Job ID = 9158334 sra ファイルのダウンロード中... Completed: 644775K bytes transferred in 8 seconds (600400K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19875673 spots for /home/okishinya/chipatlas/results/dm3/SRX113304/SRR392909.sra Written 19875673 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 19875673 reads; of these: 19875673 (100.00%) were unpaired; of these: 806204 (4.06%) aligned 0 times 15987168 (80.44%) aligned exactly 1 time 3082301 (15.51%) aligned >1 times 95.94% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3389526 / 19069469 = 0.1777 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:23:32: # Command line: callpeak -t SRX113304.bam -f BAM -g dm -n SRX113304.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113304.10 # format = BAM # ChIP-seq file = ['SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:32: # Command line: callpeak -t SRX113304.bam -f BAM -g dm -n SRX113304.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113304.05 # format = BAM # ChIP-seq file = ['SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:32: # Command line: callpeak -t SRX113304.bam -f BAM -g dm -n SRX113304.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113304.20 # format = BAM # ChIP-seq file = ['SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:38: 1000000 INFO @ Tue, 27 Jun 2017 16:23:38: 1000000 INFO @ Tue, 27 Jun 2017 16:23:39: 1000000 INFO @ Tue, 27 Jun 2017 16:23:45: 2000000 INFO @ Tue, 27 Jun 2017 16:23:45: 2000000 INFO @ Tue, 27 Jun 2017 16:23:47: 2000000 INFO @ Tue, 27 Jun 2017 16:23:52: 3000000 INFO @ Tue, 27 Jun 2017 16:23:52: 3000000 INFO @ Tue, 27 Jun 2017 16:23:54: 3000000 INFO @ Tue, 27 Jun 2017 16:23:59: 4000000 INFO @ Tue, 27 Jun 2017 16:23:59: 4000000 INFO @ Tue, 27 Jun 2017 16:24:02: 4000000 INFO @ Tue, 27 Jun 2017 16:24:06: 5000000 INFO @ Tue, 27 Jun 2017 16:24:06: 5000000 INFO @ Tue, 27 Jun 2017 16:24:10: 5000000 INFO @ Tue, 27 Jun 2017 16:24:13: 6000000 INFO @ Tue, 27 Jun 2017 16:24:13: 6000000 INFO @ Tue, 27 Jun 2017 16:24:17: 6000000 INFO @ Tue, 27 Jun 2017 16:24:20: 7000000 INFO @ Tue, 27 Jun 2017 16:24:21: 7000000 INFO @ Tue, 27 Jun 2017 16:24:23: 7000000 INFO @ Tue, 27 Jun 2017 16:24:27: 8000000 INFO @ Tue, 27 Jun 2017 16:24:28: 8000000 INFO @ Tue, 27 Jun 2017 16:24:30: 8000000 INFO @ Tue, 27 Jun 2017 16:24:33: 9000000 INFO @ Tue, 27 Jun 2017 16:24:35: 9000000 INFO @ Tue, 27 Jun 2017 16:24:36: 9000000 INFO @ Tue, 27 Jun 2017 16:24:40: 10000000 INFO @ Tue, 27 Jun 2017 16:24:42: 10000000 INFO @ Tue, 27 Jun 2017 16:24:43: 10000000 INFO @ Tue, 27 Jun 2017 16:24:46: 11000000 INFO @ Tue, 27 Jun 2017 16:24:49: 11000000 INFO @ Tue, 27 Jun 2017 16:24:50: 11000000 INFO @ Tue, 27 Jun 2017 16:24:53: 12000000 INFO @ Tue, 27 Jun 2017 16:24:56: 12000000 INFO @ Tue, 27 Jun 2017 16:24:56: 12000000 INFO @ Tue, 27 Jun 2017 16:24:59: 13000000 INFO @ Tue, 27 Jun 2017 16:25:02: 13000000 INFO @ Tue, 27 Jun 2017 16:25:03: 13000000 INFO @ Tue, 27 Jun 2017 16:25:06: 14000000 INFO @ Tue, 27 Jun 2017 16:25:09: 14000000 INFO @ Tue, 27 Jun 2017 16:25:09: 14000000 INFO @ Tue, 27 Jun 2017 16:25:12: 15000000 INFO @ Tue, 27 Jun 2017 16:25:15: 15000000 INFO @ Tue, 27 Jun 2017 16:25:16: 15000000 INFO @ Tue, 27 Jun 2017 16:25:17: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:17: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:17: #1 total tags in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:17: #1 tags after filtering in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:17: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:18: #2 number of paired peaks: 52 WARNING @ Tue, 27 Jun 2017 16:25:18: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:18: Process for pairing-model is terminated! cat: SRX113304.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113304.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:25:20: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:20: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:20: #1 total tags in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:20: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:20: #1 tags after filtering in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:20: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:20: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:21: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:21: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:21: #1 total tags in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:21: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:21: #1 tags after filtering in treatment: 15679943 INFO @ Tue, 27 Jun 2017 16:25:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:21: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:21: #2 number of paired peaks: 52 WARNING @ Tue, 27 Jun 2017 16:25:21: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:21: Process for pairing-model is terminated! cat: SRX113304.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113304.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:25:22: #2 number of paired peaks: 52 WARNING @ Tue, 27 Jun 2017 16:25:22: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:22: Process for pairing-model is terminated! cat: SRX113304.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113304.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113304.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。