Job ID = 1293699 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,820,199 reads read : 24,820,199 reads written : 24,820,199 spots read : 19,301,994 reads read : 19,301,994 reads written : 19,301,994 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:37 44122193 reads; of these: 44122193 (100.00%) were unpaired; of these: 4038015 (9.15%) aligned 0 times 31107733 (70.50%) aligned exactly 1 time 8976445 (20.34%) aligned >1 times 90.85% overall alignment rate Time searching: 00:12:38 Overall time: 00:12:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12420008 / 40084178 = 0.3098 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:14:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:14:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:14:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:14:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:14:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:14:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:14:43: 1000000 INFO @ Mon, 03 Jun 2019 02:14:43: 1000000 INFO @ Mon, 03 Jun 2019 02:14:43: 1000000 INFO @ Mon, 03 Jun 2019 02:14:51: 2000000 INFO @ Mon, 03 Jun 2019 02:14:51: 2000000 INFO @ Mon, 03 Jun 2019 02:14:53: 2000000 INFO @ Mon, 03 Jun 2019 02:15:00: 3000000 INFO @ Mon, 03 Jun 2019 02:15:00: 3000000 INFO @ Mon, 03 Jun 2019 02:15:02: 3000000 INFO @ Mon, 03 Jun 2019 02:15:08: 4000000 INFO @ Mon, 03 Jun 2019 02:15:09: 4000000 INFO @ Mon, 03 Jun 2019 02:15:11: 4000000 INFO @ Mon, 03 Jun 2019 02:15:16: 5000000 INFO @ Mon, 03 Jun 2019 02:15:17: 5000000 INFO @ Mon, 03 Jun 2019 02:15:20: 5000000 INFO @ Mon, 03 Jun 2019 02:15:24: 6000000 INFO @ Mon, 03 Jun 2019 02:15:25: 6000000 INFO @ Mon, 03 Jun 2019 02:15:29: 6000000 INFO @ Mon, 03 Jun 2019 02:15:33: 7000000 INFO @ Mon, 03 Jun 2019 02:15:34: 7000000 INFO @ Mon, 03 Jun 2019 02:15:38: 7000000 INFO @ Mon, 03 Jun 2019 02:15:41: 8000000 INFO @ Mon, 03 Jun 2019 02:15:42: 8000000 INFO @ Mon, 03 Jun 2019 02:15:47: 8000000 INFO @ Mon, 03 Jun 2019 02:15:50: 9000000 INFO @ Mon, 03 Jun 2019 02:15:50: 9000000 INFO @ Mon, 03 Jun 2019 02:15:57: 9000000 INFO @ Mon, 03 Jun 2019 02:15:58: 10000000 INFO @ Mon, 03 Jun 2019 02:15:58: 10000000 INFO @ Mon, 03 Jun 2019 02:16:06: 11000000 INFO @ Mon, 03 Jun 2019 02:16:06: 10000000 INFO @ Mon, 03 Jun 2019 02:16:07: 11000000 INFO @ Mon, 03 Jun 2019 02:16:15: 12000000 INFO @ Mon, 03 Jun 2019 02:16:16: 12000000 INFO @ Mon, 03 Jun 2019 02:16:16: 11000000 INFO @ Mon, 03 Jun 2019 02:16:23: 13000000 INFO @ Mon, 03 Jun 2019 02:16:24: 13000000 INFO @ Mon, 03 Jun 2019 02:16:25: 12000000 INFO @ Mon, 03 Jun 2019 02:16:32: 14000000 INFO @ Mon, 03 Jun 2019 02:16:32: 14000000 INFO @ Mon, 03 Jun 2019 02:16:34: 13000000 INFO @ Mon, 03 Jun 2019 02:16:40: 15000000 INFO @ Mon, 03 Jun 2019 02:16:40: 15000000 INFO @ Mon, 03 Jun 2019 02:16:44: 14000000 INFO @ Mon, 03 Jun 2019 02:16:48: 16000000 INFO @ Mon, 03 Jun 2019 02:16:49: 16000000 INFO @ Mon, 03 Jun 2019 02:16:52: 15000000 INFO @ Mon, 03 Jun 2019 02:16:56: 17000000 INFO @ Mon, 03 Jun 2019 02:16:57: 17000000 INFO @ Mon, 03 Jun 2019 02:17:01: 16000000 INFO @ Mon, 03 Jun 2019 02:17:04: 18000000 INFO @ Mon, 03 Jun 2019 02:17:05: 18000000 INFO @ Mon, 03 Jun 2019 02:17:10: 17000000 INFO @ Mon, 03 Jun 2019 02:17:12: 19000000 INFO @ Mon, 03 Jun 2019 02:17:13: 19000000 INFO @ Mon, 03 Jun 2019 02:17:19: 20000000 INFO @ Mon, 03 Jun 2019 02:17:20: 18000000 INFO @ Mon, 03 Jun 2019 02:17:21: 20000000 INFO @ Mon, 03 Jun 2019 02:17:27: 21000000 INFO @ Mon, 03 Jun 2019 02:17:29: 19000000 INFO @ Mon, 03 Jun 2019 02:17:30: 21000000 INFO @ Mon, 03 Jun 2019 02:17:36: 22000000 INFO @ Mon, 03 Jun 2019 02:17:38: 22000000 INFO @ Mon, 03 Jun 2019 02:17:38: 20000000 INFO @ Mon, 03 Jun 2019 02:17:45: 23000000 INFO @ Mon, 03 Jun 2019 02:17:46: 23000000 INFO @ Mon, 03 Jun 2019 02:17:47: 21000000 INFO @ Mon, 03 Jun 2019 02:17:53: 24000000 INFO @ Mon, 03 Jun 2019 02:17:54: 24000000 INFO @ Mon, 03 Jun 2019 02:17:56: 22000000 INFO @ Mon, 03 Jun 2019 02:18:01: 25000000 INFO @ Mon, 03 Jun 2019 02:18:04: 25000000 INFO @ Mon, 03 Jun 2019 02:18:05: 23000000 INFO @ Mon, 03 Jun 2019 02:18:09: 26000000 INFO @ Mon, 03 Jun 2019 02:18:12: 26000000 INFO @ Mon, 03 Jun 2019 02:18:14: 24000000 INFO @ Mon, 03 Jun 2019 02:18:17: 27000000 INFO @ Mon, 03 Jun 2019 02:18:20: 27000000 INFO @ Mon, 03 Jun 2019 02:18:23: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:18:23: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:18:23: #1 total tags in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:18:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:18:23: 25000000 INFO @ Mon, 03 Jun 2019 02:18:23: #1 tags after filtering in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:18:23: #1 finished! INFO @ Mon, 03 Jun 2019 02:18:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:18:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:18:25: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:18:25: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:18:25: #1 total tags in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:18:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:18:26: #2 number of paired peaks: 4 WARNING @ Mon, 03 Jun 2019 02:18:26: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:18:26: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 02:18:26: #1 tags after filtering in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:18:26: #1 finished! INFO @ Mon, 03 Jun 2019 02:18:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:18:26: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:18:28: #2 number of paired peaks: 4 WARNING @ Mon, 03 Jun 2019 02:18:28: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:18:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:18:32: 26000000 INFO @ Mon, 03 Jun 2019 02:18:40: 27000000 INFO @ Mon, 03 Jun 2019 02:18:47: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:18:47: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:18:47: #1 total tags in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:18:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:18:47: #1 tags after filtering in treatment: 27664170 INFO @ Mon, 03 Jun 2019 02:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:18:47: #1 finished! INFO @ Mon, 03 Jun 2019 02:18:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:18:49: #2 number of paired peaks: 4 WARNING @ Mon, 03 Jun 2019 02:18:49: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:18:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111781/SRX111781.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。