Job ID = 9158319 sra ファイルのダウンロード中... Completed: 1080788K bytes transferred in 10 seconds (830783K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 16715588 spots for /home/okishinya/chipatlas/results/dm3/SRX111808/SRR390269.sra Written 16715588 spots total Written 20354253 spots for /home/okishinya/chipatlas/results/dm3/SRX111808/SRR390268.sra Written 20354253 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:59 37069841 reads; of these: 37069841 (100.00%) were unpaired; of these: 2142100 (5.78%) aligned 0 times 24864604 (67.08%) aligned exactly 1 time 10063137 (27.15%) aligned >1 times 94.22% overall alignment rate Time searching: 00:12:59 Overall time: 00:12:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11419759 / 34927741 = 0.3270 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:23:50: # Command line: callpeak -t SRX111808.bam -f BAM -g dm -n SRX111808.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111808.05 # format = BAM # ChIP-seq file = ['SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:50: # Command line: callpeak -t SRX111808.bam -f BAM -g dm -n SRX111808.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111808.20 # format = BAM # ChIP-seq file = ['SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:50: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:50: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:50: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:50: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:50: # Command line: callpeak -t SRX111808.bam -f BAM -g dm -n SRX111808.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111808.10 # format = BAM # ChIP-seq file = ['SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:23:50: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:23:50: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:23:56: 1000000 INFO @ Tue, 27 Jun 2017 16:23:56: 1000000 INFO @ Tue, 27 Jun 2017 16:23:56: 1000000 INFO @ Tue, 27 Jun 2017 16:24:02: 2000000 INFO @ Tue, 27 Jun 2017 16:24:02: 2000000 INFO @ Tue, 27 Jun 2017 16:24:03: 2000000 INFO @ Tue, 27 Jun 2017 16:24:09: 3000000 INFO @ Tue, 27 Jun 2017 16:24:09: 3000000 INFO @ Tue, 27 Jun 2017 16:24:09: 3000000 INFO @ Tue, 27 Jun 2017 16:24:16: 4000000 INFO @ Tue, 27 Jun 2017 16:24:16: 4000000 INFO @ Tue, 27 Jun 2017 16:24:16: 4000000 INFO @ Tue, 27 Jun 2017 16:24:23: 5000000 INFO @ Tue, 27 Jun 2017 16:24:23: 5000000 INFO @ Tue, 27 Jun 2017 16:24:24: 5000000 INFO @ Tue, 27 Jun 2017 16:24:30: 6000000 INFO @ Tue, 27 Jun 2017 16:24:30: 6000000 INFO @ Tue, 27 Jun 2017 16:24:31: 6000000 INFO @ Tue, 27 Jun 2017 16:24:37: 7000000 INFO @ Tue, 27 Jun 2017 16:24:37: 7000000 INFO @ Tue, 27 Jun 2017 16:24:39: 7000000 INFO @ Tue, 27 Jun 2017 16:24:45: 8000000 INFO @ Tue, 27 Jun 2017 16:24:45: 8000000 INFO @ Tue, 27 Jun 2017 16:24:47: 8000000 INFO @ Tue, 27 Jun 2017 16:24:52: 9000000 INFO @ Tue, 27 Jun 2017 16:24:52: 9000000 INFO @ Tue, 27 Jun 2017 16:24:54: 9000000 INFO @ Tue, 27 Jun 2017 16:24:59: 10000000 INFO @ Tue, 27 Jun 2017 16:24:59: 10000000 INFO @ Tue, 27 Jun 2017 16:25:02: 10000000 INFO @ Tue, 27 Jun 2017 16:25:07: 11000000 INFO @ Tue, 27 Jun 2017 16:25:07: 11000000 INFO @ Tue, 27 Jun 2017 16:25:09: 11000000 INFO @ Tue, 27 Jun 2017 16:25:14: 12000000 INFO @ Tue, 27 Jun 2017 16:25:14: 12000000 INFO @ Tue, 27 Jun 2017 16:25:17: 12000000 INFO @ Tue, 27 Jun 2017 16:25:21: 13000000 INFO @ Tue, 27 Jun 2017 16:25:21: 13000000 INFO @ Tue, 27 Jun 2017 16:25:25: 13000000 INFO @ Tue, 27 Jun 2017 16:25:29: 14000000 INFO @ Tue, 27 Jun 2017 16:25:29: 14000000 INFO @ Tue, 27 Jun 2017 16:25:32: 14000000 INFO @ Tue, 27 Jun 2017 16:25:36: 15000000 INFO @ Tue, 27 Jun 2017 16:25:36: 15000000 INFO @ Tue, 27 Jun 2017 16:25:40: 15000000 INFO @ Tue, 27 Jun 2017 16:25:43: 16000000 INFO @ Tue, 27 Jun 2017 16:25:43: 16000000 INFO @ Tue, 27 Jun 2017 16:25:48: 16000000 INFO @ Tue, 27 Jun 2017 16:25:51: 17000000 INFO @ Tue, 27 Jun 2017 16:25:51: 17000000 INFO @ Tue, 27 Jun 2017 16:25:55: 17000000 INFO @ Tue, 27 Jun 2017 16:25:58: 18000000 INFO @ Tue, 27 Jun 2017 16:25:58: 18000000 INFO @ Tue, 27 Jun 2017 16:26:03: 18000000 INFO @ Tue, 27 Jun 2017 16:26:06: 19000000 INFO @ Tue, 27 Jun 2017 16:26:06: 19000000 INFO @ Tue, 27 Jun 2017 16:26:11: 19000000 INFO @ Tue, 27 Jun 2017 16:26:13: 20000000 INFO @ Tue, 27 Jun 2017 16:26:13: 20000000 INFO @ Tue, 27 Jun 2017 16:26:19: 20000000 INFO @ Tue, 27 Jun 2017 16:26:20: 21000000 INFO @ Tue, 27 Jun 2017 16:26:20: 21000000 INFO @ Tue, 27 Jun 2017 16:26:26: 21000000 INFO @ Tue, 27 Jun 2017 16:26:27: 22000000 INFO @ Tue, 27 Jun 2017 16:26:27: 22000000 INFO @ Tue, 27 Jun 2017 16:26:34: 22000000 INFO @ Tue, 27 Jun 2017 16:26:34: 23000000 INFO @ Tue, 27 Jun 2017 16:26:34: 23000000 INFO @ Tue, 27 Jun 2017 16:26:38: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:26:38: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:26:38: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:26:38: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:26:38: #1 total tags in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:38: #1 total tags in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:38: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:26:38: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:26:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:26:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:26:38: #1 tags after filtering in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:26:38: #1 finished! INFO @ Tue, 27 Jun 2017 16:26:38: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:26:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:26:38: #1 tags after filtering in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:26:38: #1 finished! INFO @ Tue, 27 Jun 2017 16:26:38: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:26:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:26:40: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 16:26:40: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:26:40: Process for pairing-model is terminated! cat: SRX111808.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111808.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:26:40: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 16:26:40: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:26:40: Process for pairing-model is terminated! cat: SRX111808.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111808.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:26:41: 23000000 INFO @ Tue, 27 Jun 2017 16:26:45: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:26:45: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:26:45: #1 total tags in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:45: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:26:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:26:45: #1 tags after filtering in treatment: 23507982 INFO @ Tue, 27 Jun 2017 16:26:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:26:45: #1 finished! INFO @ Tue, 27 Jun 2017 16:26:45: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:26:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:26:47: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 16:26:47: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:26:47: Process for pairing-model is terminated! cat: SRX111808.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111808.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111808.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。