Job ID = 16440035 SRX = SRX10447590 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 33768119 spots for SRR14073305/SRR14073305.sra Written 33768119 spots for SRR14073305/SRR14073305.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440740 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:56:38 33768119 reads; of these: 33768119 (100.00%) were paired; of these: 4049208 (11.99%) aligned concordantly 0 times 25499146 (75.51%) aligned concordantly exactly 1 time 4219765 (12.50%) aligned concordantly >1 times ---- 4049208 pairs aligned concordantly 0 times; of these: 437403 (10.80%) aligned discordantly 1 time ---- 3611805 pairs aligned 0 times concordantly or discordantly; of these: 7223610 mates make up the pairs; of these: 6589940 (91.23%) aligned 0 times 455743 (6.31%) aligned exactly 1 time 177927 (2.46%) aligned >1 times 90.24% overall alignment rate Time searching: 00:56:39 Overall time: 00:56:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3263373 / 30105449 = 0.1084 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:07:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:07:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:07:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:07:41: 1000000 INFO @ Tue, 02 Aug 2022 18:07:50: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:07:58: 3000000 INFO @ Tue, 02 Aug 2022 18:08:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:08:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:08:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:08:07: 4000000 INFO @ Tue, 02 Aug 2022 18:08:08: 1000000 INFO @ Tue, 02 Aug 2022 18:08:16: 5000000 INFO @ Tue, 02 Aug 2022 18:08:17: 2000000 INFO @ Tue, 02 Aug 2022 18:08:26: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:08:28: 3000000 INFO @ Tue, 02 Aug 2022 18:08:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:08:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:08:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:08:35: 7000000 INFO @ Tue, 02 Aug 2022 18:08:37: 4000000 INFO @ Tue, 02 Aug 2022 18:08:39: 1000000 INFO @ Tue, 02 Aug 2022 18:08:45: 8000000 INFO @ Tue, 02 Aug 2022 18:08:46: 5000000 INFO @ Tue, 02 Aug 2022 18:08:48: 2000000 INFO @ Tue, 02 Aug 2022 18:08:54: 9000000 INFO @ Tue, 02 Aug 2022 18:08:55: 6000000 INFO @ Tue, 02 Aug 2022 18:08:57: 3000000 INFO @ Tue, 02 Aug 2022 18:09:03: 10000000 INFO @ Tue, 02 Aug 2022 18:09:04: 7000000 INFO @ Tue, 02 Aug 2022 18:09:05: 4000000 INFO @ Tue, 02 Aug 2022 18:09:13: 8000000 INFO @ Tue, 02 Aug 2022 18:09:13: 11000000 INFO @ Tue, 02 Aug 2022 18:09:14: 5000000 INFO @ Tue, 02 Aug 2022 18:09:22: 9000000 INFO @ Tue, 02 Aug 2022 18:09:22: 6000000 INFO @ Tue, 02 Aug 2022 18:09:23: 12000000 INFO @ Tue, 02 Aug 2022 18:09:30: 10000000 INFO @ Tue, 02 Aug 2022 18:09:31: 7000000 INFO @ Tue, 02 Aug 2022 18:09:32: 13000000 INFO @ Tue, 02 Aug 2022 18:09:39: 11000000 INFO @ Tue, 02 Aug 2022 18:09:40: 8000000 INFO @ Tue, 02 Aug 2022 18:09:42: 14000000 INFO @ Tue, 02 Aug 2022 18:09:48: 12000000 INFO @ Tue, 02 Aug 2022 18:09:49: 9000000 INFO @ Tue, 02 Aug 2022 18:09:51: 15000000 INFO @ Tue, 02 Aug 2022 18:09:56: 13000000 INFO @ Tue, 02 Aug 2022 18:09:58: 10000000 INFO @ Tue, 02 Aug 2022 18:10:00: 16000000 INFO @ Tue, 02 Aug 2022 18:10:05: 14000000 INFO @ Tue, 02 Aug 2022 18:10:08: 11000000 INFO @ Tue, 02 Aug 2022 18:10:10: 17000000 INFO @ Tue, 02 Aug 2022 18:10:14: 15000000 INFO @ Tue, 02 Aug 2022 18:10:18: 12000000 INFO @ Tue, 02 Aug 2022 18:10:19: 18000000 INFO @ Tue, 02 Aug 2022 18:10:22: 16000000 INFO @ Tue, 02 Aug 2022 18:10:27: 13000000 INFO @ Tue, 02 Aug 2022 18:10:29: 19000000 INFO @ Tue, 02 Aug 2022 18:10:31: 17000000 INFO @ Tue, 02 Aug 2022 18:10:36: 14000000 INFO @ Tue, 02 Aug 2022 18:10:38: 20000000 INFO @ Tue, 02 Aug 2022 18:10:39: 18000000 INFO @ Tue, 02 Aug 2022 18:10:45: 15000000 INFO @ Tue, 02 Aug 2022 18:10:48: 21000000 INFO @ Tue, 02 Aug 2022 18:10:48: 19000000 INFO @ Tue, 02 Aug 2022 18:10:54: 16000000 INFO @ Tue, 02 Aug 2022 18:10:56: 20000000 INFO @ Tue, 02 Aug 2022 18:10:57: 22000000 INFO @ Tue, 02 Aug 2022 18:11:03: 17000000 INFO @ Tue, 02 Aug 2022 18:11:04: 21000000 INFO @ Tue, 02 Aug 2022 18:11:06: 23000000 INFO @ Tue, 02 Aug 2022 18:11:12: 18000000 INFO @ Tue, 02 Aug 2022 18:11:12: 22000000 INFO @ Tue, 02 Aug 2022 18:11:15: 24000000 INFO @ Tue, 02 Aug 2022 18:11:20: 19000000 INFO @ Tue, 02 Aug 2022 18:11:21: 23000000 INFO @ Tue, 02 Aug 2022 18:11:24: 25000000 INFO @ Tue, 02 Aug 2022 18:11:28: 24000000 INFO @ Tue, 02 Aug 2022 18:11:29: 20000000 INFO @ Tue, 02 Aug 2022 18:11:33: 26000000 INFO @ Tue, 02 Aug 2022 18:11:36: 25000000 INFO @ Tue, 02 Aug 2022 18:11:37: 21000000 INFO @ Tue, 02 Aug 2022 18:11:41: 27000000 INFO @ Tue, 02 Aug 2022 18:11:44: 26000000 INFO @ Tue, 02 Aug 2022 18:11:45: 22000000 INFO @ Tue, 02 Aug 2022 18:11:50: 28000000 INFO @ Tue, 02 Aug 2022 18:11:51: 27000000 INFO @ Tue, 02 Aug 2022 18:11:53: 23000000 INFO @ Tue, 02 Aug 2022 18:11:59: 29000000 INFO @ Tue, 02 Aug 2022 18:11:59: 28000000 INFO @ Tue, 02 Aug 2022 18:12:02: 24000000 INFO @ Tue, 02 Aug 2022 18:12:07: 29000000 INFO @ Tue, 02 Aug 2022 18:12:08: 30000000 INFO @ Tue, 02 Aug 2022 18:12:10: 25000000 INFO @ Tue, 02 Aug 2022 18:12:15: 30000000 INFO @ Tue, 02 Aug 2022 18:12:17: 31000000 INFO @ Tue, 02 Aug 2022 18:12:18: 26000000 INFO @ Tue, 02 Aug 2022 18:12:23: 31000000 INFO @ Tue, 02 Aug 2022 18:12:26: 32000000 INFO @ Tue, 02 Aug 2022 18:12:26: 27000000 INFO @ Tue, 02 Aug 2022 18:12:31: 32000000 INFO @ Tue, 02 Aug 2022 18:12:34: 33000000 INFO @ Tue, 02 Aug 2022 18:12:35: 28000000 INFO @ Tue, 02 Aug 2022 18:12:39: 33000000 INFO @ Tue, 02 Aug 2022 18:12:43: 34000000 INFO @ Tue, 02 Aug 2022 18:12:43: 29000000 INFO @ Tue, 02 Aug 2022 18:12:47: 34000000 INFO @ Tue, 02 Aug 2022 18:12:51: 35000000 INFO @ Tue, 02 Aug 2022 18:12:52: 30000000 INFO @ Tue, 02 Aug 2022 18:12:56: 35000000 INFO @ Tue, 02 Aug 2022 18:13:00: 36000000 INFO @ Tue, 02 Aug 2022 18:13:00: 31000000 INFO @ Tue, 02 Aug 2022 18:13:05: 36000000 INFO @ Tue, 02 Aug 2022 18:13:08: 37000000 INFO @ Tue, 02 Aug 2022 18:13:09: 32000000 INFO @ Tue, 02 Aug 2022 18:13:13: 37000000 INFO @ Tue, 02 Aug 2022 18:13:17: 38000000 INFO @ Tue, 02 Aug 2022 18:13:17: 33000000 INFO @ Tue, 02 Aug 2022 18:13:21: 38000000 INFO @ Tue, 02 Aug 2022 18:13:24: 39000000 INFO @ Tue, 02 Aug 2022 18:13:26: 34000000 INFO @ Tue, 02 Aug 2022 18:13:29: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:13:32: 40000000 INFO @ Tue, 02 Aug 2022 18:13:34: 35000000 INFO @ Tue, 02 Aug 2022 18:13:37: 40000000 INFO @ Tue, 02 Aug 2022 18:13:40: 41000000 INFO @ Tue, 02 Aug 2022 18:13:43: 36000000 INFO @ Tue, 02 Aug 2022 18:13:45: 41000000 INFO @ Tue, 02 Aug 2022 18:13:48: 42000000 INFO @ Tue, 02 Aug 2022 18:13:51: 37000000 INFO @ Tue, 02 Aug 2022 18:13:53: 42000000 INFO @ Tue, 02 Aug 2022 18:13:56: 43000000 INFO @ Tue, 02 Aug 2022 18:13:59: 38000000 INFO @ Tue, 02 Aug 2022 18:14:01: 43000000 INFO @ Tue, 02 Aug 2022 18:14:05: 44000000 INFO @ Tue, 02 Aug 2022 18:14:07: 39000000 INFO @ Tue, 02 Aug 2022 18:14:10: 44000000 INFO @ Tue, 02 Aug 2022 18:14:13: 45000000 INFO @ Tue, 02 Aug 2022 18:14:16: 40000000 INFO @ Tue, 02 Aug 2022 18:14:19: 45000000 INFO @ Tue, 02 Aug 2022 18:14:22: 46000000 INFO @ Tue, 02 Aug 2022 18:14:24: 41000000 INFO @ Tue, 02 Aug 2022 18:14:27: 46000000 INFO @ Tue, 02 Aug 2022 18:14:30: 47000000 INFO @ Tue, 02 Aug 2022 18:14:32: 42000000 INFO @ Tue, 02 Aug 2022 18:14:35: 47000000 INFO @ Tue, 02 Aug 2022 18:14:38: 48000000 INFO @ Tue, 02 Aug 2022 18:14:40: 43000000 INFO @ Tue, 02 Aug 2022 18:14:43: 48000000 INFO @ Tue, 02 Aug 2022 18:14:46: 49000000 INFO @ Tue, 02 Aug 2022 18:14:49: 44000000 INFO @ Tue, 02 Aug 2022 18:14:51: 49000000 INFO @ Tue, 02 Aug 2022 18:14:54: 50000000 INFO @ Tue, 02 Aug 2022 18:14:57: 45000000 INFO @ Tue, 02 Aug 2022 18:15:00: 50000000 INFO @ Tue, 02 Aug 2022 18:15:03: 51000000 INFO @ Tue, 02 Aug 2022 18:15:06: 46000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:15:09: 51000000 INFO @ Tue, 02 Aug 2022 18:15:12: 52000000 INFO @ Tue, 02 Aug 2022 18:15:14: 47000000 INFO @ Tue, 02 Aug 2022 18:15:17: 52000000 INFO @ Tue, 02 Aug 2022 18:15:21: 53000000 INFO @ Tue, 02 Aug 2022 18:15:22: 48000000 INFO @ Tue, 02 Aug 2022 18:15:26: 53000000 INFO @ Tue, 02 Aug 2022 18:15:29: 54000000 INFO @ Tue, 02 Aug 2022 18:15:30: 49000000 INFO @ Tue, 02 Aug 2022 18:15:33: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:15:33: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:15:33: #1 total tags in treatment: 26478721 INFO @ Tue, 02 Aug 2022 18:15:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:15:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:15:34: #1 tags after filtering in treatment: 17045769 INFO @ Tue, 02 Aug 2022 18:15:34: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:15:34: #1 finished! INFO @ Tue, 02 Aug 2022 18:15:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:15:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:15:35: 54000000 INFO @ Tue, 02 Aug 2022 18:15:35: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:15:35: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:15:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:15:38: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:15:38: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:15:38: #1 total tags in treatment: 26478721 INFO @ Tue, 02 Aug 2022 18:15:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:15:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:15:39: 50000000 INFO @ Tue, 02 Aug 2022 18:15:39: #1 tags after filtering in treatment: 17045769 INFO @ Tue, 02 Aug 2022 18:15:39: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:15:39: #1 finished! INFO @ Tue, 02 Aug 2022 18:15:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:15:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:15:41: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:15:41: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:15:47: 51000000 INFO @ Tue, 02 Aug 2022 18:15:56: 52000000 INFO @ Tue, 02 Aug 2022 18:16:04: 53000000 INFO @ Tue, 02 Aug 2022 18:16:13: 54000000 INFO @ Tue, 02 Aug 2022 18:16:16: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:16:16: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:16:16: #1 total tags in treatment: 26478721 INFO @ Tue, 02 Aug 2022 18:16:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:16:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:16:17: #1 tags after filtering in treatment: 17045769 INFO @ Tue, 02 Aug 2022 18:16:17: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:16:17: #1 finished! INFO @ Tue, 02 Aug 2022 18:16:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:16:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:16:19: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:16:19: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:16:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447590/SRX10447590.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling