Job ID = 6527531 SRX = SRX104970 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:23:51 prefetch.2.10.7: 1) Downloading 'SRR363414'... 2020-06-29T12:23:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:25:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:25:42 prefetch.2.10.7: 'SRR363414' is valid 2020-06-29T12:25:42 prefetch.2.10.7: 1) 'SRR363414' was downloaded successfully Read 21379916 spots for SRR363414/SRR363414.sra Written 21379916 spots for SRR363414/SRR363414.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:41 21379916 reads; of these: 21379916 (100.00%) were unpaired; of these: 694948 (3.25%) aligned 0 times 16843882 (78.78%) aligned exactly 1 time 3841086 (17.97%) aligned >1 times 96.75% overall alignment rate Time searching: 00:05:41 Overall time: 00:05:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2907050 / 20684968 = 0.1405 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:42:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:42:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:42:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:42:19: 1000000 INFO @ Mon, 29 Jun 2020 21:42:25: 2000000 INFO @ Mon, 29 Jun 2020 21:42:30: 3000000 INFO @ Mon, 29 Jun 2020 21:42:35: 4000000 INFO @ Mon, 29 Jun 2020 21:42:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:42:44: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:42:44: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:42:46: 6000000 INFO @ Mon, 29 Jun 2020 21:42:50: 1000000 INFO @ Mon, 29 Jun 2020 21:42:52: 7000000 INFO @ Mon, 29 Jun 2020 21:42:55: 2000000 INFO @ Mon, 29 Jun 2020 21:42:57: 8000000 INFO @ Mon, 29 Jun 2020 21:43:01: 3000000 INFO @ Mon, 29 Jun 2020 21:43:03: 9000000 INFO @ Mon, 29 Jun 2020 21:43:07: 4000000 INFO @ Mon, 29 Jun 2020 21:43:09: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 21:43:12: 5000000 INFO @ Mon, 29 Jun 2020 21:43:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 21:43:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 21:43:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 21:43:14: 11000000 INFO @ Mon, 29 Jun 2020 21:43:18: 6000000 INFO @ Mon, 29 Jun 2020 21:43:19: 1000000 INFO @ Mon, 29 Jun 2020 21:43:20: 12000000 INFO @ Mon, 29 Jun 2020 21:43:23: 7000000 INFO @ Mon, 29 Jun 2020 21:43:24: 2000000 INFO @ Mon, 29 Jun 2020 21:43:25: 13000000 INFO @ Mon, 29 Jun 2020 21:43:29: 8000000 INFO @ Mon, 29 Jun 2020 21:43:29: 3000000 INFO @ Mon, 29 Jun 2020 21:43:31: 14000000 INFO @ Mon, 29 Jun 2020 21:43:35: 4000000 INFO @ Mon, 29 Jun 2020 21:43:35: 9000000 INFO @ Mon, 29 Jun 2020 21:43:37: 15000000 INFO @ Mon, 29 Jun 2020 21:43:40: 5000000 INFO @ Mon, 29 Jun 2020 21:43:40: 10000000 INFO @ Mon, 29 Jun 2020 21:43:43: 16000000 INFO @ Mon, 29 Jun 2020 21:43:45: 6000000 INFO @ Mon, 29 Jun 2020 21:43:46: 11000000 INFO @ Mon, 29 Jun 2020 21:43:48: 17000000 INFO @ Mon, 29 Jun 2020 21:43:50: 7000000 INFO @ Mon, 29 Jun 2020 21:43:52: 12000000 INFO @ Mon, 29 Jun 2020 21:43:53: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:43:53: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:43:53: #1 total tags in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:43:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:43:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:43:54: #1 tags after filtering in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:43:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:43:54: #1 finished! INFO @ Mon, 29 Jun 2020 21:43:54: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:43:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:43:55: #2 number of paired peaks: 14 WARNING @ Mon, 29 Jun 2020 21:43:55: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:43:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:43:55: 8000000 INFO @ Mon, 29 Jun 2020 21:43:58: 13000000 INFO @ Mon, 29 Jun 2020 21:44:00: 9000000 INFO @ Mon, 29 Jun 2020 21:44:03: 14000000 INFO @ Mon, 29 Jun 2020 21:44:05: 10000000 INFO @ Mon, 29 Jun 2020 21:44:09: 15000000 INFO @ Mon, 29 Jun 2020 21:44:11: 11000000 INFO @ Mon, 29 Jun 2020 21:44:15: 16000000 INFO @ Mon, 29 Jun 2020 21:44:16: 12000000 INFO @ Mon, 29 Jun 2020 21:44:21: 17000000 INFO @ Mon, 29 Jun 2020 21:44:22: 13000000 INFO @ Mon, 29 Jun 2020 21:44:26: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:44:26: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:44:26: #1 total tags in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:44:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:44:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:44:26: #1 tags after filtering in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:44:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:44:26: #1 finished! INFO @ Mon, 29 Jun 2020 21:44:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:44:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:44:27: #2 number of paired peaks: 14 WARNING @ Mon, 29 Jun 2020 21:44:27: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 21:44:27: 14000000 INFO @ Mon, 29 Jun 2020 21:44:33: 15000000 INFO @ Mon, 29 Jun 2020 21:44:39: 16000000 INFO @ Mon, 29 Jun 2020 21:44:44: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 21:44:49: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 21:44:49: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 21:44:49: #1 total tags in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:44:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 21:44:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 21:44:49: #1 tags after filtering in treatment: 17777918 INFO @ Mon, 29 Jun 2020 21:44:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 21:44:49: #1 finished! INFO @ Mon, 29 Jun 2020 21:44:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 21:44:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 21:44:51: #2 number of paired peaks: 14 WARNING @ Mon, 29 Jun 2020 21:44:51: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 21:44:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX104970/SRX104970.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。