Job ID = 9158202 sra ファイルのダウンロード中... Completed: 674320K bytes transferred in 7 seconds (703843K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21914988 spots for /home/okishinya/chipatlas/results/dm3/SRX110511/SRR387833.sra Written 21914988 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:14 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2148662 (9.80%) aligned 0 times 17615064 (80.38%) aligned exactly 1 time 2151262 (9.82%) aligned >1 times 90.20% overall alignment rate Time searching: 00:06:14 Overall time: 00:06:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3542945 / 19766326 = 0.1792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:50:04: # Command line: callpeak -t SRX110511.bam -f BAM -g dm -n SRX110511.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX110511.10 # format = BAM # ChIP-seq file = ['SRX110511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:50:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:50:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:50:04: # Command line: callpeak -t SRX110511.bam -f BAM -g dm -n SRX110511.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX110511.20 # format = BAM # ChIP-seq file = ['SRX110511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:50:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:50:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:50:04: # Command line: callpeak -t SRX110511.bam -f BAM -g dm -n SRX110511.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX110511.05 # format = BAM # ChIP-seq file = ['SRX110511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:50:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:50:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:50:12: 1000000 INFO @ Tue, 27 Jun 2017 15:50:12: 1000000 INFO @ Tue, 27 Jun 2017 15:50:12: 1000000 INFO @ Tue, 27 Jun 2017 15:50:20: 2000000 INFO @ Tue, 27 Jun 2017 15:50:20: 2000000 INFO @ Tue, 27 Jun 2017 15:50:20: 2000000 INFO @ Tue, 27 Jun 2017 15:50:28: 3000000 INFO @ Tue, 27 Jun 2017 15:50:28: 3000000 INFO @ Tue, 27 Jun 2017 15:50:29: 3000000 INFO @ Tue, 27 Jun 2017 15:50:36: 4000000 INFO @ Tue, 27 Jun 2017 15:50:36: 4000000 INFO @ Tue, 27 Jun 2017 15:50:37: 4000000 INFO @ Tue, 27 Jun 2017 15:50:44: 5000000 INFO @ Tue, 27 Jun 2017 15:50:44: 5000000 INFO @ Tue, 27 Jun 2017 15:50:45: 5000000 INFO @ Tue, 27 Jun 2017 15:50:52: 6000000 INFO @ Tue, 27 Jun 2017 15:50:53: 6000000 INFO @ Tue, 27 Jun 2017 15:50:54: 6000000 INFO @ Tue, 27 Jun 2017 15:51:00: 7000000 INFO @ Tue, 27 Jun 2017 15:51:02: 7000000 INFO @ Tue, 27 Jun 2017 15:51:03: 7000000 INFO @ Tue, 27 Jun 2017 15:51:07: 8000000 INFO @ Tue, 27 Jun 2017 15:51:10: 8000000 INFO @ Tue, 27 Jun 2017 15:51:12: 8000000 INFO @ Tue, 27 Jun 2017 15:51:15: 9000000 INFO @ Tue, 27 Jun 2017 15:51:18: 9000000 INFO @ Tue, 27 Jun 2017 15:51:22: 9000000 INFO @ Tue, 27 Jun 2017 15:51:23: 10000000 INFO @ Tue, 27 Jun 2017 15:51:27: 10000000 INFO @ Tue, 27 Jun 2017 15:51:31: 11000000 INFO @ Tue, 27 Jun 2017 15:51:31: 10000000 INFO @ Tue, 27 Jun 2017 15:51:35: 11000000 INFO @ Tue, 27 Jun 2017 15:51:39: 12000000 INFO @ Tue, 27 Jun 2017 15:51:41: 11000000 INFO @ Tue, 27 Jun 2017 15:51:43: 12000000 INFO @ Tue, 27 Jun 2017 15:51:47: 13000000 INFO @ Tue, 27 Jun 2017 15:51:50: 12000000 INFO @ Tue, 27 Jun 2017 15:51:52: 13000000 INFO @ Tue, 27 Jun 2017 15:51:55: 14000000 INFO @ Tue, 27 Jun 2017 15:52:00: 13000000 INFO @ Tue, 27 Jun 2017 15:52:00: 14000000 INFO @ Tue, 27 Jun 2017 15:52:03: 15000000 INFO @ Tue, 27 Jun 2017 15:52:08: 15000000 INFO @ Tue, 27 Jun 2017 15:52:09: 14000000 INFO @ Tue, 27 Jun 2017 15:52:11: 16000000 INFO @ Tue, 27 Jun 2017 15:52:12: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:52:12: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:52:12: #1 total tags in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:52:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:52:13: #1 tags after filtering in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:52:13: #1 finished! INFO @ Tue, 27 Jun 2017 15:52:13: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:52:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:52:14: #2 number of paired peaks: 17 WARNING @ Tue, 27 Jun 2017 15:52:14: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:52:14: Process for pairing-model is terminated! cat: SRX110511.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX110511.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:52:17: 16000000 INFO @ Tue, 27 Jun 2017 15:52:18: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:52:18: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:52:18: #1 total tags in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:18: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:52:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:52:19: #1 tags after filtering in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:52:19: #1 finished! INFO @ Tue, 27 Jun 2017 15:52:19: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:52:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:52:19: 15000000 INFO @ Tue, 27 Jun 2017 15:52:20: #2 number of paired peaks: 17 WARNING @ Tue, 27 Jun 2017 15:52:20: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:52:20: Process for pairing-model is terminated! cat: SRX110511.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX110511.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:52:28: 16000000 INFO @ Tue, 27 Jun 2017 15:52:30: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:52:30: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:52:30: #1 total tags in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:52:30: #1 tags after filtering in treatment: 16223381 INFO @ Tue, 27 Jun 2017 15:52:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:52:30: #1 finished! INFO @ Tue, 27 Jun 2017 15:52:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:52:31: #2 number of paired peaks: 17 WARNING @ Tue, 27 Jun 2017 15:52:31: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:52:31: Process for pairing-model is terminated! cat: SRX110511.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX110511.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110511.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。