Job ID = 6527548 SRX = SRX1084166 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:01:28 prefetch.2.10.7: 1) Downloading 'SRR2088912'... 2020-06-29T13:01:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:08:50 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:08:50 prefetch.2.10.7: 1) 'SRR2088912' was downloaded successfully Read 36793392 spots for SRR2088912/SRR2088912.sra Written 36793392 spots for SRR2088912/SRR2088912.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:17 36793392 reads; of these: 36793392 (100.00%) were unpaired; of these: 3490572 (9.49%) aligned 0 times 25693029 (69.83%) aligned exactly 1 time 7609791 (20.68%) aligned >1 times 90.51% overall alignment rate Time searching: 00:21:17 Overall time: 00:21:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15046628 / 33302820 = 0.4518 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:08: 1000000 INFO @ Mon, 29 Jun 2020 23:02:17: 2000000 INFO @ Mon, 29 Jun 2020 23:02:25: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:35: 4000000 INFO @ Mon, 29 Jun 2020 23:02:40: 1000000 INFO @ Mon, 29 Jun 2020 23:02:45: 5000000 INFO @ Mon, 29 Jun 2020 23:02:50: 2000000 INFO @ Mon, 29 Jun 2020 23:02:55: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:03:01: 3000000 INFO @ Mon, 29 Jun 2020 23:03:05: 7000000 INFO @ Mon, 29 Jun 2020 23:03:10: 1000000 INFO @ Mon, 29 Jun 2020 23:03:12: 4000000 INFO @ Mon, 29 Jun 2020 23:03:14: 8000000 INFO @ Mon, 29 Jun 2020 23:03:21: 2000000 INFO @ Mon, 29 Jun 2020 23:03:22: 5000000 INFO @ Mon, 29 Jun 2020 23:03:24: 9000000 INFO @ Mon, 29 Jun 2020 23:03:32: 3000000 INFO @ Mon, 29 Jun 2020 23:03:33: 6000000 INFO @ Mon, 29 Jun 2020 23:03:34: 10000000 INFO @ Mon, 29 Jun 2020 23:03:43: 4000000 INFO @ Mon, 29 Jun 2020 23:03:43: 7000000 INFO @ Mon, 29 Jun 2020 23:03:44: 11000000 INFO @ Mon, 29 Jun 2020 23:03:53: 12000000 INFO @ Mon, 29 Jun 2020 23:03:54: 8000000 INFO @ Mon, 29 Jun 2020 23:03:54: 5000000 INFO @ Mon, 29 Jun 2020 23:04:03: 13000000 INFO @ Mon, 29 Jun 2020 23:04:04: 9000000 INFO @ Mon, 29 Jun 2020 23:04:05: 6000000 INFO @ Mon, 29 Jun 2020 23:04:12: 14000000 INFO @ Mon, 29 Jun 2020 23:04:14: 10000000 INFO @ Mon, 29 Jun 2020 23:04:15: 7000000 INFO @ Mon, 29 Jun 2020 23:04:22: 15000000 INFO @ Mon, 29 Jun 2020 23:04:25: 11000000 INFO @ Mon, 29 Jun 2020 23:04:26: 8000000 INFO @ Mon, 29 Jun 2020 23:04:31: 16000000 INFO @ Mon, 29 Jun 2020 23:04:35: 12000000 INFO @ Mon, 29 Jun 2020 23:04:36: 9000000 INFO @ Mon, 29 Jun 2020 23:04:41: 17000000 INFO @ Mon, 29 Jun 2020 23:04:45: 10000000 INFO @ Mon, 29 Jun 2020 23:04:45: 13000000 INFO @ Mon, 29 Jun 2020 23:04:50: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:04:53: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:04:53: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:04:53: #1 total tags in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:04:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:53: #1 tags after filtering in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:04:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:53: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:04:54: #2 number of paired peaks: 98 WARNING @ Mon, 29 Jun 2020 23:04:54: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:04:55: 11000000 INFO @ Mon, 29 Jun 2020 23:04:56: 14000000 INFO @ Mon, 29 Jun 2020 23:05:04: 12000000 INFO @ Mon, 29 Jun 2020 23:05:06: 15000000 INFO @ Mon, 29 Jun 2020 23:05:13: 13000000 INFO @ Mon, 29 Jun 2020 23:05:16: 16000000 INFO @ Mon, 29 Jun 2020 23:05:22: 14000000 INFO @ Mon, 29 Jun 2020 23:05:26: 17000000 INFO @ Mon, 29 Jun 2020 23:05:31: 15000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:05:36: 18000000 INFO @ Mon, 29 Jun 2020 23:05:39: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:05:39: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:05:39: #1 total tags in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:05:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:39: #1 tags after filtering in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:39: 16000000 INFO @ Mon, 29 Jun 2020 23:05:40: #2 number of paired peaks: 98 WARNING @ Mon, 29 Jun 2020 23:05:40: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:05:47: 17000000 INFO @ Mon, 29 Jun 2020 23:05:55: 18000000 INFO @ Mon, 29 Jun 2020 23:05:57: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:05:57: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:05:57: #1 total tags in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:05:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:58: #1 tags after filtering in treatment: 18256192 INFO @ Mon, 29 Jun 2020 23:05:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:59: #2 number of paired peaks: 98 WARNING @ Mon, 29 Jun 2020 23:05:59: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084166/SRX1084166.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling