Job ID = 9158345 sra ファイルのダウンロード中... Completed: 649696K bytes transferred in 9 seconds (566440K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21723799 spots for /home/okishinya/chipatlas/results/dm3/SRX113314/SRR392924.sra Written 21723799 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:37 21723799 reads; of these: 21723799 (100.00%) were unpaired; of these: 98884 (0.46%) aligned 0 times 19003129 (87.48%) aligned exactly 1 time 2621786 (12.07%) aligned >1 times 99.54% overall alignment rate Time searching: 00:05:37 Overall time: 00:05:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4159006 / 21624915 = 0.1923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:25:55: # Command line: callpeak -t SRX113314.bam -f BAM -g dm -n SRX113314.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113314.05 # format = BAM # ChIP-seq file = ['SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:25:55: # Command line: callpeak -t SRX113314.bam -f BAM -g dm -n SRX113314.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113314.20 # format = BAM # ChIP-seq file = ['SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:25:55: # Command line: callpeak -t SRX113314.bam -f BAM -g dm -n SRX113314.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113314.10 # format = BAM # ChIP-seq file = ['SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:25:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:25:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:25:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:25:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:25:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:25:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:26:03: 1000000 INFO @ Tue, 27 Jun 2017 16:26:03: 1000000 INFO @ Tue, 27 Jun 2017 16:26:03: 1000000 INFO @ Tue, 27 Jun 2017 16:26:10: 2000000 INFO @ Tue, 27 Jun 2017 16:26:10: 2000000 INFO @ Tue, 27 Jun 2017 16:26:10: 2000000 INFO @ Tue, 27 Jun 2017 16:26:18: 3000000 INFO @ Tue, 27 Jun 2017 16:26:18: 3000000 INFO @ Tue, 27 Jun 2017 16:26:18: 3000000 INFO @ Tue, 27 Jun 2017 16:26:25: 4000000 INFO @ Tue, 27 Jun 2017 16:26:25: 4000000 INFO @ Tue, 27 Jun 2017 16:26:25: 4000000 INFO @ Tue, 27 Jun 2017 16:26:32: 5000000 INFO @ Tue, 27 Jun 2017 16:26:33: 5000000 INFO @ Tue, 27 Jun 2017 16:26:33: 5000000 INFO @ Tue, 27 Jun 2017 16:26:40: 6000000 INFO @ Tue, 27 Jun 2017 16:26:40: 6000000 INFO @ Tue, 27 Jun 2017 16:26:40: 6000000 INFO @ Tue, 27 Jun 2017 16:26:47: 7000000 INFO @ Tue, 27 Jun 2017 16:26:47: 7000000 INFO @ Tue, 27 Jun 2017 16:26:48: 7000000 INFO @ Tue, 27 Jun 2017 16:26:54: 8000000 INFO @ Tue, 27 Jun 2017 16:26:55: 8000000 INFO @ Tue, 27 Jun 2017 16:26:55: 8000000 INFO @ Tue, 27 Jun 2017 16:27:02: 9000000 INFO @ Tue, 27 Jun 2017 16:27:02: 9000000 INFO @ Tue, 27 Jun 2017 16:27:03: 9000000 INFO @ Tue, 27 Jun 2017 16:27:09: 10000000 INFO @ Tue, 27 Jun 2017 16:27:09: 10000000 INFO @ Tue, 27 Jun 2017 16:27:11: 10000000 INFO @ Tue, 27 Jun 2017 16:27:17: 11000000 INFO @ Tue, 27 Jun 2017 16:27:17: 11000000 INFO @ Tue, 27 Jun 2017 16:27:18: 11000000 INFO @ Tue, 27 Jun 2017 16:27:24: 12000000 INFO @ Tue, 27 Jun 2017 16:27:24: 12000000 INFO @ Tue, 27 Jun 2017 16:27:26: 12000000 INFO @ Tue, 27 Jun 2017 16:27:31: 13000000 INFO @ Tue, 27 Jun 2017 16:27:32: 13000000 INFO @ Tue, 27 Jun 2017 16:27:33: 13000000 INFO @ Tue, 27 Jun 2017 16:27:39: 14000000 INFO @ Tue, 27 Jun 2017 16:27:39: 14000000 INFO @ Tue, 27 Jun 2017 16:27:41: 14000000 INFO @ Tue, 27 Jun 2017 16:27:46: 15000000 INFO @ Tue, 27 Jun 2017 16:27:47: 15000000 INFO @ Tue, 27 Jun 2017 16:27:48: 15000000 INFO @ Tue, 27 Jun 2017 16:27:54: 16000000 INFO @ Tue, 27 Jun 2017 16:27:54: 16000000 INFO @ Tue, 27 Jun 2017 16:27:56: 16000000 INFO @ Tue, 27 Jun 2017 16:28:01: 17000000 INFO @ Tue, 27 Jun 2017 16:28:01: 17000000 INFO @ Tue, 27 Jun 2017 16:28:03: 17000000 INFO @ Tue, 27 Jun 2017 16:28:05: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:28:05: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:28:05: #1 total tags in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:05: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:28:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:28:05: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:28:05: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:28:05: #1 total tags in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:05: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:28:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:28:05: #1 tags after filtering in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:28:05: #1 finished! INFO @ Tue, 27 Jun 2017 16:28:05: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:28:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:28:05: #1 tags after filtering in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:28:05: #1 finished! INFO @ Tue, 27 Jun 2017 16:28:05: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:28:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:28:06: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:28:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:28:06: Process for pairing-model is terminated! cat: SRX113314.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113314.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:28:06: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:28:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:28:06: Process for pairing-model is terminated! cat: SRX113314.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113314.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:28:06: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:28:06: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:28:06: #1 total tags in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:28:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:28:07: #1 tags after filtering in treatment: 17465909 INFO @ Tue, 27 Jun 2017 16:28:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:28:07: #1 finished! INFO @ Tue, 27 Jun 2017 16:28:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:28:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:28:08: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:28:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:28:08: Process for pairing-model is terminated! cat: SRX113314.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113314.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113314.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。