Job ID = 1840909 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,111,745 reads read : 25,111,745 reads written : 25,111,745 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:12 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 420926 (1.68%) aligned 0 times 20783117 (82.76%) aligned exactly 1 time 3907702 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:07:13 Overall time: 00:07:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5654861 / 24690819 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 18 Jun 2019 16:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 18 Jun 2019 16:15:15: #1 read tag files... INFO @ Tue, 18 Jun 2019 16:15:15: #1 read treatment tags... INFO @ Tue, 18 Jun 2019 16:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 18 Jun 2019 16:15:15: #1 read tag files... INFO @ Tue, 18 Jun 2019 16:15:15: #1 read treatment tags... INFO @ Tue, 18 Jun 2019 16:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 18 Jun 2019 16:15:15: #1 read tag files... INFO @ Tue, 18 Jun 2019 16:15:15: #1 read treatment tags... INFO @ Tue, 18 Jun 2019 16:15:23: 1000000 INFO @ Tue, 18 Jun 2019 16:15:26: 1000000 INFO @ Tue, 18 Jun 2019 16:15:26: 1000000 INFO @ Tue, 18 Jun 2019 16:15:31: 2000000 INFO @ Tue, 18 Jun 2019 16:15:36: 2000000 INFO @ Tue, 18 Jun 2019 16:15:36: 2000000 INFO @ Tue, 18 Jun 2019 16:15:39: 3000000 INFO @ Tue, 18 Jun 2019 16:15:46: 3000000 INFO @ Tue, 18 Jun 2019 16:15:47: 3000000 INFO @ Tue, 18 Jun 2019 16:15:47: 4000000 INFO @ Tue, 18 Jun 2019 16:15:56: 5000000 INFO @ Tue, 18 Jun 2019 16:15:57: 4000000 INFO @ Tue, 18 Jun 2019 16:15:57: 4000000 INFO @ Tue, 18 Jun 2019 16:16:04: 6000000 INFO @ Tue, 18 Jun 2019 16:16:05: 5000000 INFO @ Tue, 18 Jun 2019 16:16:06: 5000000 INFO @ Tue, 18 Jun 2019 16:16:12: 7000000 INFO @ Tue, 18 Jun 2019 16:16:15: 6000000 INFO @ Tue, 18 Jun 2019 16:16:16: 6000000 INFO @ Tue, 18 Jun 2019 16:16:20: 8000000 INFO @ Tue, 18 Jun 2019 16:16:25: 7000000 INFO @ Tue, 18 Jun 2019 16:16:26: 7000000 INFO @ Tue, 18 Jun 2019 16:16:28: 9000000 INFO @ Tue, 18 Jun 2019 16:16:34: 8000000 INFO @ Tue, 18 Jun 2019 16:16:35: 10000000 INFO @ Tue, 18 Jun 2019 16:16:35: 8000000 INFO @ Tue, 18 Jun 2019 16:16:43: 11000000 INFO @ Tue, 18 Jun 2019 16:16:44: 9000000 INFO @ Tue, 18 Jun 2019 16:16:45: 9000000 INFO @ Tue, 18 Jun 2019 16:16:51: 12000000 INFO @ Tue, 18 Jun 2019 16:16:54: 10000000 INFO @ Tue, 18 Jun 2019 16:16:55: 10000000 INFO @ Tue, 18 Jun 2019 16:16:59: 13000000 INFO @ Tue, 18 Jun 2019 16:17:04: 11000000 INFO @ Tue, 18 Jun 2019 16:17:06: 11000000 INFO @ Tue, 18 Jun 2019 16:17:06: 14000000 INFO @ Tue, 18 Jun 2019 16:17:14: 12000000 INFO @ Tue, 18 Jun 2019 16:17:14: 15000000 INFO @ Tue, 18 Jun 2019 16:17:15: 12000000 INFO @ Tue, 18 Jun 2019 16:17:23: 16000000 INFO @ Tue, 18 Jun 2019 16:17:24: 13000000 INFO @ Tue, 18 Jun 2019 16:17:26: 13000000 INFO @ Tue, 18 Jun 2019 16:17:31: 17000000 INFO @ Tue, 18 Jun 2019 16:17:34: 14000000 INFO @ Tue, 18 Jun 2019 16:17:36: 14000000 INFO @ Tue, 18 Jun 2019 16:17:39: 18000000 INFO @ Tue, 18 Jun 2019 16:17:44: 15000000 INFO @ Tue, 18 Jun 2019 16:17:46: 15000000 INFO @ Tue, 18 Jun 2019 16:17:47: 19000000 INFO @ Tue, 18 Jun 2019 16:17:47: #1 tag size is determined as 44 bps INFO @ Tue, 18 Jun 2019 16:17:47: #1 tag size = 44 INFO @ Tue, 18 Jun 2019 16:17:47: #1 total tags in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:17:47: #1 user defined the maximum tags... INFO @ Tue, 18 Jun 2019 16:17:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 18 Jun 2019 16:17:48: #1 tags after filtering in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:17:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 18 Jun 2019 16:17:48: #1 finished! INFO @ Tue, 18 Jun 2019 16:17:48: #2 Build Peak Model... INFO @ Tue, 18 Jun 2019 16:17:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 18 Jun 2019 16:17:49: #2 number of paired peaks: 7 WARNING @ Tue, 18 Jun 2019 16:17:49: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 18 Jun 2019 16:17:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 18 Jun 2019 16:17:53: 16000000 INFO @ Tue, 18 Jun 2019 16:17:56: 16000000 INFO @ Tue, 18 Jun 2019 16:18:03: 17000000 INFO @ Tue, 18 Jun 2019 16:18:06: 17000000 INFO @ Tue, 18 Jun 2019 16:18:13: 18000000 INFO @ Tue, 18 Jun 2019 16:18:16: 18000000 INFO @ Tue, 18 Jun 2019 16:18:22: 19000000 INFO @ Tue, 18 Jun 2019 16:18:23: #1 tag size is determined as 44 bps INFO @ Tue, 18 Jun 2019 16:18:23: #1 tag size = 44 INFO @ Tue, 18 Jun 2019 16:18:23: #1 total tags in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:18:23: #1 user defined the maximum tags... INFO @ Tue, 18 Jun 2019 16:18:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 18 Jun 2019 16:18:23: #1 tags after filtering in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:18:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 18 Jun 2019 16:18:23: #1 finished! INFO @ Tue, 18 Jun 2019 16:18:23: #2 Build Peak Model... INFO @ Tue, 18 Jun 2019 16:18:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 18 Jun 2019 16:18:25: #2 number of paired peaks: 7 WARNING @ Tue, 18 Jun 2019 16:18:25: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 18 Jun 2019 16:18:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 18 Jun 2019 16:18:26: 19000000 INFO @ Tue, 18 Jun 2019 16:18:26: #1 tag size is determined as 44 bps INFO @ Tue, 18 Jun 2019 16:18:26: #1 tag size = 44 INFO @ Tue, 18 Jun 2019 16:18:26: #1 total tags in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:18:26: #1 user defined the maximum tags... INFO @ Tue, 18 Jun 2019 16:18:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 18 Jun 2019 16:18:27: #1 tags after filtering in treatment: 19035958 INFO @ Tue, 18 Jun 2019 16:18:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 18 Jun 2019 16:18:27: #1 finished! INFO @ Tue, 18 Jun 2019 16:18:27: #2 Build Peak Model... INFO @ Tue, 18 Jun 2019 16:18:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 18 Jun 2019 16:18:28: #2 number of paired peaks: 7 WARNING @ Tue, 18 Jun 2019 16:18:28: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 18 Jun 2019 16:18:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111820/SRX111820.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。