Job ID = 14171833 SRX = SRX10386246 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20823071 spots for SRR14009311/SRR14009311.sra Written 20823071 spots for SRR14009311/SRR14009311.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172440 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:05 20823071 reads; of these: 20823071 (100.00%) were paired; of these: 2553779 (12.26%) aligned concordantly 0 times 14227871 (68.33%) aligned concordantly exactly 1 time 4041421 (19.41%) aligned concordantly >1 times ---- 2553779 pairs aligned concordantly 0 times; of these: 657662 (25.75%) aligned discordantly 1 time ---- 1896117 pairs aligned 0 times concordantly or discordantly; of these: 3792234 mates make up the pairs; of these: 2418347 (63.77%) aligned 0 times 697718 (18.40%) aligned exactly 1 time 676169 (17.83%) aligned >1 times 94.19% overall alignment rate Time searching: 00:33:06 Overall time: 00:33:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2714943 / 18891227 = 0.1437 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:47:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:47:46: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:47:46: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:47:50: 1000000 INFO @ Sat, 11 Dec 2021 13:47:55: 2000000 INFO @ Sat, 11 Dec 2021 13:47:59: 3000000 INFO @ Sat, 11 Dec 2021 13:48:04: 4000000 INFO @ Sat, 11 Dec 2021 13:48:08: 5000000 INFO @ Sat, 11 Dec 2021 13:48:13: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:48:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:48:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:48:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:48:17: 7000000 INFO @ Sat, 11 Dec 2021 13:48:20: 1000000 INFO @ Sat, 11 Dec 2021 13:48:21: 8000000 INFO @ Sat, 11 Dec 2021 13:48:24: 2000000 INFO @ Sat, 11 Dec 2021 13:48:26: 9000000 INFO @ Sat, 11 Dec 2021 13:48:29: 3000000 INFO @ Sat, 11 Dec 2021 13:48:30: 10000000 INFO @ Sat, 11 Dec 2021 13:48:33: 4000000 INFO @ Sat, 11 Dec 2021 13:48:34: 11000000 INFO @ Sat, 11 Dec 2021 13:48:38: 5000000 INFO @ Sat, 11 Dec 2021 13:48:39: 12000000 INFO @ Sat, 11 Dec 2021 13:48:42: 6000000 INFO @ Sat, 11 Dec 2021 13:48:43: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:48:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:48:46: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:48:46: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:48:46: 7000000 INFO @ Sat, 11 Dec 2021 13:48:47: 14000000 INFO @ Sat, 11 Dec 2021 13:48:50: 1000000 INFO @ Sat, 11 Dec 2021 13:48:51: 8000000 INFO @ Sat, 11 Dec 2021 13:48:52: 15000000 INFO @ Sat, 11 Dec 2021 13:48:55: 2000000 INFO @ Sat, 11 Dec 2021 13:48:55: 9000000 INFO @ Sat, 11 Dec 2021 13:48:56: 16000000 INFO @ Sat, 11 Dec 2021 13:48:59: 3000000 INFO @ Sat, 11 Dec 2021 13:49:00: 10000000 INFO @ Sat, 11 Dec 2021 13:49:00: 17000000 INFO @ Sat, 11 Dec 2021 13:49:04: 4000000 INFO @ Sat, 11 Dec 2021 13:49:04: 11000000 INFO @ Sat, 11 Dec 2021 13:49:05: 18000000 INFO @ Sat, 11 Dec 2021 13:49:08: 5000000 INFO @ Sat, 11 Dec 2021 13:49:09: 12000000 INFO @ Sat, 11 Dec 2021 13:49:09: 19000000 INFO @ Sat, 11 Dec 2021 13:49:13: 6000000 INFO @ Sat, 11 Dec 2021 13:49:13: 13000000 INFO @ Sat, 11 Dec 2021 13:49:14: 20000000 INFO @ Sat, 11 Dec 2021 13:49:17: 7000000 INFO @ Sat, 11 Dec 2021 13:49:18: 14000000 INFO @ Sat, 11 Dec 2021 13:49:18: 21000000 INFO @ Sat, 11 Dec 2021 13:49:22: 8000000 INFO @ Sat, 11 Dec 2021 13:49:22: 15000000 INFO @ Sat, 11 Dec 2021 13:49:22: 22000000 INFO @ Sat, 11 Dec 2021 13:49:26: 9000000 INFO @ Sat, 11 Dec 2021 13:49:26: 16000000 INFO @ Sat, 11 Dec 2021 13:49:27: 23000000 INFO @ Sat, 11 Dec 2021 13:49:31: 17000000 INFO @ Sat, 11 Dec 2021 13:49:31: 10000000 INFO @ Sat, 11 Dec 2021 13:49:31: 24000000 INFO @ Sat, 11 Dec 2021 13:49:35: 18000000 INFO @ Sat, 11 Dec 2021 13:49:35: 11000000 INFO @ Sat, 11 Dec 2021 13:49:35: 25000000 INFO @ Sat, 11 Dec 2021 13:49:39: 19000000 INFO @ Sat, 11 Dec 2021 13:49:40: 12000000 INFO @ Sat, 11 Dec 2021 13:49:40: 26000000 INFO @ Sat, 11 Dec 2021 13:49:44: 20000000 INFO @ Sat, 11 Dec 2021 13:49:44: 13000000 INFO @ Sat, 11 Dec 2021 13:49:44: 27000000 INFO @ Sat, 11 Dec 2021 13:49:48: 21000000 INFO @ Sat, 11 Dec 2021 13:49:49: 14000000 INFO @ Sat, 11 Dec 2021 13:49:49: 28000000 INFO @ Sat, 11 Dec 2021 13:49:52: 22000000 INFO @ Sat, 11 Dec 2021 13:49:53: 29000000 INFO @ Sat, 11 Dec 2021 13:49:53: 15000000 INFO @ Sat, 11 Dec 2021 13:49:57: 23000000 INFO @ Sat, 11 Dec 2021 13:49:57: 30000000 INFO @ Sat, 11 Dec 2021 13:49:57: 16000000 INFO @ Sat, 11 Dec 2021 13:50:01: 24000000 INFO @ Sat, 11 Dec 2021 13:50:02: 17000000 INFO @ Sat, 11 Dec 2021 13:50:02: 31000000 INFO @ Sat, 11 Dec 2021 13:50:05: 25000000 INFO @ Sat, 11 Dec 2021 13:50:06: 18000000 INFO @ Sat, 11 Dec 2021 13:50:06: 32000000 INFO @ Sat, 11 Dec 2021 13:50:10: 26000000 INFO @ Sat, 11 Dec 2021 13:50:10: 19000000 INFO @ Sat, 11 Dec 2021 13:50:11: 33000000 INFO @ Sat, 11 Dec 2021 13:50:14: 27000000 INFO @ Sat, 11 Dec 2021 13:50:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:50:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:50:15: #1 total tags in treatment: 15603230 INFO @ Sat, 11 Dec 2021 13:50:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:50:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:50:15: 20000000 INFO @ Sat, 11 Dec 2021 13:50:15: #1 tags after filtering in treatment: 12768215 INFO @ Sat, 11 Dec 2021 13:50:15: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:50:15: #1 finished! INFO @ Sat, 11 Dec 2021 13:50:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:50:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:50:16: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:50:16: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:50:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:50:18: 28000000 INFO @ Sat, 11 Dec 2021 13:50:19: 21000000 INFO @ Sat, 11 Dec 2021 13:50:23: 29000000 INFO @ Sat, 11 Dec 2021 13:50:23: 22000000 INFO @ Sat, 11 Dec 2021 13:50:27: 30000000 INFO @ Sat, 11 Dec 2021 13:50:28: 23000000 INFO @ Sat, 11 Dec 2021 13:50:31: 31000000 INFO @ Sat, 11 Dec 2021 13:50:32: 24000000 INFO @ Sat, 11 Dec 2021 13:50:35: 32000000 INFO @ Sat, 11 Dec 2021 13:50:36: 25000000 INFO @ Sat, 11 Dec 2021 13:50:40: 33000000 INFO @ Sat, 11 Dec 2021 13:50:40: 26000000 INFO @ Sat, 11 Dec 2021 13:50:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:50:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:50:43: #1 total tags in treatment: 15603230 INFO @ Sat, 11 Dec 2021 13:50:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:50:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:50:44: #1 tags after filtering in treatment: 12768215 INFO @ Sat, 11 Dec 2021 13:50:44: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:50:44: #1 finished! INFO @ Sat, 11 Dec 2021 13:50:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:50:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:50:44: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:50:44: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:50:44: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 13:50:44: 27000000 cut: /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:50:49: 28000000 INFO @ Sat, 11 Dec 2021 13:50:53: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:50:57: 30000000 INFO @ Sat, 11 Dec 2021 13:51:01: 31000000 INFO @ Sat, 11 Dec 2021 13:51:05: 32000000 INFO @ Sat, 11 Dec 2021 13:51:09: 33000000 INFO @ Sat, 11 Dec 2021 13:51:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:51:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:51:13: #1 total tags in treatment: 15603230 INFO @ Sat, 11 Dec 2021 13:51:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:51:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:51:13: #1 tags after filtering in treatment: 12768215 INFO @ Sat, 11 Dec 2021 13:51:13: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:51:13: #1 finished! INFO @ Sat, 11 Dec 2021 13:51:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:51:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:51:14: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 13:51:14: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:51:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386246/SRX10386246.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。