Job ID = 14167077 SRX = SRX10412059 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36208292 spots for SRR14035723/SRR14035723.sra Written 36208292 spots for SRR14035723/SRR14035723.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168081 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:47:40 36208292 reads; of these: 36208292 (100.00%) were paired; of these: 3801727 (10.50%) aligned concordantly 0 times 22204799 (61.33%) aligned concordantly exactly 1 time 10201766 (28.18%) aligned concordantly >1 times ---- 3801727 pairs aligned concordantly 0 times; of these: 601354 (15.82%) aligned discordantly 1 time ---- 3200373 pairs aligned 0 times concordantly or discordantly; of these: 6400746 mates make up the pairs; of these: 4517996 (70.59%) aligned 0 times 657037 (10.27%) aligned exactly 1 time 1225713 (19.15%) aligned >1 times 93.76% overall alignment rate Time searching: 02:47:40 Overall time: 02:47:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7566273 / 32927269 = 0.2298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:58:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:58:28: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:58:28: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:58:38: 1000000 INFO @ Fri, 10 Dec 2021 12:58:49: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:58:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:58:58: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:58:58: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:58:59: 3000000 INFO @ Fri, 10 Dec 2021 12:59:08: 1000000 INFO @ Fri, 10 Dec 2021 12:59:11: 4000000 INFO @ Fri, 10 Dec 2021 12:59:18: 2000000 INFO @ Fri, 10 Dec 2021 12:59:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:59:28: 3000000 INFO @ Fri, 10 Dec 2021 12:59:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:59:28: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:59:28: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:59:34: 6000000 INFO @ Fri, 10 Dec 2021 12:59:39: 1000000 INFO @ Fri, 10 Dec 2021 12:59:39: 4000000 INFO @ Fri, 10 Dec 2021 12:59:46: 7000000 INFO @ Fri, 10 Dec 2021 12:59:49: 2000000 INFO @ Fri, 10 Dec 2021 12:59:50: 5000000 INFO @ Fri, 10 Dec 2021 12:59:58: 8000000 INFO @ Fri, 10 Dec 2021 13:00:00: 3000000 INFO @ Fri, 10 Dec 2021 13:00:00: 6000000 INFO @ Fri, 10 Dec 2021 13:00:09: 9000000 INFO @ Fri, 10 Dec 2021 13:00:11: 4000000 INFO @ Fri, 10 Dec 2021 13:00:11: 7000000 INFO @ Fri, 10 Dec 2021 13:00:21: 10000000 INFO @ Fri, 10 Dec 2021 13:00:22: 5000000 INFO @ Fri, 10 Dec 2021 13:00:22: 8000000 INFO @ Fri, 10 Dec 2021 13:00:32: 6000000 INFO @ Fri, 10 Dec 2021 13:00:33: 9000000 INFO @ Fri, 10 Dec 2021 13:00:33: 11000000 INFO @ Fri, 10 Dec 2021 13:00:43: 7000000 INFO @ Fri, 10 Dec 2021 13:00:43: 10000000 INFO @ Fri, 10 Dec 2021 13:00:45: 12000000 INFO @ Fri, 10 Dec 2021 13:00:54: 8000000 INFO @ Fri, 10 Dec 2021 13:00:54: 11000000 INFO @ Fri, 10 Dec 2021 13:00:56: 13000000 INFO @ Fri, 10 Dec 2021 13:01:04: 9000000 INFO @ Fri, 10 Dec 2021 13:01:05: 12000000 INFO @ Fri, 10 Dec 2021 13:01:08: 14000000 INFO @ Fri, 10 Dec 2021 13:01:15: 10000000 INFO @ Fri, 10 Dec 2021 13:01:15: 13000000 INFO @ Fri, 10 Dec 2021 13:01:20: 15000000 INFO @ Fri, 10 Dec 2021 13:01:26: 11000000 INFO @ Fri, 10 Dec 2021 13:01:26: 14000000 INFO @ Fri, 10 Dec 2021 13:01:31: 16000000 INFO @ Fri, 10 Dec 2021 13:01:37: 12000000 INFO @ Fri, 10 Dec 2021 13:01:37: 15000000 INFO @ Fri, 10 Dec 2021 13:01:43: 17000000 INFO @ Fri, 10 Dec 2021 13:01:47: 13000000 INFO @ Fri, 10 Dec 2021 13:01:48: 16000000 INFO @ Fri, 10 Dec 2021 13:01:55: 18000000 INFO @ Fri, 10 Dec 2021 13:01:58: 14000000 INFO @ Fri, 10 Dec 2021 13:01:58: 17000000 INFO @ Fri, 10 Dec 2021 13:02:07: 19000000 INFO @ Fri, 10 Dec 2021 13:02:09: 15000000 INFO @ Fri, 10 Dec 2021 13:02:09: 18000000 INFO @ Fri, 10 Dec 2021 13:02:18: 20000000 INFO @ Fri, 10 Dec 2021 13:02:20: 16000000 INFO @ Fri, 10 Dec 2021 13:02:20: 19000000 INFO @ Fri, 10 Dec 2021 13:02:30: 21000000 INFO @ Fri, 10 Dec 2021 13:02:30: 17000000 INFO @ Fri, 10 Dec 2021 13:02:31: 20000000 INFO @ Fri, 10 Dec 2021 13:02:41: 18000000 INFO @ Fri, 10 Dec 2021 13:02:42: 21000000 INFO @ Fri, 10 Dec 2021 13:02:42: 22000000 INFO @ Fri, 10 Dec 2021 13:02:52: 19000000 INFO @ Fri, 10 Dec 2021 13:02:52: 22000000 INFO @ Fri, 10 Dec 2021 13:02:54: 23000000 INFO @ Fri, 10 Dec 2021 13:03:03: 20000000 INFO @ Fri, 10 Dec 2021 13:03:03: 23000000 INFO @ Fri, 10 Dec 2021 13:03:05: 24000000 INFO @ Fri, 10 Dec 2021 13:03:14: 21000000 INFO @ Fri, 10 Dec 2021 13:03:14: 24000000 INFO @ Fri, 10 Dec 2021 13:03:17: 25000000 INFO @ Fri, 10 Dec 2021 13:03:24: 22000000 INFO @ Fri, 10 Dec 2021 13:03:24: 25000000 INFO @ Fri, 10 Dec 2021 13:03:28: 26000000 INFO @ Fri, 10 Dec 2021 13:03:35: 23000000 INFO @ Fri, 10 Dec 2021 13:03:35: 26000000 INFO @ Fri, 10 Dec 2021 13:03:40: 27000000 INFO @ Fri, 10 Dec 2021 13:03:46: 24000000 INFO @ Fri, 10 Dec 2021 13:03:46: 27000000 INFO @ Fri, 10 Dec 2021 13:03:51: 28000000 INFO @ Fri, 10 Dec 2021 13:03:56: 25000000 INFO @ Fri, 10 Dec 2021 13:03:56: 28000000 INFO @ Fri, 10 Dec 2021 13:04:02: 29000000 INFO @ Fri, 10 Dec 2021 13:04:07: 26000000 INFO @ Fri, 10 Dec 2021 13:04:07: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:04:14: 30000000 INFO @ Fri, 10 Dec 2021 13:04:17: 27000000 INFO @ Fri, 10 Dec 2021 13:04:18: 30000000 INFO @ Fri, 10 Dec 2021 13:04:25: 31000000 INFO @ Fri, 10 Dec 2021 13:04:27: 28000000 INFO @ Fri, 10 Dec 2021 13:04:29: 31000000 INFO @ Fri, 10 Dec 2021 13:04:37: 32000000 INFO @ Fri, 10 Dec 2021 13:04:38: 29000000 INFO @ Fri, 10 Dec 2021 13:04:39: 32000000 INFO @ Fri, 10 Dec 2021 13:04:48: 30000000 INFO @ Fri, 10 Dec 2021 13:04:48: 33000000 INFO @ Fri, 10 Dec 2021 13:04:50: 33000000 INFO @ Fri, 10 Dec 2021 13:04:58: 31000000 INFO @ Fri, 10 Dec 2021 13:05:00: 34000000 INFO @ Fri, 10 Dec 2021 13:05:01: 34000000 INFO @ Fri, 10 Dec 2021 13:05:08: 32000000 INFO @ Fri, 10 Dec 2021 13:05:12: 35000000 INFO @ Fri, 10 Dec 2021 13:05:13: 35000000 INFO @ Fri, 10 Dec 2021 13:05:18: 33000000 INFO @ Fri, 10 Dec 2021 13:05:24: 36000000 INFO @ Fri, 10 Dec 2021 13:05:25: 36000000 INFO @ Fri, 10 Dec 2021 13:05:28: 34000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:05:36: 37000000 INFO @ Fri, 10 Dec 2021 13:05:37: 37000000 INFO @ Fri, 10 Dec 2021 13:05:38: 35000000 INFO @ Fri, 10 Dec 2021 13:05:48: 38000000 INFO @ Fri, 10 Dec 2021 13:05:49: 36000000 INFO @ Fri, 10 Dec 2021 13:05:49: 38000000 INFO @ Fri, 10 Dec 2021 13:05:59: 37000000 INFO @ Fri, 10 Dec 2021 13:06:00: 39000000 INFO @ Fri, 10 Dec 2021 13:06:01: 39000000 INFO @ Fri, 10 Dec 2021 13:06:10: 38000000 INFO @ Fri, 10 Dec 2021 13:06:12: 40000000 INFO @ Fri, 10 Dec 2021 13:06:13: 40000000 INFO @ Fri, 10 Dec 2021 13:06:20: 39000000 INFO @ Fri, 10 Dec 2021 13:06:24: 41000000 INFO @ Fri, 10 Dec 2021 13:06:25: 41000000 INFO @ Fri, 10 Dec 2021 13:06:31: 40000000 INFO @ Fri, 10 Dec 2021 13:06:36: 42000000 INFO @ Fri, 10 Dec 2021 13:06:37: 42000000 INFO @ Fri, 10 Dec 2021 13:06:41: 41000000 INFO @ Fri, 10 Dec 2021 13:06:48: 43000000 INFO @ Fri, 10 Dec 2021 13:06:49: 43000000 INFO @ Fri, 10 Dec 2021 13:06:52: 42000000 INFO @ Fri, 10 Dec 2021 13:07:00: 44000000 INFO @ Fri, 10 Dec 2021 13:07:01: 44000000 INFO @ Fri, 10 Dec 2021 13:07:02: 43000000 INFO @ Fri, 10 Dec 2021 13:07:12: 45000000 INFO @ Fri, 10 Dec 2021 13:07:12: 44000000 INFO @ Fri, 10 Dec 2021 13:07:13: 45000000 INFO @ Fri, 10 Dec 2021 13:07:23: 45000000 INFO @ Fri, 10 Dec 2021 13:07:23: 46000000 INFO @ Fri, 10 Dec 2021 13:07:24: 46000000 INFO @ Fri, 10 Dec 2021 13:07:33: 46000000 INFO @ Fri, 10 Dec 2021 13:07:35: 47000000 INFO @ Fri, 10 Dec 2021 13:07:36: 47000000 INFO @ Fri, 10 Dec 2021 13:07:43: 47000000 INFO @ Fri, 10 Dec 2021 13:07:47: 48000000 INFO @ Fri, 10 Dec 2021 13:07:47: 48000000 INFO @ Fri, 10 Dec 2021 13:07:54: 48000000 INFO @ Fri, 10 Dec 2021 13:07:59: 49000000 INFO @ Fri, 10 Dec 2021 13:07:59: 49000000 INFO @ Fri, 10 Dec 2021 13:08:04: 49000000 INFO @ Fri, 10 Dec 2021 13:08:10: 50000000 INFO @ Fri, 10 Dec 2021 13:08:11: 50000000 INFO @ Fri, 10 Dec 2021 13:08:15: 50000000 INFO @ Fri, 10 Dec 2021 13:08:21: 51000000 INFO @ Fri, 10 Dec 2021 13:08:22: 51000000 INFO @ Fri, 10 Dec 2021 13:08:25: 51000000 INFO @ Fri, 10 Dec 2021 13:08:33: 52000000 INFO @ Fri, 10 Dec 2021 13:08:33: 52000000 INFO @ Fri, 10 Dec 2021 13:08:35: 52000000 INFO @ Fri, 10 Dec 2021 13:08:41: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:08:41: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:08:41: #1 total tags in treatment: 24941752 INFO @ Fri, 10 Dec 2021 13:08:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:08:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:08:42: #1 tags after filtering in treatment: 22540049 INFO @ Fri, 10 Dec 2021 13:08:42: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:08:42: #1 finished! INFO @ Fri, 10 Dec 2021 13:08:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:08:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:08:42: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:08:42: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:08:42: #1 total tags in treatment: 24941752 INFO @ Fri, 10 Dec 2021 13:08:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:08:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:08:43: #1 tags after filtering in treatment: 22540049 INFO @ Fri, 10 Dec 2021 13:08:43: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:08:43: #1 finished! INFO @ Fri, 10 Dec 2021 13:08:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:08:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:08:43: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:08:43: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:08:43: #1 total tags in treatment: 24941752 INFO @ Fri, 10 Dec 2021 13:08:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:08:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:08:43: #2 number of paired peaks: 111 WARNING @ Fri, 10 Dec 2021 13:08:43: Fewer paired peaks (111) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 111 pairs to build model! INFO @ Fri, 10 Dec 2021 13:08:43: start model_add_line... INFO @ Fri, 10 Dec 2021 13:08:43: start X-correlation... INFO @ Fri, 10 Dec 2021 13:08:43: end of X-cor INFO @ Fri, 10 Dec 2021 13:08:43: #2 finished! INFO @ Fri, 10 Dec 2021 13:08:43: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 13:08:43: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 13:08:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05_model.r WARNING @ Fri, 10 Dec 2021 13:08:43: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:08:43: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Fri, 10 Dec 2021 13:08:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:08:43: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:08:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:08:44: #1 tags after filtering in treatment: 22540049 INFO @ Fri, 10 Dec 2021 13:08:44: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:08:44: #1 finished! INFO @ Fri, 10 Dec 2021 13:08:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:08:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:08:44: #2 number of paired peaks: 111 WARNING @ Fri, 10 Dec 2021 13:08:44: Fewer paired peaks (111) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 111 pairs to build model! INFO @ Fri, 10 Dec 2021 13:08:44: start model_add_line... INFO @ Fri, 10 Dec 2021 13:08:44: start X-correlation... INFO @ Fri, 10 Dec 2021 13:08:44: end of X-cor INFO @ Fri, 10 Dec 2021 13:08:44: #2 finished! INFO @ Fri, 10 Dec 2021 13:08:44: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 13:08:44: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 13:08:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10_model.r WARNING @ Fri, 10 Dec 2021 13:08:44: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:08:44: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Fri, 10 Dec 2021 13:08:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:08:44: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:08:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:08:45: #2 number of paired peaks: 111 WARNING @ Fri, 10 Dec 2021 13:08:45: Fewer paired peaks (111) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 111 pairs to build model! INFO @ Fri, 10 Dec 2021 13:08:45: start model_add_line... INFO @ Fri, 10 Dec 2021 13:08:45: start X-correlation... INFO @ Fri, 10 Dec 2021 13:08:45: end of X-cor INFO @ Fri, 10 Dec 2021 13:08:45: #2 finished! INFO @ Fri, 10 Dec 2021 13:08:45: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 13:08:45: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 13:08:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20_model.r WARNING @ Fri, 10 Dec 2021 13:08:45: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:08:45: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Fri, 10 Dec 2021 13:08:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:08:45: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:08:45: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:09:24: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:09:25: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:09:26: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:09:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:09:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:09:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.05_summits.bed INFO @ Fri, 10 Dec 2021 13:09:44: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (7194 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.20_summits.bed INFO @ Fri, 10 Dec 2021 13:09:46: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2119 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:09:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412059/SRX10412059.10_summits.bed INFO @ Fri, 10 Dec 2021 13:09:46: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (4506 records, 4 fields): 5 millis CompletedMACS2peakCalling