Job ID = 9158354 sra ファイルのダウンロード中... Completed: 1241490K bytes transferred in 12 seconds (811797K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20069041 spots for /home/okishinya/chipatlas/results/dm3/SRX113323/SRR392938.sra Written 20069041 spots total Written 21266162 spots for /home/okishinya/chipatlas/results/dm3/SRX113323/SRR392939.sra Written 21266162 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:15:50 41335203 reads; of these: 41335203 (100.00%) were unpaired; of these: 2736729 (6.62%) aligned 0 times 28086357 (67.95%) aligned exactly 1 time 10512117 (25.43%) aligned >1 times 93.38% overall alignment rate Time searching: 00:15:51 Overall time: 00:15:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13495531 / 38598474 = 0.3496 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:41:52: # Command line: callpeak -t SRX113323.bam -f BAM -g dm -n SRX113323.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113323.20 # format = BAM # ChIP-seq file = ['SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:41:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:41:52: # Command line: callpeak -t SRX113323.bam -f BAM -g dm -n SRX113323.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113323.10 # format = BAM # ChIP-seq file = ['SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:41:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:41:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:41:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:41:52: # Command line: callpeak -t SRX113323.bam -f BAM -g dm -n SRX113323.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113323.05 # format = BAM # ChIP-seq file = ['SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:41:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:41:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:41:58: 1000000 INFO @ Tue, 27 Jun 2017 16:41:58: 1000000 INFO @ Tue, 27 Jun 2017 16:41:58: 1000000 INFO @ Tue, 27 Jun 2017 16:42:05: 2000000 INFO @ Tue, 27 Jun 2017 16:42:05: 2000000 INFO @ Tue, 27 Jun 2017 16:42:05: 2000000 INFO @ Tue, 27 Jun 2017 16:42:11: 3000000 INFO @ Tue, 27 Jun 2017 16:42:11: 3000000 INFO @ Tue, 27 Jun 2017 16:42:12: 3000000 INFO @ Tue, 27 Jun 2017 16:42:18: 4000000 INFO @ Tue, 27 Jun 2017 16:42:18: 4000000 INFO @ Tue, 27 Jun 2017 16:42:19: 4000000 INFO @ Tue, 27 Jun 2017 16:42:24: 5000000 INFO @ Tue, 27 Jun 2017 16:42:25: 5000000 INFO @ Tue, 27 Jun 2017 16:42:26: 5000000 INFO @ Tue, 27 Jun 2017 16:42:30: 6000000 INFO @ Tue, 27 Jun 2017 16:42:31: 6000000 INFO @ Tue, 27 Jun 2017 16:42:33: 6000000 INFO @ Tue, 27 Jun 2017 16:42:37: 7000000 INFO @ Tue, 27 Jun 2017 16:42:38: 7000000 INFO @ Tue, 27 Jun 2017 16:42:40: 7000000 INFO @ Tue, 27 Jun 2017 16:42:43: 8000000 INFO @ Tue, 27 Jun 2017 16:42:45: 8000000 INFO @ Tue, 27 Jun 2017 16:42:47: 8000000 INFO @ Tue, 27 Jun 2017 16:42:49: 9000000 INFO @ Tue, 27 Jun 2017 16:42:52: 9000000 INFO @ Tue, 27 Jun 2017 16:42:54: 9000000 INFO @ Tue, 27 Jun 2017 16:42:56: 10000000 INFO @ Tue, 27 Jun 2017 16:42:58: 10000000 INFO @ Tue, 27 Jun 2017 16:43:01: 10000000 INFO @ Tue, 27 Jun 2017 16:43:02: 11000000 INFO @ Tue, 27 Jun 2017 16:43:05: 11000000 INFO @ Tue, 27 Jun 2017 16:43:08: 11000000 INFO @ Tue, 27 Jun 2017 16:43:09: 12000000 INFO @ Tue, 27 Jun 2017 16:43:12: 12000000 INFO @ Tue, 27 Jun 2017 16:43:14: 12000000 INFO @ Tue, 27 Jun 2017 16:43:16: 13000000 INFO @ Tue, 27 Jun 2017 16:43:19: 13000000 INFO @ Tue, 27 Jun 2017 16:43:21: 13000000 INFO @ Tue, 27 Jun 2017 16:43:22: 14000000 INFO @ Tue, 27 Jun 2017 16:43:25: 14000000 INFO @ Tue, 27 Jun 2017 16:43:28: 15000000 INFO @ Tue, 27 Jun 2017 16:43:28: 14000000 INFO @ Tue, 27 Jun 2017 16:43:32: 15000000 INFO @ Tue, 27 Jun 2017 16:43:35: 16000000 INFO @ Tue, 27 Jun 2017 16:43:35: 15000000 INFO @ Tue, 27 Jun 2017 16:43:39: 16000000 INFO @ Tue, 27 Jun 2017 16:43:41: 17000000 INFO @ Tue, 27 Jun 2017 16:43:42: 16000000 INFO @ Tue, 27 Jun 2017 16:43:46: 17000000 INFO @ Tue, 27 Jun 2017 16:43:47: 18000000 INFO @ Tue, 27 Jun 2017 16:43:49: 17000000 INFO @ Tue, 27 Jun 2017 16:43:52: 18000000 INFO @ Tue, 27 Jun 2017 16:43:53: 19000000 INFO @ Tue, 27 Jun 2017 16:43:56: 18000000 INFO @ Tue, 27 Jun 2017 16:43:59: 19000000 INFO @ Tue, 27 Jun 2017 16:44:00: 20000000 INFO @ Tue, 27 Jun 2017 16:44:03: 19000000 INFO @ Tue, 27 Jun 2017 16:44:06: 20000000 INFO @ Tue, 27 Jun 2017 16:44:06: 21000000 INFO @ Tue, 27 Jun 2017 16:44:10: 20000000 INFO @ Tue, 27 Jun 2017 16:44:12: 22000000 INFO @ Tue, 27 Jun 2017 16:44:13: 21000000 INFO @ Tue, 27 Jun 2017 16:44:17: 21000000 INFO @ Tue, 27 Jun 2017 16:44:19: 23000000 INFO @ Tue, 27 Jun 2017 16:44:19: 22000000 INFO @ Tue, 27 Jun 2017 16:44:24: 22000000 INFO @ Tue, 27 Jun 2017 16:44:25: 24000000 INFO @ Tue, 27 Jun 2017 16:44:26: 23000000 INFO @ Tue, 27 Jun 2017 16:44:31: 23000000 INFO @ Tue, 27 Jun 2017 16:44:32: 25000000 INFO @ Tue, 27 Jun 2017 16:44:32: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:44:32: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:44:32: #1 total tags in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:32: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:44:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:44:33: 24000000 INFO @ Tue, 27 Jun 2017 16:44:33: #1 tags after filtering in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:44:33: #1 finished! INFO @ Tue, 27 Jun 2017 16:44:33: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:44:34: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:44:34: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:44:34: Process for pairing-model is terminated! cat: SRX113323.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113323.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:44:38: 24000000 INFO @ Tue, 27 Jun 2017 16:44:40: 25000000 INFO @ Tue, 27 Jun 2017 16:44:40: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:44:40: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:44:40: #1 total tags in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:40: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:44:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:44:41: #1 tags after filtering in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:44:41: #1 finished! INFO @ Tue, 27 Jun 2017 16:44:41: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:44:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:44:43: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:44:43: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:44:43: Process for pairing-model is terminated! cat: SRX113323.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113323.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:44:44: 25000000 INFO @ Tue, 27 Jun 2017 16:44:45: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:44:45: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:44:45: #1 total tags in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:45: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:44:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:44:46: #1 tags after filtering in treatment: 25102943 INFO @ Tue, 27 Jun 2017 16:44:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:44:46: #1 finished! INFO @ Tue, 27 Jun 2017 16:44:46: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:44:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:44:47: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:44:47: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:44:47: Process for pairing-model is terminated! cat: SRX113323.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113323.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113323.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。