Job ID = 1293711 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,924,150 reads read : 16,924,150 reads written : 16,924,150 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:05:11 16924150 reads; of these: 16924150 (100.00%) were unpaired; of these: 262947 (1.55%) aligned 0 times 13541158 (80.01%) aligned exactly 1 time 3120045 (18.44%) aligned >1 times 98.45% overall alignment rate Time searching: 00:05:12 Overall time: 00:05:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2592584 / 16661203 = 0.1556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:03:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:03:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:03:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:03:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:03:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:03:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:03:38: 1000000 INFO @ Mon, 03 Jun 2019 02:03:38: 1000000 INFO @ Mon, 03 Jun 2019 02:03:38: 1000000 INFO @ Mon, 03 Jun 2019 02:03:46: 2000000 INFO @ Mon, 03 Jun 2019 02:03:46: 2000000 INFO @ Mon, 03 Jun 2019 02:03:46: 2000000 INFO @ Mon, 03 Jun 2019 02:03:54: 3000000 INFO @ Mon, 03 Jun 2019 02:03:54: 3000000 INFO @ Mon, 03 Jun 2019 02:03:54: 3000000 INFO @ Mon, 03 Jun 2019 02:04:01: 4000000 INFO @ Mon, 03 Jun 2019 02:04:02: 4000000 INFO @ Mon, 03 Jun 2019 02:04:02: 4000000 INFO @ Mon, 03 Jun 2019 02:04:09: 5000000 INFO @ Mon, 03 Jun 2019 02:04:09: 5000000 INFO @ Mon, 03 Jun 2019 02:04:10: 5000000 INFO @ Mon, 03 Jun 2019 02:04:17: 6000000 INFO @ Mon, 03 Jun 2019 02:04:17: 6000000 INFO @ Mon, 03 Jun 2019 02:04:17: 6000000 INFO @ Mon, 03 Jun 2019 02:04:25: 7000000 INFO @ Mon, 03 Jun 2019 02:04:25: 7000000 INFO @ Mon, 03 Jun 2019 02:04:25: 7000000 INFO @ Mon, 03 Jun 2019 02:04:33: 8000000 INFO @ Mon, 03 Jun 2019 02:04:33: 8000000 INFO @ Mon, 03 Jun 2019 02:04:34: 8000000 INFO @ Mon, 03 Jun 2019 02:04:40: 9000000 INFO @ Mon, 03 Jun 2019 02:04:41: 9000000 INFO @ Mon, 03 Jun 2019 02:04:42: 9000000 INFO @ Mon, 03 Jun 2019 02:04:48: 10000000 INFO @ Mon, 03 Jun 2019 02:04:48: 10000000 INFO @ Mon, 03 Jun 2019 02:04:50: 10000000 INFO @ Mon, 03 Jun 2019 02:04:55: 11000000 INFO @ Mon, 03 Jun 2019 02:04:56: 11000000 INFO @ Mon, 03 Jun 2019 02:04:58: 11000000 INFO @ Mon, 03 Jun 2019 02:05:03: 12000000 INFO @ Mon, 03 Jun 2019 02:05:04: 12000000 INFO @ Mon, 03 Jun 2019 02:05:07: 12000000 INFO @ Mon, 03 Jun 2019 02:05:11: 13000000 INFO @ Mon, 03 Jun 2019 02:05:11: 13000000 INFO @ Mon, 03 Jun 2019 02:05:15: 13000000 INFO @ Mon, 03 Jun 2019 02:05:18: 14000000 INFO @ Mon, 03 Jun 2019 02:05:19: 14000000 INFO @ Mon, 03 Jun 2019 02:05:19: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:05:19: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:05:19: #1 total tags in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:05:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:05:19: #1 tags after filtering in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:05:19: #1 finished! INFO @ Mon, 03 Jun 2019 02:05:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:05:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:05:19: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:05:19: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:05:19: #1 total tags in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:05:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:05:20: #1 tags after filtering in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:05:20: #1 finished! INFO @ Mon, 03 Jun 2019 02:05:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:05:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:05:21: #2 number of paired peaks: 49 WARNING @ Mon, 03 Jun 2019 02:05:21: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:05:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:05:21: #2 number of paired peaks: 49 WARNING @ Mon, 03 Jun 2019 02:05:21: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:05:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:05:23: 14000000 INFO @ Mon, 03 Jun 2019 02:05:24: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:05:24: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:05:24: #1 total tags in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:05:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:05:24: #1 tags after filtering in treatment: 14068619 INFO @ Mon, 03 Jun 2019 02:05:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:05:24: #1 finished! INFO @ Mon, 03 Jun 2019 02:05:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:05:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:05:25: #2 number of paired peaks: 49 WARNING @ Mon, 03 Jun 2019 02:05:25: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:05:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111790/SRX111790.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。