFO @ Mon, 03 Jun 2019 02:51:29: 25000000 INFO @ Mon, 03 Jun 2019 02:51:30: 24000000 INFO @ Mon, 03 Jun 2019 02:51:35: 24000000 INFO @ Mon, 03 Jun 2019 02:51:36: 26000000 INFO @ Mon, 03 Jun 2019 02:51:38: 25000000 INFO @ Mon, 03 Jun 2019 02:51:42: 25000000 INFO @ Mon, 03 Jun 2019 02:51:43: 27000000 INFO @ Mon, 03 Jun 2019 02:51:45: 26000000 INFO @ Mon, 03 Jun 2019 02:51:48: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:51:48: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:51:48: #1 total tags in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:51:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:51:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:51:49: #1 tags after filtering in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:51:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:51:49: #1 finished! INFO @ Mon, 03 Jun 2019 02:51:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:51:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:51:50: 26000000 INFO @ Mon, 03 Jun 2019 02:51:51: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 02:51:51: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:51:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:51:53: 27000000 INFO @ Mon, 03 Jun 2019 02:51:57: 27000000 INFO @ Mon, 03 Jun 2019 02:51:58: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:51:58: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:51:58: #1 total tags in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:51:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:51:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:51:59: #1 tags after filtering in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:51:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:51:59: #1 finished! INFO @ Mon, 03 Jun 2019 02:51:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:52:01: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 02:52:01: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:52:03: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:52:03: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:52:03: #1 total tags in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:52:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:52:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:52:04: #1 tags after filtering in treatment: 27737121 INFO @ Mon, 03 Jun 2019 02:52:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:52:04: #1 finished! INFO @ Mon, 03 Jun 2019 02:52:04: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:52:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:52:06: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 02:52:06: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:52:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113325/SRX113325.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。