Job ID = 6527554 SRX = SRX110793 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:19:11 prefetch.2.10.7: 1) Downloading 'SRR388375'... 2020-06-29T12:19:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:20:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:20:48 prefetch.2.10.7: 'SRR388375' is valid 2020-06-29T12:20:48 prefetch.2.10.7: 1) 'SRR388375' was downloaded successfully Read 23332337 spots for SRR388375/SRR388375.sra Written 23332337 spots for SRR388375/SRR388375.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:08 23332337 reads; of these: 23332337 (100.00%) were unpaired; of these: 652483 (2.80%) aligned 0 times 12104448 (51.88%) aligned exactly 1 time 10575406 (45.33%) aligned >1 times 97.20% overall alignment rate Time searching: 00:07:09 Overall time: 00:07:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2024303 / 22679854 = 0.0893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:36:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:36:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:36:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:36:44: 1000000 INFO @ Mon, 29 Jun 2020 21:36:49: 2000000 INFO @ Mon, 29 Jun 2020 21:36:54: 3000000 INFO @ Mon, 29 Jun 2020 21:37:00: 4000000 INFO @ Mon, 29 Jun 2020 21:37:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:37:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:37:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:37:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:37:10: 6000000 INFO @ Mon, 29 Jun 2020 21:37:14: 1000000 INFO @ Mon, 29 Jun 2020 21:37:16: 7000000 INFO @ Mon, 29 Jun 2020 21:37:19: 2000000 INFO @ Mon, 29 Jun 2020 21:37:21: 8000000 INFO @ Mon, 29 Jun 2020 21:37:24: 3000000 INFO @ Mon, 29 Jun 2020 21:37:26: 9000000 INFO @ Mon, 29 Jun 2020 21:37:29: 4000000 INFO @ Mon, 29 Jun 2020 21:37:31: 10000000 INFO @ Mon, 29 Jun 2020 21:37:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:37:36: 11000000 INFO @ Mon, 29 Jun 2020 21:37:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:37:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:37:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:37:40: 6000000 INFO @ Mon, 29 Jun 2020 21:37:41: 12000000 INFO @ Mon, 29 Jun 2020 21:37:44: 1000000 INFO @ Mon, 29 Jun 2020 21:37:45: 7000000 INFO @ Mon, 29 Jun 2020 21:37:46: 13000000 INFO @ Mon, 29 Jun 2020 21:37:50: 2000000 INFO @ Mon, 29 Jun 2020 21:37:50: 8000000 INFO @ Mon, 29 Jun 2020 21:37:52: 14000000 INFO @ Mon, 29 Jun 2020 21:37:54: 3000000 INFO @ Mon, 29 Jun 2020 21:37:55: 9000000 INFO @ Mon, 29 Jun 2020 21:37:57: 15000000 INFO @ Mon, 29 Jun 2020 21:37:59: 4000000 INFO @ Mon, 29 Jun 2020 21:38:00: 10000000 INFO @ Mon, 29 Jun 2020 21:38:02: 16000000 INFO @ Mon, 29 Jun 2020 21:38:04: 5000000 INFO @ Mon, 29 Jun 2020 21:38:05: 11000000 INFO @ Mon, 29 Jun 2020 21:38:07: 17000000 INFO @ Mon, 29 Jun 2020 21:38:09: 6000000 INFO @ Mon, 29 Jun 2020 21:38:10: 12000000 INFO @ Mon, 29 Jun 2020 21:38:12: 18000000 INFO @ Mon, 29 Jun 2020 21:38:14: 7000000 INFO @ Mon, 29 Jun 2020 21:38:15: 13000000 INFO @ Mon, 29 Jun 2020 21:38:17: 19000000 INFO @ Mon, 29 Jun 2020 21:38:19: 8000000 INFO @ Mon, 29 Jun 2020 21:38:20: 14000000 INFO @ Mon, 29 Jun 2020 21:38:22: 20000000 INFO @ Mon, 29 Jun 2020 21:38:24: 9000000 INFO @ Mon, 29 Jun 2020 21:38:25: 15000000 INFO @ Mon, 29 Jun 2020 21:38:26: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 21:38:26: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 21:38:26: #1 total tags in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:38:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:38:26: #1 tags after filtering in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:38:26: #1 finished! INFO @ Mon, 29 Jun 2020 21:38:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:38:27: #2 number of paired peaks: 30 WARNING @ Mon, 29 Jun 2020 21:38:27: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:38:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:38:29: 10000000 INFO @ Mon, 29 Jun 2020 21:38:30: 16000000 INFO @ Mon, 29 Jun 2020 21:38:33: 11000000 INFO @ Mon, 29 Jun 2020 21:38:35: 17000000 INFO @ Mon, 29 Jun 2020 21:38:38: 12000000 INFO @ Mon, 29 Jun 2020 21:38:39: 18000000 INFO @ Mon, 29 Jun 2020 21:38:43: 13000000 INFO @ Mon, 29 Jun 2020 21:38:44: 19000000 INFO @ Mon, 29 Jun 2020 21:38:48: 14000000 INFO @ Mon, 29 Jun 2020 21:38:49: 20000000 INFO @ Mon, 29 Jun 2020 21:38:52: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 21:38:52: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 21:38:52: #1 total tags in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:38:52: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:38:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:38:52: #1 tags after filtering in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:38:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:38:52: #1 finished! INFO @ Mon, 29 Jun 2020 21:38:52: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:38:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:38:53: 15000000 INFO @ Mon, 29 Jun 2020 21:38:54: #2 number of paired peaks: 30 WARNING @ Mon, 29 Jun 2020 21:38:54: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:38:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:38:57: 16000000 INFO @ Mon, 29 Jun 2020 21:39:02: 17000000 INFO @ Mon, 29 Jun 2020 21:39:07: 18000000 INFO @ Mon, 29 Jun 2020 21:39:11: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 21:39:16: 20000000 INFO @ Mon, 29 Jun 2020 21:39:19: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 21:39:19: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 21:39:19: #1 total tags in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:39:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:39:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:39:20: #1 tags after filtering in treatment: 20655551 INFO @ Mon, 29 Jun 2020 21:39:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:39:20: #1 finished! INFO @ Mon, 29 Jun 2020 21:39:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:39:21: #2 number of paired peaks: 30 WARNING @ Mon, 29 Jun 2020 21:39:21: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:39:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110793/SRX110793.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。