Job ID = 9158333 sra ファイルのダウンロード中... Completed: 779802K bytes transferred in 8 seconds (741482K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2188064 spots for /home/okishinya/chipatlas/results/dm3/SRX113303/SRR392907.sra Written 2188064 spots total Written 20889019 spots for /home/okishinya/chipatlas/results/dm3/SRX113303/SRR392908.sra Written 20889019 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:13 23077083 reads; of these: 23077083 (100.00%) were unpaired; of these: 3206228 (13.89%) aligned 0 times 15755228 (68.27%) aligned exactly 1 time 4115627 (17.83%) aligned >1 times 86.11% overall alignment rate Time searching: 00:07:13 Overall time: 00:07:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4498847 / 19870855 = 0.2264 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:23:44: # Command line: callpeak -t SRX113303.bam -f BAM -g dm -n SRX113303.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113303.05 # format = BAM # ChIP-seq file = ['SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:44: # Command line: callpeak -t SRX113303.bam -f BAM -g dm -n SRX113303.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113303.10 # format = BAM # ChIP-seq file = ['SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:44: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:44: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:44: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:44: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:44: # Command line: callpeak -t SRX113303.bam -f BAM -g dm -n SRX113303.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113303.20 # format = BAM # ChIP-seq file = ['SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:44: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:44: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:52: 1000000 INFO @ Tue, 27 Jun 2017 16:23:52: 1000000 INFO @ Tue, 27 Jun 2017 16:23:53: 1000000 INFO @ Tue, 27 Jun 2017 16:23:59: 2000000 INFO @ Tue, 27 Jun 2017 16:24:00: 2000000 INFO @ Tue, 27 Jun 2017 16:24:01: 2000000 INFO @ Tue, 27 Jun 2017 16:24:07: 3000000 INFO @ Tue, 27 Jun 2017 16:24:07: 3000000 INFO @ Tue, 27 Jun 2017 16:24:10: 3000000 INFO @ Tue, 27 Jun 2017 16:24:14: 4000000 INFO @ Tue, 27 Jun 2017 16:24:15: 4000000 INFO @ Tue, 27 Jun 2017 16:24:19: 4000000 INFO @ Tue, 27 Jun 2017 16:24:22: 5000000 INFO @ Tue, 27 Jun 2017 16:24:23: 5000000 INFO @ Tue, 27 Jun 2017 16:24:27: 5000000 INFO @ Tue, 27 Jun 2017 16:24:29: 6000000 INFO @ Tue, 27 Jun 2017 16:24:30: 6000000 INFO @ Tue, 27 Jun 2017 16:24:36: 6000000 INFO @ Tue, 27 Jun 2017 16:24:37: 7000000 INFO @ Tue, 27 Jun 2017 16:24:38: 7000000 INFO @ Tue, 27 Jun 2017 16:24:44: 8000000 INFO @ Tue, 27 Jun 2017 16:24:45: 7000000 INFO @ Tue, 27 Jun 2017 16:24:45: 8000000 INFO @ Tue, 27 Jun 2017 16:24:52: 9000000 INFO @ Tue, 27 Jun 2017 16:24:53: 9000000 INFO @ Tue, 27 Jun 2017 16:24:53: 8000000 INFO @ Tue, 27 Jun 2017 16:24:59: 10000000 INFO @ Tue, 27 Jun 2017 16:25:01: 10000000 INFO @ Tue, 27 Jun 2017 16:25:02: 9000000 INFO @ Tue, 27 Jun 2017 16:25:07: 11000000 INFO @ Tue, 27 Jun 2017 16:25:08: 11000000 INFO @ Tue, 27 Jun 2017 16:25:10: 10000000 INFO @ Tue, 27 Jun 2017 16:25:14: 12000000 INFO @ Tue, 27 Jun 2017 16:25:16: 12000000 INFO @ Tue, 27 Jun 2017 16:25:19: 11000000 INFO @ Tue, 27 Jun 2017 16:25:22: 13000000 INFO @ Tue, 27 Jun 2017 16:25:23: 13000000 INFO @ Tue, 27 Jun 2017 16:25:28: 12000000 INFO @ Tue, 27 Jun 2017 16:25:30: 14000000 INFO @ Tue, 27 Jun 2017 16:25:31: 14000000 INFO @ Tue, 27 Jun 2017 16:25:36: 13000000 INFO @ Tue, 27 Jun 2017 16:25:37: 15000000 INFO @ Tue, 27 Jun 2017 16:25:39: 15000000 INFO @ Tue, 27 Jun 2017 16:25:41: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:41: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:41: #1 total tags in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:41: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:41: #1 tags after filtering in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:41: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:41: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:42: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:42: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:42: #1 total tags in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:42: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:42: #2 number of paired peaks: 61 WARNING @ Tue, 27 Jun 2017 16:25:42: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:42: Process for pairing-model is terminated! cat: SRX113303.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113303.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:25:42: #1 tags after filtering in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:42: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:42: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:43: #2 number of paired peaks: 61 WARNING @ Tue, 27 Jun 2017 16:25:43: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:43: Process for pairing-model is terminated! cat: SRX113303.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113303.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:25:44: 14000000 INFO @ Tue, 27 Jun 2017 16:25:52: 15000000 INFO @ Tue, 27 Jun 2017 16:25:54: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 16:25:54: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 16:25:54: #1 total tags in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:25:55: #1 tags after filtering in treatment: 15372008 INFO @ Tue, 27 Jun 2017 16:25:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:25:55: #1 finished! INFO @ Tue, 27 Jun 2017 16:25:55: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:25:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:25:56: #2 number of paired peaks: 61 WARNING @ Tue, 27 Jun 2017 16:25:56: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:25:56: Process for pairing-model is terminated! cat: SRX113303.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113303.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113303.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。