Job ID = 9158320 sra ファイルのダウンロード中... Completed: 554719K bytes transferred in 8 seconds (567373K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18189117 spots for /home/okishinya/chipatlas/results/dm3/SRX111809/SRR390270.sra Written 18189117 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:31 18189117 reads; of these: 18189117 (100.00%) were unpaired; of these: 702845 (3.86%) aligned 0 times 12502599 (68.74%) aligned exactly 1 time 4983673 (27.40%) aligned >1 times 96.14% overall alignment rate Time searching: 00:06:31 Overall time: 00:06:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3084557 / 17486272 = 0.1764 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:13:27: # Command line: callpeak -t SRX111809.bam -f BAM -g dm -n SRX111809.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111809.10 # format = BAM # ChIP-seq file = ['SRX111809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:13:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:13:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:13:27: # Command line: callpeak -t SRX111809.bam -f BAM -g dm -n SRX111809.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111809.05 # format = BAM # ChIP-seq file = ['SRX111809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:13:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:13:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:13:27: # Command line: callpeak -t SRX111809.bam -f BAM -g dm -n SRX111809.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111809.20 # format = BAM # ChIP-seq file = ['SRX111809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:13:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:13:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:13:34: 1000000 INFO @ Tue, 27 Jun 2017 16:13:34: 1000000 INFO @ Tue, 27 Jun 2017 16:13:34: 1000000 INFO @ Tue, 27 Jun 2017 16:13:41: 2000000 INFO @ Tue, 27 Jun 2017 16:13:41: 2000000 INFO @ Tue, 27 Jun 2017 16:13:41: 2000000 INFO @ Tue, 27 Jun 2017 16:13:47: 3000000 INFO @ Tue, 27 Jun 2017 16:13:47: 3000000 INFO @ Tue, 27 Jun 2017 16:13:47: 3000000 INFO @ Tue, 27 Jun 2017 16:13:54: 4000000 INFO @ Tue, 27 Jun 2017 16:13:54: 4000000 INFO @ Tue, 27 Jun 2017 16:13:54: 4000000 INFO @ Tue, 27 Jun 2017 16:14:01: 5000000 INFO @ Tue, 27 Jun 2017 16:14:01: 5000000 INFO @ Tue, 27 Jun 2017 16:14:01: 5000000 INFO @ Tue, 27 Jun 2017 16:14:07: 6000000 INFO @ Tue, 27 Jun 2017 16:14:08: 6000000 INFO @ Tue, 27 Jun 2017 16:14:08: 6000000 INFO @ Tue, 27 Jun 2017 16:14:14: 7000000 INFO @ Tue, 27 Jun 2017 16:14:15: 7000000 INFO @ Tue, 27 Jun 2017 16:14:15: 7000000 INFO @ Tue, 27 Jun 2017 16:14:20: 8000000 INFO @ Tue, 27 Jun 2017 16:14:22: 8000000 INFO @ Tue, 27 Jun 2017 16:14:22: 8000000 INFO @ Tue, 27 Jun 2017 16:14:27: 9000000 INFO @ Tue, 27 Jun 2017 16:14:29: 9000000 INFO @ Tue, 27 Jun 2017 16:14:29: 9000000 INFO @ Tue, 27 Jun 2017 16:14:33: 10000000 INFO @ Tue, 27 Jun 2017 16:14:36: 10000000 INFO @ Tue, 27 Jun 2017 16:14:36: 10000000 INFO @ Tue, 27 Jun 2017 16:14:40: 11000000 INFO @ Tue, 27 Jun 2017 16:14:44: 11000000 INFO @ Tue, 27 Jun 2017 16:14:44: 11000000 INFO @ Tue, 27 Jun 2017 16:14:47: 12000000 INFO @ Tue, 27 Jun 2017 16:14:52: 12000000 INFO @ Tue, 27 Jun 2017 16:14:52: 12000000 INFO @ Tue, 27 Jun 2017 16:14:53: 13000000 INFO @ Tue, 27 Jun 2017 16:14:59: 13000000 INFO @ Tue, 27 Jun 2017 16:14:59: 13000000 INFO @ Tue, 27 Jun 2017 16:15:00: 14000000 INFO @ Tue, 27 Jun 2017 16:15:02: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:15:02: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:15:02: #1 total tags in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:15:03: #1 tags after filtering in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:15:03: #1 finished! INFO @ Tue, 27 Jun 2017 16:15:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:15:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:15:04: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:15:04: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:15:04: Process for pairing-model is terminated! cat: SRX111809.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111809.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:15:08: 14000000 INFO @ Tue, 27 Jun 2017 16:15:08: 14000000 INFO @ Tue, 27 Jun 2017 16:15:11: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:15:11: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:15:11: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:15:11: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:15:11: #1 total tags in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:11: #1 total tags in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:11: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:15:11: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:15:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:15:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:15:11: #1 tags after filtering in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:11: #1 tags after filtering in treatment: 14401715 INFO @ Tue, 27 Jun 2017 16:15:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:15:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:15:11: #1 finished! INFO @ Tue, 27 Jun 2017 16:15:11: #1 finished! INFO @ Tue, 27 Jun 2017 16:15:11: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:15:11: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:15:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:15:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:15:12: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:15:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:15:12: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 16:15:12: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:15:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:15:12: Process for pairing-model is terminated! cat: SRX111809.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX111809.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111809.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111809.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111809.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。