Job ID = 1293734 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,830,139 reads read : 15,830,139 reads written : 15,830,139 spots read : 18,878,990 reads read : 18,878,990 reads written : 18,878,990 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:03 34709129 reads; of these: 34709129 (100.00%) were unpaired; of these: 13768434 (39.67%) aligned 0 times 15350924 (44.23%) aligned exactly 1 time 5589771 (16.10%) aligned >1 times 60.33% overall alignment rate Time searching: 00:11:03 Overall time: 00:11:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5161257 / 20940695 = 0.2465 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:26:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:26:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:26:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:26:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:26:38: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:26:38: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:26:46: 1000000 INFO @ Mon, 03 Jun 2019 02:26:47: 1000000 INFO @ Mon, 03 Jun 2019 02:26:48: 1000000 INFO @ Mon, 03 Jun 2019 02:26:53: 2000000 INFO @ Mon, 03 Jun 2019 02:26:55: 2000000 INFO @ Mon, 03 Jun 2019 02:26:57: 2000000 INFO @ Mon, 03 Jun 2019 02:27:00: 3000000 INFO @ Mon, 03 Jun 2019 02:27:03: 3000000 INFO @ Mon, 03 Jun 2019 02:27:06: 3000000 INFO @ Mon, 03 Jun 2019 02:27:08: 4000000 INFO @ Mon, 03 Jun 2019 02:27:12: 4000000 INFO @ Mon, 03 Jun 2019 02:27:15: 4000000 INFO @ Mon, 03 Jun 2019 02:27:16: 5000000 INFO @ Mon, 03 Jun 2019 02:27:20: 5000000 INFO @ Mon, 03 Jun 2019 02:27:23: 6000000 INFO @ Mon, 03 Jun 2019 02:27:24: 5000000 INFO @ Mon, 03 Jun 2019 02:27:29: 6000000 INFO @ Mon, 03 Jun 2019 02:27:30: 7000000 INFO @ Mon, 03 Jun 2019 02:27:33: 6000000 INFO @ Mon, 03 Jun 2019 02:27:37: 8000000 INFO @ Mon, 03 Jun 2019 02:27:38: 7000000 INFO @ Mon, 03 Jun 2019 02:27:42: 7000000 INFO @ Mon, 03 Jun 2019 02:27:45: 9000000 INFO @ Mon, 03 Jun 2019 02:27:46: 8000000 INFO @ Mon, 03 Jun 2019 02:27:50: 8000000 INFO @ Mon, 03 Jun 2019 02:27:52: 10000000 INFO @ Mon, 03 Jun 2019 02:27:55: 9000000 INFO @ Mon, 03 Jun 2019 02:27:59: 11000000 INFO @ Mon, 03 Jun 2019 02:27:59: 9000000 INFO @ Mon, 03 Jun 2019 02:28:03: 10000000 INFO @ Mon, 03 Jun 2019 02:28:07: 12000000 INFO @ Mon, 03 Jun 2019 02:28:08: 10000000 INFO @ Mon, 03 Jun 2019 02:28:12: 11000000 INFO @ Mon, 03 Jun 2019 02:28:14: 13000000 INFO @ Mon, 03 Jun 2019 02:28:17: 11000000 INFO @ Mon, 03 Jun 2019 02:28:20: 12000000 INFO @ Mon, 03 Jun 2019 02:28:22: 14000000 INFO @ Mon, 03 Jun 2019 02:28:26: 12000000 INFO @ Mon, 03 Jun 2019 02:28:29: 13000000 INFO @ Mon, 03 Jun 2019 02:28:29: 15000000 INFO @ Mon, 03 Jun 2019 02:28:35: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:28:35: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:28:35: #1 total tags in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:28:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:28:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:28:35: 13000000 INFO @ Mon, 03 Jun 2019 02:28:36: #1 tags after filtering in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:28:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:28:36: #1 finished! INFO @ Mon, 03 Jun 2019 02:28:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:28:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:28:37: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:28:37: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:28:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:28:38: 14000000 INFO @ Mon, 03 Jun 2019 02:28:44: 14000000 INFO @ Mon, 03 Jun 2019 02:28:46: 15000000 INFO @ Mon, 03 Jun 2019 02:28:53: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:28:53: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:28:53: #1 total tags in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:28:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:28:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:28:53: 15000000 INFO @ Mon, 03 Jun 2019 02:28:53: #1 tags after filtering in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:28:53: #1 finished! INFO @ Mon, 03 Jun 2019 02:28:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:28:55: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:28:55: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:28:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:29:00: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:29:00: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:29:00: #1 total tags in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:29:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:29:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:29:00: #1 tags after filtering in treatment: 15779438 INFO @ Mon, 03 Jun 2019 02:29:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:29:00: #1 finished! INFO @ Mon, 03 Jun 2019 02:29:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:29:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:29:02: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:29:02: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:29:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111815/SRX111815.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。