Job ID = 6527532 SRX = SRX104975 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:34:41 prefetch.2.10.7: 1) Downloading 'SRR363419'... 2020-06-29T12:34:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:36:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:36:15 prefetch.2.10.7: 'SRR363419' is valid 2020-06-29T12:36:15 prefetch.2.10.7: 1) 'SRR363419' was downloaded successfully Read 18883034 spots for SRR363419/SRR363419.sra Written 18883034 spots for SRR363419/SRR363419.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:35 18883034 reads; of these: 18883034 (100.00%) were unpaired; of these: 672227 (3.56%) aligned 0 times 15013541 (79.51%) aligned exactly 1 time 3197266 (16.93%) aligned >1 times 96.44% overall alignment rate Time searching: 00:04:35 Overall time: 00:04:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2045972 / 18210807 = 0.1123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:50:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:50:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:50:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:50:18: 1000000 INFO @ Mon, 29 Jun 2020 21:50:23: 2000000 INFO @ Mon, 29 Jun 2020 21:50:28: 3000000 INFO @ Mon, 29 Jun 2020 21:50:32: 4000000 INFO @ Mon, 29 Jun 2020 21:50:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:50:42: 6000000 INFO @ Mon, 29 Jun 2020 21:50:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:50:43: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:50:43: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:50:46: 7000000 INFO @ Mon, 29 Jun 2020 21:50:49: 1000000 INFO @ Mon, 29 Jun 2020 21:50:51: 8000000 INFO @ Mon, 29 Jun 2020 21:50:54: 2000000 INFO @ Mon, 29 Jun 2020 21:50:56: 9000000 INFO @ Mon, 29 Jun 2020 21:50:59: 3000000 INFO @ Mon, 29 Jun 2020 21:51:01: 10000000 INFO @ Mon, 29 Jun 2020 21:51:04: 4000000 INFO @ Mon, 29 Jun 2020 21:51:06: 11000000 INFO @ Mon, 29 Jun 2020 21:51:09: 5000000 INFO @ Mon, 29 Jun 2020 21:51:11: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:51:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:51:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:51:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:51:14: 6000000 INFO @ Mon, 29 Jun 2020 21:51:16: 13000000 INFO @ Mon, 29 Jun 2020 21:51:19: 1000000 INFO @ Mon, 29 Jun 2020 21:51:19: 7000000 INFO @ Mon, 29 Jun 2020 21:51:21: 14000000 INFO @ Mon, 29 Jun 2020 21:51:24: 8000000 INFO @ Mon, 29 Jun 2020 21:51:24: 2000000 INFO @ Mon, 29 Jun 2020 21:51:27: 15000000 INFO @ Mon, 29 Jun 2020 21:51:29: 3000000 INFO @ Mon, 29 Jun 2020 21:51:29: 9000000 INFO @ Mon, 29 Jun 2020 21:51:32: 16000000 INFO @ Mon, 29 Jun 2020 21:51:33: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:51:33: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:51:33: #1 total tags in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:51:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:51:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:51:33: #1 tags after filtering in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:51:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:51:33: #1 finished! INFO @ Mon, 29 Jun 2020 21:51:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:51:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:51:34: 4000000 INFO @ Mon, 29 Jun 2020 21:51:34: #2 number of paired peaks: 12 WARNING @ Mon, 29 Jun 2020 21:51:34: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:51:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:51:35: 10000000 INFO @ Mon, 29 Jun 2020 21:51:39: 5000000 INFO @ Mon, 29 Jun 2020 21:51:39: 11000000 INFO @ Mon, 29 Jun 2020 21:51:44: 6000000 INFO @ Mon, 29 Jun 2020 21:51:44: 12000000 INFO @ Mon, 29 Jun 2020 21:51:49: 7000000 INFO @ Mon, 29 Jun 2020 21:51:49: 13000000 INFO @ Mon, 29 Jun 2020 21:51:54: 8000000 INFO @ Mon, 29 Jun 2020 21:51:54: 14000000 INFO @ Mon, 29 Jun 2020 21:51:59: 9000000 INFO @ Mon, 29 Jun 2020 21:51:59: 15000000 INFO @ Mon, 29 Jun 2020 21:52:04: 10000000 INFO @ Mon, 29 Jun 2020 21:52:04: 16000000 INFO @ Mon, 29 Jun 2020 21:52:05: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:52:05: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:52:05: #1 total tags in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:52:05: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:52:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:52:06: #1 tags after filtering in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:52:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:52:06: #1 finished! INFO @ Mon, 29 Jun 2020 21:52:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:52:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:52:07: #2 number of paired peaks: 12 WARNING @ Mon, 29 Jun 2020 21:52:07: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:52:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:52:08: 11000000 INFO @ Mon, 29 Jun 2020 21:52:13: 12000000 INFO @ Mon, 29 Jun 2020 21:52:18: 13000000 INFO @ Mon, 29 Jun 2020 21:52:23: 14000000 INFO @ Mon, 29 Jun 2020 21:52:27: 15000000 INFO @ Mon, 29 Jun 2020 21:52:32: 16000000 INFO @ Mon, 29 Jun 2020 21:52:33: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:52:33: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:52:33: #1 total tags in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:52:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:52:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:52:33: #1 tags after filtering in treatment: 16164835 INFO @ Mon, 29 Jun 2020 21:52:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:52:33: #1 finished! INFO @ Mon, 29 Jun 2020 21:52:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:52:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:52:34: #2 number of paired peaks: 12 WARNING @ Mon, 29 Jun 2020 21:52:34: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:52:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104975/SRX104975.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。