Job ID = 9158351 sra ファイルのダウンロード中... Completed: 606138K bytes transferred in 7 seconds (620768K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20636266 spots for /home/okishinya/chipatlas/results/dm3/SRX113318/SRR392930.sra Written 20636266 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:23 20636266 reads; of these: 20636266 (100.00%) were unpaired; of these: 206299 (1.00%) aligned 0 times 13986296 (67.78%) aligned exactly 1 time 6443671 (31.22%) aligned >1 times 99.00% overall alignment rate Time searching: 00:08:23 Overall time: 00:08:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3742907 / 20429967 = 0.1832 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:29:05: # Command line: callpeak -t SRX113318.bam -f BAM -g dm -n SRX113318.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113318.20 # format = BAM # ChIP-seq file = ['SRX113318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:29:05: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:29:05: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:29:05: # Command line: callpeak -t SRX113318.bam -f BAM -g dm -n SRX113318.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113318.05 # format = BAM # ChIP-seq file = ['SRX113318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:29:05: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:29:05: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:29:05: # Command line: callpeak -t SRX113318.bam -f BAM -g dm -n SRX113318.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113318.10 # format = BAM # ChIP-seq file = ['SRX113318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:29:05: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:29:05: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:29:12: 1000000 INFO @ Tue, 27 Jun 2017 16:29:12: 1000000 INFO @ Tue, 27 Jun 2017 16:29:12: 1000000 INFO @ Tue, 27 Jun 2017 16:29:19: 2000000 INFO @ Tue, 27 Jun 2017 16:29:20: 2000000 INFO @ Tue, 27 Jun 2017 16:29:20: 2000000 INFO @ Tue, 27 Jun 2017 16:29:27: 3000000 INFO @ Tue, 27 Jun 2017 16:29:28: 3000000 INFO @ Tue, 27 Jun 2017 16:29:28: 3000000 INFO @ Tue, 27 Jun 2017 16:29:35: 4000000 INFO @ Tue, 27 Jun 2017 16:29:36: 4000000 INFO @ Tue, 27 Jun 2017 16:29:37: 4000000 INFO @ Tue, 27 Jun 2017 16:29:43: 5000000 INFO @ Tue, 27 Jun 2017 16:29:44: 5000000 INFO @ Tue, 27 Jun 2017 16:29:45: 5000000 INFO @ Tue, 27 Jun 2017 16:29:51: 6000000 INFO @ Tue, 27 Jun 2017 16:29:52: 6000000 INFO @ Tue, 27 Jun 2017 16:29:53: 6000000 INFO @ Tue, 27 Jun 2017 16:29:59: 7000000 INFO @ Tue, 27 Jun 2017 16:30:00: 7000000 INFO @ Tue, 27 Jun 2017 16:30:01: 7000000 INFO @ Tue, 27 Jun 2017 16:30:06: 8000000 INFO @ Tue, 27 Jun 2017 16:30:08: 8000000 INFO @ Tue, 27 Jun 2017 16:30:10: 8000000 INFO @ Tue, 27 Jun 2017 16:30:14: 9000000 INFO @ Tue, 27 Jun 2017 16:30:17: 9000000 INFO @ Tue, 27 Jun 2017 16:30:18: 9000000 INFO @ Tue, 27 Jun 2017 16:30:22: 10000000 INFO @ Tue, 27 Jun 2017 16:30:25: 10000000 INFO @ Tue, 27 Jun 2017 16:30:26: 10000000 INFO @ Tue, 27 Jun 2017 16:30:30: 11000000 INFO @ Tue, 27 Jun 2017 16:30:33: 11000000 INFO @ Tue, 27 Jun 2017 16:30:34: 11000000 INFO @ Tue, 27 Jun 2017 16:30:38: 12000000 INFO @ Tue, 27 Jun 2017 16:30:41: 12000000 INFO @ Tue, 27 Jun 2017 16:30:42: 12000000 INFO @ Tue, 27 Jun 2017 16:30:46: 13000000 INFO @ Tue, 27 Jun 2017 16:30:49: 13000000 INFO @ Tue, 27 Jun 2017 16:30:51: 13000000 INFO @ Tue, 27 Jun 2017 16:30:53: 14000000 INFO @ Tue, 27 Jun 2017 16:30:57: 14000000 INFO @ Tue, 27 Jun 2017 16:30:59: 14000000 INFO @ Tue, 27 Jun 2017 16:31:01: 15000000 INFO @ Tue, 27 Jun 2017 16:31:05: 15000000 INFO @ Tue, 27 Jun 2017 16:31:07: 15000000 INFO @ Tue, 27 Jun 2017 16:31:09: 16000000 INFO @ Tue, 27 Jun 2017 16:31:14: 16000000 INFO @ Tue, 27 Jun 2017 16:31:15: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:31:15: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:31:15: #1 total tags in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:15: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:31:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:31:15: #1 tags after filtering in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:31:15: #1 finished! INFO @ Tue, 27 Jun 2017 16:31:15: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:31:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:31:15: 16000000 INFO @ Tue, 27 Jun 2017 16:31:16: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 16:31:16: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:31:16: Process for pairing-model is terminated! cat: SRX113318.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113318.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:31:19: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:31:19: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:31:19: #1 total tags in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:19: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:31:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:31:19: #1 tags after filtering in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:31:19: #1 finished! INFO @ Tue, 27 Jun 2017 16:31:19: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:31:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:31:21: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 16:31:21: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:31:21: Process for pairing-model is terminated! cat: SRX113318.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113318.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:31:21: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:31:21: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:31:21: #1 total tags in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:21: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:31:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:31:21: #1 tags after filtering in treatment: 16687060 INFO @ Tue, 27 Jun 2017 16:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:31:21: #1 finished! INFO @ Tue, 27 Jun 2017 16:31:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:31:22: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 16:31:22: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:31:22: Process for pairing-model is terminated! cat: SRX113318.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113318.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113318.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。