Job ID = 1293753 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,006,265 reads read : 8,012,530 reads written : 4,006,265 reads 0-length : 4,006,265 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 4006265 reads; of these: 4006265 (100.00%) were unpaired; of these: 261836 (6.54%) aligned 0 times 2997641 (74.82%) aligned exactly 1 time 746788 (18.64%) aligned >1 times 93.46% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 593645 / 3744429 = 0.1585 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:13:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:13:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:13:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:13:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:13:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:13:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:13:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:13:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:13:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:13:38: 1000000 INFO @ Mon, 03 Jun 2019 02:13:39: 1000000 INFO @ Mon, 03 Jun 2019 02:13:40: 1000000 INFO @ Mon, 03 Jun 2019 02:13:45: 2000000 INFO @ Mon, 03 Jun 2019 02:13:47: 2000000 INFO @ Mon, 03 Jun 2019 02:13:49: 2000000 INFO @ Mon, 03 Jun 2019 02:13:52: 3000000 INFO @ Mon, 03 Jun 2019 02:13:53: #1 tag size is determined as 60 bps INFO @ Mon, 03 Jun 2019 02:13:53: #1 tag size = 60 INFO @ Mon, 03 Jun 2019 02:13:53: #1 total tags in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:13:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:13:53: #1 tags after filtering in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:13:53: #1 finished! INFO @ Mon, 03 Jun 2019 02:13:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:13:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:13:53: #2 number of paired peaks: 101 WARNING @ Mon, 03 Jun 2019 02:13:53: Fewer paired peaks (101) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 101 pairs to build model! INFO @ Mon, 03 Jun 2019 02:13:53: start model_add_line... INFO @ Mon, 03 Jun 2019 02:13:53: start X-correlation... INFO @ Mon, 03 Jun 2019 02:13:54: end of X-cor INFO @ Mon, 03 Jun 2019 02:13:54: #2 finished! INFO @ Mon, 03 Jun 2019 02:13:54: #2 predicted fragment length is 70 bps INFO @ Mon, 03 Jun 2019 02:13:54: #2 alternative fragment length(s) may be 4,70,162,214,313,455,527 bps INFO @ Mon, 03 Jun 2019 02:13:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20_model.r WARNING @ Mon, 03 Jun 2019 02:13:54: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 02:13:54: #2 You may need to consider one of the other alternative d(s): 4,70,162,214,313,455,527 WARNING @ Mon, 03 Jun 2019 02:13:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 02:13:54: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:13:54: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:13:56: 3000000 INFO @ Mon, 03 Jun 2019 02:13:57: #1 tag size is determined as 60 bps INFO @ Mon, 03 Jun 2019 02:13:57: #1 tag size = 60 INFO @ Mon, 03 Jun 2019 02:13:57: #1 total tags in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:13:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:13:57: #1 tags after filtering in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:13:57: #1 finished! INFO @ Mon, 03 Jun 2019 02:13:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:13:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:13:57: #2 number of paired peaks: 101 WARNING @ Mon, 03 Jun 2019 02:13:57: Fewer paired peaks (101) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 101 pairs to build model! INFO @ Mon, 03 Jun 2019 02:13:57: start model_add_line... INFO @ Mon, 03 Jun 2019 02:13:57: start X-correlation... INFO @ Mon, 03 Jun 2019 02:13:57: end of X-cor INFO @ Mon, 03 Jun 2019 02:13:57: #2 finished! INFO @ Mon, 03 Jun 2019 02:13:57: #2 predicted fragment length is 70 bps INFO @ Mon, 03 Jun 2019 02:13:57: #2 alternative fragment length(s) may be 4,70,162,214,313,455,527 bps INFO @ Mon, 03 Jun 2019 02:13:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10_model.r WARNING @ Mon, 03 Jun 2019 02:13:57: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 02:13:57: #2 You may need to consider one of the other alternative d(s): 4,70,162,214,313,455,527 WARNING @ Mon, 03 Jun 2019 02:13:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 02:13:57: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:13:57: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:13:58: 3000000 INFO @ Mon, 03 Jun 2019 02:13:59: #1 tag size is determined as 60 bps INFO @ Mon, 03 Jun 2019 02:13:59: #1 tag size = 60 INFO @ Mon, 03 Jun 2019 02:13:59: #1 total tags in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:13:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:13:59: #1 tags after filtering in treatment: 3150784 INFO @ Mon, 03 Jun 2019 02:13:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:13:59: #1 finished! INFO @ Mon, 03 Jun 2019 02:13:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:13:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:14:00: #2 number of paired peaks: 101 WARNING @ Mon, 03 Jun 2019 02:14:00: Fewer paired peaks (101) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 101 pairs to build model! INFO @ Mon, 03 Jun 2019 02:14:00: start model_add_line... INFO @ Mon, 03 Jun 2019 02:14:00: start X-correlation... INFO @ Mon, 03 Jun 2019 02:14:00: end of X-cor INFO @ Mon, 03 Jun 2019 02:14:00: #2 finished! INFO @ Mon, 03 Jun 2019 02:14:00: #2 predicted fragment length is 70 bps INFO @ Mon, 03 Jun 2019 02:14:00: #2 alternative fragment length(s) may be 4,70,162,214,313,455,527 bps INFO @ Mon, 03 Jun 2019 02:14:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05_model.r WARNING @ Mon, 03 Jun 2019 02:14:00: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 02:14:00: #2 You may need to consider one of the other alternative d(s): 4,70,162,214,313,455,527 WARNING @ Mon, 03 Jun 2019 02:14:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 02:14:00: #3 Call peaks... INFO @ Mon, 03 Jun 2019 02:14:00: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 02:14:03: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:14:07: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:14:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20_peaks.xls INFO @ Mon, 03 Jun 2019 02:14:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 02:14:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.20_summits.bed INFO @ Mon, 03 Jun 2019 02:14:08: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (13 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:14:09: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 02:14:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10_peaks.xls INFO @ Mon, 03 Jun 2019 02:14:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 02:14:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.10_summits.bed INFO @ Mon, 03 Jun 2019 02:14:12: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (50 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:14:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05_peaks.xls INFO @ Mon, 03 Jun 2019 02:14:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 02:14:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1120697/SRX1120697.05_summits.bed INFO @ Mon, 03 Jun 2019 02:14:14: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (96 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。