Job ID = 6527544 SRX = SRX1084162 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:00:57 prefetch.2.10.7: 1) Downloading 'SRR2088908'... 2020-06-29T13:00:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:03:43 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:03:43 prefetch.2.10.7: 1) 'SRR2088908' was downloaded successfully Read 21759793 spots for SRR2088908/SRR2088908.sra Written 21759793 spots for SRR2088908/SRR2088908.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:58 21759793 reads; of these: 21759793 (100.00%) were unpaired; of these: 4209115 (19.34%) aligned 0 times 13927989 (64.01%) aligned exactly 1 time 3622689 (16.65%) aligned >1 times 80.66% overall alignment rate Time searching: 00:05:58 Overall time: 00:05:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1336285 / 17550678 = 0.0761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:21:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:21:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:21:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:21:38: 1000000 INFO @ Mon, 29 Jun 2020 22:21:44: 2000000 INFO @ Mon, 29 Jun 2020 22:21:51: 3000000 INFO @ Mon, 29 Jun 2020 22:21:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:22:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:22:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:22:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:22:04: 5000000 INFO @ Mon, 29 Jun 2020 22:22:09: 1000000 INFO @ Mon, 29 Jun 2020 22:22:12: 6000000 INFO @ Mon, 29 Jun 2020 22:22:19: 2000000 INFO @ Mon, 29 Jun 2020 22:22:21: 7000000 INFO @ Mon, 29 Jun 2020 22:22:28: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:22:29: 8000000 INFO @ Mon, 29 Jun 2020 22:22:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:22:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:22:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:22:37: 9000000 INFO @ Mon, 29 Jun 2020 22:22:37: 4000000 INFO @ Mon, 29 Jun 2020 22:22:40: 1000000 INFO @ Mon, 29 Jun 2020 22:22:46: 10000000 INFO @ Mon, 29 Jun 2020 22:22:46: 5000000 INFO @ Mon, 29 Jun 2020 22:22:49: 2000000 INFO @ Mon, 29 Jun 2020 22:22:54: 11000000 INFO @ Mon, 29 Jun 2020 22:22:55: 6000000 INFO @ Mon, 29 Jun 2020 22:22:58: 3000000 INFO @ Mon, 29 Jun 2020 22:23:03: 12000000 INFO @ Mon, 29 Jun 2020 22:23:05: 7000000 INFO @ Mon, 29 Jun 2020 22:23:07: 4000000 INFO @ Mon, 29 Jun 2020 22:23:11: 13000000 INFO @ Mon, 29 Jun 2020 22:23:14: 8000000 INFO @ Mon, 29 Jun 2020 22:23:16: 5000000 INFO @ Mon, 29 Jun 2020 22:23:20: 14000000 INFO @ Mon, 29 Jun 2020 22:23:23: 9000000 INFO @ Mon, 29 Jun 2020 22:23:24: 6000000 INFO @ Mon, 29 Jun 2020 22:23:28: 15000000 INFO @ Mon, 29 Jun 2020 22:23:32: 10000000 INFO @ Mon, 29 Jun 2020 22:23:33: 7000000 INFO @ Mon, 29 Jun 2020 22:23:36: 16000000 INFO @ Mon, 29 Jun 2020 22:23:38: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:23:38: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:23:38: #1 total tags in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:23:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:23:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:23:38: #1 tags after filtering in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:23:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:23:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:23:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:23:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:23:39: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 22:23:39: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:23:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:23:40: 11000000 INFO @ Mon, 29 Jun 2020 22:23:42: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:23:49: 12000000 INFO @ Mon, 29 Jun 2020 22:23:50: 9000000 INFO @ Mon, 29 Jun 2020 22:23:58: 13000000 INFO @ Mon, 29 Jun 2020 22:23:59: 10000000 INFO @ Mon, 29 Jun 2020 22:24:07: 14000000 INFO @ Mon, 29 Jun 2020 22:24:07: 11000000 INFO @ Mon, 29 Jun 2020 22:24:16: 15000000 INFO @ Mon, 29 Jun 2020 22:24:16: 12000000 INFO @ Mon, 29 Jun 2020 22:24:25: 16000000 INFO @ Mon, 29 Jun 2020 22:24:25: 13000000 INFO @ Mon, 29 Jun 2020 22:24:26: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:24:26: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:24:26: #1 total tags in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:24:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:24:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:24:27: #1 tags after filtering in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:24:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:24:27: #1 finished! INFO @ Mon, 29 Jun 2020 22:24:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:24:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:24:28: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 22:24:28: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:24:28: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:24:33: 14000000 INFO @ Mon, 29 Jun 2020 22:24:41: 15000000 INFO @ Mon, 29 Jun 2020 22:24:49: 16000000 INFO @ Mon, 29 Jun 2020 22:24:50: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:24:50: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:24:50: #1 total tags in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:24:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:24:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:24:50: #1 tags after filtering in treatment: 16214393 INFO @ Mon, 29 Jun 2020 22:24:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:24:50: #1 finished! INFO @ Mon, 29 Jun 2020 22:24:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:24:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:24:52: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 22:24:52: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:24:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084162/SRX1084162.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling