Job ID = 9158317 sra ファイルのダウンロード中... Completed: 530512K bytes transferred in 7 seconds (573795K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18671221 spots for /home/okishinya/chipatlas/results/dm3/SRX111806/SRR390265.sra Written 18671221 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:01 18671221 reads; of these: 18671221 (100.00%) were unpaired; of these: 277187 (1.48%) aligned 0 times 15578154 (83.43%) aligned exactly 1 time 2815880 (15.08%) aligned >1 times 98.52% overall alignment rate Time searching: 00:05:01 Overall time: 00:05:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3187782 / 18394034 = 0.1733 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:09:46: # Command line: callpeak -t SRX111806.bam -f BAM -g dm -n SRX111806.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111806.05 # format = BAM # ChIP-seq file = ['SRX111806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:09:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:09:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:09:46: # Command line: callpeak -t SRX111806.bam -f BAM -g dm -n SRX111806.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111806.20 # format = BAM # ChIP-seq file = ['SRX111806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:09:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:09:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:09:46: # Command line: callpeak -t SRX111806.bam -f BAM -g dm -n SRX111806.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111806.10 # format = BAM # ChIP-seq file = ['SRX111806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:09:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:09:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:09:52: 1000000 INFO @ Tue, 27 Jun 2017 16:09:52: 1000000 INFO @ Tue, 27 Jun 2017 16:09:52: 1000000 INFO @ Tue, 27 Jun 2017 16:09:58: 2000000 INFO @ Tue, 27 Jun 2017 16:09:58: 2000000 INFO @ Tue, 27 Jun 2017 16:09:59: 2000000 INFO @ Tue, 27 Jun 2017 16:10:05: 3000000 INFO @ Tue, 27 Jun 2017 16:10:05: 3000000 INFO @ Tue, 27 Jun 2017 16:10:05: 3000000 INFO @ Tue, 27 Jun 2017 16:10:11: 4000000 INFO @ Tue, 27 Jun 2017 16:10:11: 4000000 INFO @ Tue, 27 Jun 2017 16:10:11: 4000000 INFO @ Tue, 27 Jun 2017 16:10:17: 5000000 INFO @ Tue, 27 Jun 2017 16:10:17: 5000000 INFO @ Tue, 27 Jun 2017 16:10:17: 5000000 INFO @ Tue, 27 Jun 2017 16:10:23: 6000000 INFO @ Tue, 27 Jun 2017 16:10:23: 6000000 INFO @ Tue, 27 Jun 2017 16:10:23: 6000000 INFO @ Tue, 27 Jun 2017 16:10:29: 7000000 INFO @ Tue, 27 Jun 2017 16:10:30: 7000000 INFO @ Tue, 27 Jun 2017 16:10:30: 7000000 INFO @ Tue, 27 Jun 2017 16:10:35: 8000000 INFO @ Tue, 27 Jun 2017 16:10:36: 8000000 INFO @ Tue, 27 Jun 2017 16:10:36: 8000000 INFO @ Tue, 27 Jun 2017 16:10:42: 9000000 INFO @ Tue, 27 Jun 2017 16:10:42: 9000000 INFO @ Tue, 27 Jun 2017 16:10:42: 9000000 INFO @ Tue, 27 Jun 2017 16:10:48: 10000000 INFO @ Tue, 27 Jun 2017 16:10:48: 10000000 INFO @ Tue, 27 Jun 2017 16:10:49: 10000000 INFO @ Tue, 27 Jun 2017 16:10:54: 11000000 INFO @ Tue, 27 Jun 2017 16:10:54: 11000000 INFO @ Tue, 27 Jun 2017 16:10:55: 11000000 INFO @ Tue, 27 Jun 2017 16:11:00: 12000000 INFO @ Tue, 27 Jun 2017 16:11:01: 12000000 INFO @ Tue, 27 Jun 2017 16:11:01: 12000000 INFO @ Tue, 27 Jun 2017 16:11:06: 13000000 INFO @ Tue, 27 Jun 2017 16:11:08: 13000000 INFO @ Tue, 27 Jun 2017 16:11:08: 13000000 INFO @ Tue, 27 Jun 2017 16:11:13: 14000000 INFO @ Tue, 27 Jun 2017 16:11:15: 14000000 INFO @ Tue, 27 Jun 2017 16:11:15: 14000000 INFO @ Tue, 27 Jun 2017 16:11:19: 15000000 INFO @ Tue, 27 Jun 2017 16:11:20: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:11:20: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:11:20: #1 total tags in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:20: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:11:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:11:21: #1 tags after filtering in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:11:21: #1 finished! INFO @ Tue, 27 Jun 2017 16:11:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:11:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:11:21: 15000000 INFO @ Tue, 27 Jun 2017 16:11:22: #2 number of paired peaks: 42 WARNING @ Tue, 27 Jun 2017 16:11:22: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:11:22: Process for pairing-model is terminated! cat: SRX111806.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111806.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:11:22: 15000000 INFO @ Tue, 27 Jun 2017 16:11:23: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:11:23: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:11:23: #1 total tags in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:23: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:11:23: #1 tags after filtering in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:11:23: #1 finished! INFO @ Tue, 27 Jun 2017 16:11:23: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:11:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:11:23: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:11:23: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:11:23: #1 total tags in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:23: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:11:24: #1 tags after filtering in treatment: 15206252 INFO @ Tue, 27 Jun 2017 16:11:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:11:24: #1 finished! INFO @ Tue, 27 Jun 2017 16:11:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:11:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:11:24: #2 number of paired peaks: 42 WARNING @ Tue, 27 Jun 2017 16:11:24: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:11:24: Process for pairing-model is terminated! cat: SRX111806.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111806.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:11:25: #2 number of paired peaks: 42 WARNING @ Tue, 27 Jun 2017 16:11:25: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:11:25: Process for pairing-model is terminated! cat: SRX111806.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111806.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111806.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。