Job ID = 6527552 SRX = SRX110791 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:49:57 prefetch.2.10.7: 1) Downloading 'SRR388373'... 2020-06-29T12:49:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:54:43 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:54:43 prefetch.2.10.7: 1) 'SRR388373' was downloaded successfully Read 41972385 spots for SRR388373/SRR388373.sra Written 41972385 spots for SRR388373/SRR388373.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:35 41972385 reads; of these: 41972385 (100.00%) were unpaired; of these: 2694454 (6.42%) aligned 0 times 33218278 (79.14%) aligned exactly 1 time 6059653 (14.44%) aligned >1 times 93.58% overall alignment rate Time searching: 00:09:36 Overall time: 00:09:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8126561 / 39277931 = 0.2069 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:27:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:27:09: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:27:09: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:27:16: 1000000 INFO @ Mon, 29 Jun 2020 22:27:23: 2000000 INFO @ Mon, 29 Jun 2020 22:27:30: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:27:37: 4000000 INFO @ Mon, 29 Jun 2020 22:27:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:27:39: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:27:39: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:27:44: 5000000 INFO @ Mon, 29 Jun 2020 22:27:46: 1000000 INFO @ Mon, 29 Jun 2020 22:27:51: 6000000 INFO @ Mon, 29 Jun 2020 22:27:53: 2000000 INFO @ Mon, 29 Jun 2020 22:27:58: 7000000 INFO @ Mon, 29 Jun 2020 22:28:00: 3000000 INFO @ Mon, 29 Jun 2020 22:28:05: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:28:07: 4000000 INFO @ Mon, 29 Jun 2020 22:28:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:28:09: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:28:09: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:28:12: 9000000 INFO @ Mon, 29 Jun 2020 22:28:14: 5000000 INFO @ Mon, 29 Jun 2020 22:28:16: 1000000 INFO @ Mon, 29 Jun 2020 22:28:19: 10000000 INFO @ Mon, 29 Jun 2020 22:28:21: 6000000 INFO @ Mon, 29 Jun 2020 22:28:23: 2000000 INFO @ Mon, 29 Jun 2020 22:28:26: 11000000 INFO @ Mon, 29 Jun 2020 22:28:28: 7000000 INFO @ Mon, 29 Jun 2020 22:28:30: 3000000 INFO @ Mon, 29 Jun 2020 22:28:33: 12000000 INFO @ Mon, 29 Jun 2020 22:28:35: 8000000 INFO @ Mon, 29 Jun 2020 22:28:37: 4000000 INFO @ Mon, 29 Jun 2020 22:28:40: 13000000 INFO @ Mon, 29 Jun 2020 22:28:42: 9000000 INFO @ Mon, 29 Jun 2020 22:28:45: 5000000 INFO @ Mon, 29 Jun 2020 22:28:47: 14000000 INFO @ Mon, 29 Jun 2020 22:28:49: 10000000 INFO @ Mon, 29 Jun 2020 22:28:52: 6000000 INFO @ Mon, 29 Jun 2020 22:28:54: 15000000 INFO @ Mon, 29 Jun 2020 22:28:56: 11000000 INFO @ Mon, 29 Jun 2020 22:28:59: 7000000 INFO @ Mon, 29 Jun 2020 22:29:01: 16000000 INFO @ Mon, 29 Jun 2020 22:29:03: 12000000 INFO @ Mon, 29 Jun 2020 22:29:06: 8000000 INFO @ Mon, 29 Jun 2020 22:29:07: 17000000 INFO @ Mon, 29 Jun 2020 22:29:10: 13000000 INFO @ Mon, 29 Jun 2020 22:29:13: 9000000 INFO @ Mon, 29 Jun 2020 22:29:14: 18000000 INFO @ Mon, 29 Jun 2020 22:29:17: 14000000 INFO @ Mon, 29 Jun 2020 22:29:20: 10000000 INFO @ Mon, 29 Jun 2020 22:29:21: 19000000 INFO @ Mon, 29 Jun 2020 22:29:24: 15000000 INFO @ Mon, 29 Jun 2020 22:29:27: 11000000 INFO @ Mon, 29 Jun 2020 22:29:28: 20000000 INFO @ Mon, 29 Jun 2020 22:29:31: 16000000 INFO @ Mon, 29 Jun 2020 22:29:34: 12000000 INFO @ Mon, 29 Jun 2020 22:29:35: 21000000 INFO @ Mon, 29 Jun 2020 22:29:38: 17000000 INFO @ Mon, 29 Jun 2020 22:29:41: 13000000 INFO @ Mon, 29 Jun 2020 22:29:42: 22000000 INFO @ Mon, 29 Jun 2020 22:29:45: 18000000 INFO @ Mon, 29 Jun 2020 22:29:48: 14000000 INFO @ Mon, 29 Jun 2020 22:29:49: 23000000 INFO @ Mon, 29 Jun 2020 22:29:52: 19000000 INFO @ Mon, 29 Jun 2020 22:29:55: 15000000 INFO @ Mon, 29 Jun 2020 22:29:56: 24000000 INFO @ Mon, 29 Jun 2020 22:29:58: 20000000 INFO @ Mon, 29 Jun 2020 22:30:02: 16000000 INFO @ Mon, 29 Jun 2020 22:30:03: 25000000 INFO @ Mon, 29 Jun 2020 22:30:05: 21000000 INFO @ Mon, 29 Jun 2020 22:30:09: 17000000 INFO @ Mon, 29 Jun 2020 22:30:10: 26000000 INFO @ Mon, 29 Jun 2020 22:30:12: 22000000 INFO @ Mon, 29 Jun 2020 22:30:16: 18000000 INFO @ Mon, 29 Jun 2020 22:30:17: 27000000 INFO @ Mon, 29 Jun 2020 22:30:19: 23000000 INFO @ Mon, 29 Jun 2020 22:30:23: 19000000 INFO @ Mon, 29 Jun 2020 22:30:24: 28000000 INFO @ Mon, 29 Jun 2020 22:30:26: 24000000 INFO @ Mon, 29 Jun 2020 22:30:29: 20000000 INFO @ Mon, 29 Jun 2020 22:30:31: 29000000 INFO @ Mon, 29 Jun 2020 22:30:33: 25000000 INFO @ Mon, 29 Jun 2020 22:30:36: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:30:38: 30000000 INFO @ Mon, 29 Jun 2020 22:30:40: 26000000 INFO @ Mon, 29 Jun 2020 22:30:43: 22000000 INFO @ Mon, 29 Jun 2020 22:30:45: 31000000 INFO @ Mon, 29 Jun 2020 22:30:46: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:30:46: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:30:46: #1 total tags in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:30:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:30:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:30:47: #1 tags after filtering in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:30:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:30:47: #1 finished! INFO @ Mon, 29 Jun 2020 22:30:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:30:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:30:47: 27000000 INFO @ Mon, 29 Jun 2020 22:30:49: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 22:30:49: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:30:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:30:50: 23000000 INFO @ Mon, 29 Jun 2020 22:30:54: 28000000 INFO @ Mon, 29 Jun 2020 22:30:57: 24000000 INFO @ Mon, 29 Jun 2020 22:31:01: 29000000 INFO @ Mon, 29 Jun 2020 22:31:04: 25000000 INFO @ Mon, 29 Jun 2020 22:31:08: 30000000 INFO @ Mon, 29 Jun 2020 22:31:11: 26000000 INFO @ Mon, 29 Jun 2020 22:31:15: 31000000 INFO @ Mon, 29 Jun 2020 22:31:16: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:31:16: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:31:16: #1 total tags in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:31:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:31:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:31:17: #1 tags after filtering in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:31:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:31:17: #1 finished! INFO @ Mon, 29 Jun 2020 22:31:17: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:31:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:31:18: 27000000 INFO @ Mon, 29 Jun 2020 22:31:19: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 22:31:19: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:31:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:31:25: 28000000 INFO @ Mon, 29 Jun 2020 22:31:32: 29000000 INFO @ Mon, 29 Jun 2020 22:31:38: 30000000 INFO @ Mon, 29 Jun 2020 22:31:45: 31000000 INFO @ Mon, 29 Jun 2020 22:31:47: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:31:47: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:31:47: #1 total tags in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:31:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:31:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:31:47: #1 tags after filtering in treatment: 31151370 INFO @ Mon, 29 Jun 2020 22:31:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:31:47: #1 finished! INFO @ Mon, 29 Jun 2020 22:31:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:31:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:31:49: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 22:31:49: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:31:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110791/SRX110791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。