Job ID = 9158305 sra ファイルのダウンロード中... Completed: 1630164K bytes transferred in 16 seconds (832780K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24689576 spots for /home/okishinya/chipatlas/results/dm3/SRX111803/SRR390260.sra Written 24689576 spots total Written 29844647 spots for /home/okishinya/chipatlas/results/dm3/SRX111803/SRR390261.sra Written 29844647 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:05 54534223 reads; of these: 54534223 (100.00%) were unpaired; of these: 1390780 (2.55%) aligned 0 times 39951129 (73.26%) aligned exactly 1 time 13192314 (24.19%) aligned >1 times 97.45% overall alignment rate Time searching: 00:20:05 Overall time: 00:20:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20316486 / 53143443 = 0.3823 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:32:33: # Command line: callpeak -t SRX111803.bam -f BAM -g dm -n SRX111803.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111803.05 # format = BAM # ChIP-seq file = ['SRX111803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:32:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:32:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:32:33: # Command line: callpeak -t SRX111803.bam -f BAM -g dm -n SRX111803.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111803.10 # format = BAM # ChIP-seq file = ['SRX111803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:32:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:32:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:32:33: # Command line: callpeak -t SRX111803.bam -f BAM -g dm -n SRX111803.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111803.20 # format = BAM # ChIP-seq file = ['SRX111803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:32:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:32:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:32:42: 1000000 INFO @ Tue, 27 Jun 2017 16:32:42: 1000000 INFO @ Tue, 27 Jun 2017 16:32:43: 1000000 INFO @ Tue, 27 Jun 2017 16:32:50: 2000000 INFO @ Tue, 27 Jun 2017 16:32:52: 2000000 INFO @ Tue, 27 Jun 2017 16:32:52: 2000000 INFO @ Tue, 27 Jun 2017 16:32:59: 3000000 INFO @ Tue, 27 Jun 2017 16:33:01: 3000000 INFO @ Tue, 27 Jun 2017 16:33:02: 3000000 INFO @ Tue, 27 Jun 2017 16:33:08: 4000000 INFO @ Tue, 27 Jun 2017 16:33:10: 4000000 INFO @ Tue, 27 Jun 2017 16:33:12: 4000000 INFO @ Tue, 27 Jun 2017 16:33:17: 5000000 INFO @ Tue, 27 Jun 2017 16:33:19: 5000000 INFO @ Tue, 27 Jun 2017 16:33:21: 5000000 INFO @ Tue, 27 Jun 2017 16:33:26: 6000000 INFO @ Tue, 27 Jun 2017 16:33:28: 6000000 INFO @ Tue, 27 Jun 2017 16:33:31: 6000000 INFO @ Tue, 27 Jun 2017 16:33:35: 7000000 INFO @ Tue, 27 Jun 2017 16:33:37: 7000000 INFO @ Tue, 27 Jun 2017 16:33:41: 7000000 INFO @ Tue, 27 Jun 2017 16:33:44: 8000000 INFO @ Tue, 27 Jun 2017 16:33:46: 8000000 INFO @ Tue, 27 Jun 2017 16:33:51: 8000000 INFO @ Tue, 27 Jun 2017 16:33:53: 9000000 INFO @ Tue, 27 Jun 2017 16:33:55: 9000000 INFO @ Tue, 27 Jun 2017 16:34:01: 9000000 INFO @ Tue, 27 Jun 2017 16:34:02: 10000000 INFO @ Tue, 27 Jun 2017 16:34:04: 10000000 INFO @ Tue, 27 Jun 2017 16:34:11: 10000000 INFO @ Tue, 27 Jun 2017 16:34:11: 11000000 INFO @ Tue, 27 Jun 2017 16:34:13: 11000000 INFO @ Tue, 27 Jun 2017 16:34:20: 12000000 INFO @ Tue, 27 Jun 2017 16:34:20: 11000000 INFO @ Tue, 27 Jun 2017 16:34:22: 12000000 INFO @ Tue, 27 Jun 2017 16:34:29: 13000000 INFO @ Tue, 27 Jun 2017 16:34:30: 12000000 INFO @ Tue, 27 Jun 2017 16:34:30: 13000000 INFO @ Tue, 27 Jun 2017 16:34:38: 14000000 INFO @ Tue, 27 Jun 2017 16:34:39: 14000000 INFO @ Tue, 27 Jun 2017 16:34:40: 13000000 INFO @ Tue, 27 Jun 2017 16:34:47: 15000000 INFO @ Tue, 27 Jun 2017 16:34:48: 15000000 INFO @ Tue, 27 Jun 2017 16:34:50: 14000000 INFO @ Tue, 27 Jun 2017 16:34:56: 16000000 INFO @ Tue, 27 Jun 2017 16:34:57: 16000000 INFO @ Tue, 27 Jun 2017 16:35:00: 15000000 INFO @ Tue, 27 Jun 2017 16:35:04: 17000000 INFO @ Tue, 27 Jun 2017 16:35:07: 17000000 INFO @ Tue, 27 Jun 2017 16:35:09: 16000000 INFO @ Tue, 27 Jun 2017 16:35:13: 18000000 INFO @ Tue, 27 Jun 2017 16:35:17: 18000000 INFO @ Tue, 27 Jun 2017 16:35:18: 17000000 INFO @ Tue, 27 Jun 2017 16:35:22: 19000000 INFO @ Tue, 27 Jun 2017 16:35:27: 18000000 INFO @ Tue, 27 Jun 2017 16:35:27: 19000000 INFO @ Tue, 27 Jun 2017 16:35:31: 20000000 INFO @ Tue, 27 Jun 2017 16:35:36: 19000000 INFO @ Tue, 27 Jun 2017 16:35:37: 20000000 INFO @ Tue, 27 Jun 2017 16:35:40: 21000000 INFO @ Tue, 27 Jun 2017 16:35:45: 20000000 INFO @ Tue, 27 Jun 2017 16:35:47: 21000000 INFO @ Tue, 27 Jun 2017 16:35:49: 22000000 INFO @ Tue, 27 Jun 2017 16:35:54: 21000000 INFO @ Tue, 27 Jun 2017 16:35:57: 22000000 INFO @ Tue, 27 Jun 2017 16:35:58: 23000000 INFO @ Tue, 27 Jun 2017 16:36:03: 22000000 INFO @ Tue, 27 Jun 2017 16:36:07: 23000000 INFO @ Tue, 27 Jun 2017 16:36:07: 24000000 INFO @ Tue, 27 Jun 2017 16:36:12: 23000000 INFO @ Tue, 27 Jun 2017 16:36:16: 25000000 INFO @ Tue, 27 Jun 2017 16:36:17: 24000000 INFO @ Tue, 27 Jun 2017 16:36:21: 24000000 INFO @ Tue, 27 Jun 2017 16:36:25: 26000000 INFO @ Tue, 27 Jun 2017 16:36:27: 25000000 INFO @ Tue, 27 Jun 2017 16:36:30: 25000000 INFO @ Tue, 27 Jun 2017 16:36:34: 27000000 INFO @ Tue, 27 Jun 2017 16:36:36: 26000000 INFO @ Tue, 27 Jun 2017 16:36:38: 26000000 INFO @ Tue, 27 Jun 2017 16:36:43: 28000000 INFO @ Tue, 27 Jun 2017 16:36:45: 27000000 INFO @ Tue, 27 Jun 2017 16:36:46: 27000000 INFO @ Tue, 27 Jun 2017 16:36:52: 29000000 INFO @ Tue, 27 Jun 2017 16:36:53: 28000000 INFO @ Tue, 27 Jun 2017 16:36:55: 28000000 INFO @ Tue, 27 Jun 2017 16:37:00: 29000000 INFO @ Tue, 27 Jun 2017 16:37:00: 30000000 INFO @ Tue, 27 Jun 2017 16:37:05: 29000000 INFO @ Tue, 27 Jun 2017 16:37:08: 30000000 INFO @ Tue, 27 Jun 2017 16:37:09: 31000000 INFO @ Tue, 27 Jun 2017 16:37:14: 30000000 INFO @ Tue, 27 Jun 2017 16:37:15: 31000000 INFO @ Tue, 27 Jun 2017 16:37:18: 32000000 INFO @ Tue, 27 Jun 2017 16:37:23: 32000000 INFO @ Tue, 27 Jun 2017 16:37:24: 31000000 INFO @ Tue, 27 Jun 2017 16:37:25: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:25: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:25: #1 total tags in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:25: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:26: #1 tags after filtering in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:26: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:26: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:28: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:37:28: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:28: Process for pairing-model is terminated! cat: SRX111803.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111803.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:37:30: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:30: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:30: #1 total tags in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:30: #1 tags after filtering in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:30: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:32: 32000000 INFO @ Tue, 27 Jun 2017 16:37:33: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:37:33: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:33: Process for pairing-model is terminated! cat: SRX111803.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111803.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:37:39: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:37:39: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:37:39: #1 total tags in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:39: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:37:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:37:40: #1 tags after filtering in treatment: 32826957 INFO @ Tue, 27 Jun 2017 16:37:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:37:40: #1 finished! INFO @ Tue, 27 Jun 2017 16:37:40: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:37:42: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:37:42: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:37:42: Process for pairing-model is terminated! cat: SRX111803.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111803.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111803.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。