Job ID = 1293722 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,927,623 reads read : 19,927,623 reads written : 19,927,623 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:15 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 222057 (1.11%) aligned 0 times 14963188 (75.09%) aligned exactly 1 time 4742378 (23.80%) aligned >1 times 98.89% overall alignment rate Time searching: 00:07:15 Overall time: 00:07:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4260903 / 19705566 = 0.2162 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:05:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:05:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:05:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:05:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:05:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:05:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:05:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:05:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:05:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:05:22: 1000000 INFO @ Mon, 03 Jun 2019 02:05:23: 1000000 INFO @ Mon, 03 Jun 2019 02:05:23: 1000000 INFO @ Mon, 03 Jun 2019 02:05:30: 2000000 INFO @ Mon, 03 Jun 2019 02:05:30: 2000000 INFO @ Mon, 03 Jun 2019 02:05:31: 2000000 INFO @ Mon, 03 Jun 2019 02:05:37: 3000000 INFO @ Mon, 03 Jun 2019 02:05:37: 3000000 INFO @ Mon, 03 Jun 2019 02:05:39: 3000000 INFO @ Mon, 03 Jun 2019 02:05:44: 4000000 INFO @ Mon, 03 Jun 2019 02:05:44: 4000000 INFO @ Mon, 03 Jun 2019 02:05:47: 4000000 INFO @ Mon, 03 Jun 2019 02:05:52: 5000000 INFO @ Mon, 03 Jun 2019 02:05:52: 5000000 INFO @ Mon, 03 Jun 2019 02:05:55: 5000000 INFO @ Mon, 03 Jun 2019 02:05:59: 6000000 INFO @ Mon, 03 Jun 2019 02:05:59: 6000000 INFO @ Mon, 03 Jun 2019 02:06:03: 6000000 INFO @ Mon, 03 Jun 2019 02:06:06: 7000000 INFO @ Mon, 03 Jun 2019 02:06:07: 7000000 INFO @ Mon, 03 Jun 2019 02:06:11: 7000000 INFO @ Mon, 03 Jun 2019 02:06:14: 8000000 INFO @ Mon, 03 Jun 2019 02:06:14: 8000000 INFO @ Mon, 03 Jun 2019 02:06:19: 8000000 INFO @ Mon, 03 Jun 2019 02:06:21: 9000000 INFO @ Mon, 03 Jun 2019 02:06:21: 9000000 INFO @ Mon, 03 Jun 2019 02:06:27: 9000000 INFO @ Mon, 03 Jun 2019 02:06:28: 10000000 INFO @ Mon, 03 Jun 2019 02:06:29: 10000000 INFO @ Mon, 03 Jun 2019 02:06:35: 10000000 INFO @ Mon, 03 Jun 2019 02:06:36: 11000000 INFO @ Mon, 03 Jun 2019 02:06:36: 11000000 INFO @ Mon, 03 Jun 2019 02:06:44: 11000000 INFO @ Mon, 03 Jun 2019 02:06:44: 12000000 INFO @ Mon, 03 Jun 2019 02:06:44: 12000000 INFO @ Mon, 03 Jun 2019 02:06:51: 13000000 INFO @ Mon, 03 Jun 2019 02:06:52: 13000000 INFO @ Mon, 03 Jun 2019 02:06:52: 12000000 INFO @ Mon, 03 Jun 2019 02:06:59: 14000000 INFO @ Mon, 03 Jun 2019 02:07:00: 14000000 INFO @ Mon, 03 Jun 2019 02:07:00: 13000000 INFO @ Mon, 03 Jun 2019 02:07:06: 15000000 INFO @ Mon, 03 Jun 2019 02:07:07: 15000000 INFO @ Mon, 03 Jun 2019 02:07:08: 14000000 INFO @ Mon, 03 Jun 2019 02:07:09: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:07:09: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:07:09: #1 total tags in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:07:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:07:10: #1 tags after filtering in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:07:10: #1 finished! INFO @ Mon, 03 Jun 2019 02:07:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:07:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:07:10: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:07:10: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:07:10: #1 total tags in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:07:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:07:10: #1 tags after filtering in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:07:10: #1 finished! INFO @ Mon, 03 Jun 2019 02:07:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:07:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:07:11: #2 number of paired peaks: 36 WARNING @ Mon, 03 Jun 2019 02:07:11: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:07:12: #2 number of paired peaks: 36 WARNING @ Mon, 03 Jun 2019 02:07:12: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:07:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:07:16: 15000000 INFO @ Mon, 03 Jun 2019 02:07:19: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:07:19: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:07:19: #1 total tags in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:07:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:07:19: #1 tags after filtering in treatment: 15444663 INFO @ Mon, 03 Jun 2019 02:07:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:07:19: #1 finished! INFO @ Mon, 03 Jun 2019 02:07:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:07:21: #2 number of paired peaks: 36 WARNING @ Mon, 03 Jun 2019 02:07:21: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:07:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111800/SRX111800.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。