Job ID = 1293708 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,845,454 reads read : 19,845,454 reads written : 19,845,454 spots read : 22,516,956 reads read : 22,516,956 reads written : 22,516,956 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:53 42362410 reads; of these: 42362410 (100.00%) were unpaired; of these: 6845489 (16.16%) aligned 0 times 28614525 (67.55%) aligned exactly 1 time 6902396 (16.29%) aligned >1 times 83.84% overall alignment rate Time searching: 00:11:53 Overall time: 00:11:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11585900 / 35516921 = 0.3262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:29:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:29:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:29:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:29:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:29:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:29:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:29:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:29:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:29:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:29:31: 1000000 INFO @ Mon, 03 Jun 2019 02:29:32: 1000000 INFO @ Mon, 03 Jun 2019 02:29:32: 1000000 INFO @ Mon, 03 Jun 2019 02:29:37: 2000000 INFO @ Mon, 03 Jun 2019 02:29:39: 2000000 INFO @ Mon, 03 Jun 2019 02:29:40: 2000000 INFO @ Mon, 03 Jun 2019 02:29:44: 3000000 INFO @ Mon, 03 Jun 2019 02:29:46: 3000000 INFO @ Mon, 03 Jun 2019 02:29:48: 3000000 INFO @ Mon, 03 Jun 2019 02:29:50: 4000000 INFO @ Mon, 03 Jun 2019 02:29:53: 4000000 INFO @ Mon, 03 Jun 2019 02:29:56: 4000000 INFO @ Mon, 03 Jun 2019 02:29:57: 5000000 INFO @ Mon, 03 Jun 2019 02:30:00: 5000000 INFO @ Mon, 03 Jun 2019 02:30:03: 6000000 INFO @ Mon, 03 Jun 2019 02:30:04: 5000000 INFO @ Mon, 03 Jun 2019 02:30:07: 6000000 INFO @ Mon, 03 Jun 2019 02:30:10: 7000000 INFO @ Mon, 03 Jun 2019 02:30:12: 6000000 INFO @ Mon, 03 Jun 2019 02:30:14: 7000000 INFO @ Mon, 03 Jun 2019 02:30:16: 8000000 INFO @ Mon, 03 Jun 2019 02:30:20: 7000000 INFO @ Mon, 03 Jun 2019 02:30:21: 8000000 INFO @ Mon, 03 Jun 2019 02:30:23: 9000000 INFO @ Mon, 03 Jun 2019 02:30:28: 9000000 INFO @ Mon, 03 Jun 2019 02:30:28: 8000000 INFO @ Mon, 03 Jun 2019 02:30:29: 10000000 INFO @ Mon, 03 Jun 2019 02:30:36: 10000000 INFO @ Mon, 03 Jun 2019 02:30:36: 11000000 INFO @ Mon, 03 Jun 2019 02:30:36: 9000000 INFO @ Mon, 03 Jun 2019 02:30:42: 12000000 INFO @ Mon, 03 Jun 2019 02:30:43: 11000000 INFO @ Mon, 03 Jun 2019 02:30:44: 10000000 INFO @ Mon, 03 Jun 2019 02:30:49: 13000000 INFO @ Mon, 03 Jun 2019 02:30:50: 12000000 INFO @ Mon, 03 Jun 2019 02:30:52: 11000000 INFO @ Mon, 03 Jun 2019 02:30:55: 14000000 INFO @ Mon, 03 Jun 2019 02:30:57: 13000000 INFO @ Mon, 03 Jun 2019 02:31:00: 12000000 INFO @ Mon, 03 Jun 2019 02:31:02: 15000000 INFO @ Mon, 03 Jun 2019 02:31:04: 14000000 INFO @ Mon, 03 Jun 2019 02:31:08: 13000000 INFO @ Mon, 03 Jun 2019 02:31:08: 16000000 INFO @ Mon, 03 Jun 2019 02:31:11: 15000000 INFO @ Mon, 03 Jun 2019 02:31:15: 17000000 INFO @ Mon, 03 Jun 2019 02:31:16: 14000000 INFO @ Mon, 03 Jun 2019 02:31:19: 16000000 INFO @ Mon, 03 Jun 2019 02:31:21: 18000000 INFO @ Mon, 03 Jun 2019 02:31:24: 15000000 INFO @ Mon, 03 Jun 2019 02:31:26: 17000000 INFO @ Mon, 03 Jun 2019 02:31:28: 19000000 INFO @ Mon, 03 Jun 2019 02:31:32: 16000000 INFO @ Mon, 03 Jun 2019 02:31:33: 18000000 INFO @ Mon, 03 Jun 2019 02:31:34: 20000000 INFO @ Mon, 03 Jun 2019 02:31:39: 17000000 INFO @ Mon, 03 Jun 2019 02:31:41: 21000000 INFO @ Mon, 03 Jun 2019 02:31:41: 19000000 INFO @ Mon, 03 Jun 2019 02:31:47: 22000000 INFO @ Mon, 03 Jun 2019 02:31:47: 18000000 INFO @ Mon, 03 Jun 2019 02:31:48: 20000000 INFO @ Mon, 03 Jun 2019 02:31:54: 23000000 INFO @ Mon, 03 Jun 2019 02:31:55: 19000000 INFO @ Mon, 03 Jun 2019 02:31:56: 21000000 INFO @ Mon, 03 Jun 2019 02:32:00: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:32:00: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:32:00: #1 total tags in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:32:00: #1 tags after filtering in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:32:00: #1 finished! INFO @ Mon, 03 Jun 2019 02:32:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:32:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:32:02: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 02:32:02: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:32:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:32:03: 22000000 INFO @ Mon, 03 Jun 2019 02:32:03: 20000000 INFO @ Mon, 03 Jun 2019 02:32:10: 23000000 INFO @ Mon, 03 Jun 2019 02:32:11: 21000000 INFO @ Mon, 03 Jun 2019 02:32:17: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:32:17: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:32:17: #1 total tags in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:32:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:32:18: #1 tags after filtering in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:32:18: #1 finished! INFO @ Mon, 03 Jun 2019 02:32:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:32:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:32:19: 22000000 INFO @ Mon, 03 Jun 2019 02:32:20: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 02:32:20: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:32:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:32:27: 23000000 INFO @ Mon, 03 Jun 2019 02:32:34: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:32:34: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:32:34: #1 total tags in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:32:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:32:35: #1 tags after filtering in treatment: 23931021 INFO @ Mon, 03 Jun 2019 02:32:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:32:35: #1 finished! INFO @ Mon, 03 Jun 2019 02:32:35: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:32:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:32:37: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 02:32:37: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:32:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111787/SRX111787.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。