Job ID = 1293767 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,802,283 reads read : 21,802,283 reads written : 21,802,283 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 21802283 reads; of these: 21802283 (100.00%) were unpaired; of these: 457317 (2.10%) aligned 0 times 16328448 (74.89%) aligned exactly 1 time 5016518 (23.01%) aligned >1 times 97.90% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4464681 / 21344966 = 0.2092 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:35:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:21: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:21: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:21: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:21: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:21: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:21: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:32: 1000000 INFO @ Mon, 03 Jun 2019 02:35:32: 1000000 INFO @ Mon, 03 Jun 2019 02:35:33: 1000000 INFO @ Mon, 03 Jun 2019 02:35:42: 2000000 INFO @ Mon, 03 Jun 2019 02:35:43: 2000000 INFO @ Mon, 03 Jun 2019 02:35:46: 2000000 INFO @ Mon, 03 Jun 2019 02:35:53: 3000000 INFO @ Mon, 03 Jun 2019 02:35:53: 3000000 INFO @ Mon, 03 Jun 2019 02:35:59: 3000000 INFO @ Mon, 03 Jun 2019 02:36:02: 4000000 INFO @ Mon, 03 Jun 2019 02:36:04: 4000000 INFO @ Mon, 03 Jun 2019 02:36:11: 4000000 INFO @ Mon, 03 Jun 2019 02:36:13: 5000000 INFO @ Mon, 03 Jun 2019 02:36:14: 5000000 INFO @ Mon, 03 Jun 2019 02:36:23: 5000000 INFO @ Mon, 03 Jun 2019 02:36:23: 6000000 INFO @ Mon, 03 Jun 2019 02:36:25: 6000000 INFO @ Mon, 03 Jun 2019 02:36:34: 7000000 INFO @ Mon, 03 Jun 2019 02:36:35: 6000000 INFO @ Mon, 03 Jun 2019 02:36:35: 7000000 INFO @ Mon, 03 Jun 2019 02:36:45: 8000000 INFO @ Mon, 03 Jun 2019 02:36:46: 8000000 INFO @ Mon, 03 Jun 2019 02:36:47: 7000000 INFO @ Mon, 03 Jun 2019 02:36:55: 9000000 INFO @ Mon, 03 Jun 2019 02:36:57: 9000000 INFO @ Mon, 03 Jun 2019 02:37:00: 8000000 INFO @ Mon, 03 Jun 2019 02:37:04: 10000000 INFO @ Mon, 03 Jun 2019 02:37:08: 10000000 INFO @ Mon, 03 Jun 2019 02:37:12: 9000000 INFO @ Mon, 03 Jun 2019 02:37:14: 11000000 INFO @ Mon, 03 Jun 2019 02:37:19: 11000000 INFO @ Mon, 03 Jun 2019 02:37:23: 12000000 INFO @ Mon, 03 Jun 2019 02:37:25: 10000000 INFO @ Mon, 03 Jun 2019 02:37:30: 12000000 INFO @ Mon, 03 Jun 2019 02:37:31: 13000000 INFO @ Mon, 03 Jun 2019 02:37:38: 11000000 INFO @ Mon, 03 Jun 2019 02:37:40: 14000000 INFO @ Mon, 03 Jun 2019 02:37:40: 13000000 INFO @ Mon, 03 Jun 2019 02:37:49: 15000000 INFO @ Mon, 03 Jun 2019 02:37:50: 12000000 INFO @ Mon, 03 Jun 2019 02:37:51: 14000000 INFO @ Mon, 03 Jun 2019 02:37:57: 16000000 INFO @ Mon, 03 Jun 2019 02:38:02: 15000000 INFO @ Mon, 03 Jun 2019 02:38:03: 13000000 INFO @ Mon, 03 Jun 2019 02:38:05: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:38:05: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:38:05: #1 total tags in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:38:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:38:05: #1 tags after filtering in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:38:05: #1 finished! INFO @ Mon, 03 Jun 2019 02:38:05: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:38:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:38:07: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 02:38:07: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:38:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:38:12: 16000000 INFO @ Mon, 03 Jun 2019 02:38:16: 14000000 INFO @ Mon, 03 Jun 2019 02:38:21: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:38:21: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:38:21: #1 total tags in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:38:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:38:22: #1 tags after filtering in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:38:22: #1 finished! INFO @ Mon, 03 Jun 2019 02:38:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:38:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:38:23: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 02:38:23: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:38:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:38:28: 15000000 INFO @ Mon, 03 Jun 2019 02:38:41: 16000000 INFO @ Mon, 03 Jun 2019 02:38:51: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:38:51: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:38:51: #1 total tags in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:38:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:38:51: #1 tags after filtering in treatment: 16880285 INFO @ Mon, 03 Jun 2019 02:38:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:38:51: #1 finished! INFO @ Mon, 03 Jun 2019 02:38:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:38:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:38:53: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 02:38:53: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:38:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113308/SRX113308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。