Job ID = 14171774 SRX = SRX10386235 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 37158159 spots for SRR14009300/SRR14009300.sra Written 37158159 spots for SRR14009300/SRR14009300.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172553 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:27:34 37158159 reads; of these: 37158159 (100.00%) were paired; of these: 7178221 (19.32%) aligned concordantly 0 times 20668259 (55.62%) aligned concordantly exactly 1 time 9311679 (25.06%) aligned concordantly >1 times ---- 7178221 pairs aligned concordantly 0 times; of these: 167854 (2.34%) aligned discordantly 1 time ---- 7010367 pairs aligned 0 times concordantly or discordantly; of these: 14020734 mates make up the pairs; of these: 12677434 (90.42%) aligned 0 times 777114 (5.54%) aligned exactly 1 time 566186 (4.04%) aligned >1 times 82.94% overall alignment rate Time searching: 01:27:35 Overall time: 01:27:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 11486618 / 30094513 = 0.3817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:33:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:33:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:33:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:33:55: 1000000 INFO @ Sat, 11 Dec 2021 14:34:00: 2000000 INFO @ Sat, 11 Dec 2021 14:34:05: 3000000 INFO @ Sat, 11 Dec 2021 14:34:10: 4000000 INFO @ Sat, 11 Dec 2021 14:34:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:34:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:34:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:34:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:34:20: 6000000 INFO @ Sat, 11 Dec 2021 14:34:25: 1000000 INFO @ Sat, 11 Dec 2021 14:34:26: 7000000 INFO @ Sat, 11 Dec 2021 14:34:31: 8000000 INFO @ Sat, 11 Dec 2021 14:34:31: 2000000 INFO @ Sat, 11 Dec 2021 14:34:36: 9000000 INFO @ Sat, 11 Dec 2021 14:34:36: 3000000 INFO @ Sat, 11 Dec 2021 14:34:41: 10000000 INFO @ Sat, 11 Dec 2021 14:34:42: 4000000 INFO @ Sat, 11 Dec 2021 14:34:47: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:34:48: 5000000 INFO @ Sat, 11 Dec 2021 14:34:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:34:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:34:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:34:52: 12000000 INFO @ Sat, 11 Dec 2021 14:34:53: 6000000 INFO @ Sat, 11 Dec 2021 14:34:56: 1000000 INFO @ Sat, 11 Dec 2021 14:34:58: 13000000 INFO @ Sat, 11 Dec 2021 14:34:59: 7000000 INFO @ Sat, 11 Dec 2021 14:35:02: 2000000 INFO @ Sat, 11 Dec 2021 14:35:04: 14000000 INFO @ Sat, 11 Dec 2021 14:35:05: 8000000 INFO @ Sat, 11 Dec 2021 14:35:08: 3000000 INFO @ Sat, 11 Dec 2021 14:35:10: 15000000 INFO @ Sat, 11 Dec 2021 14:35:10: 9000000 INFO @ Sat, 11 Dec 2021 14:35:14: 4000000 INFO @ Sat, 11 Dec 2021 14:35:16: 10000000 INFO @ Sat, 11 Dec 2021 14:35:16: 16000000 INFO @ Sat, 11 Dec 2021 14:35:20: 5000000 INFO @ Sat, 11 Dec 2021 14:35:22: 11000000 INFO @ Sat, 11 Dec 2021 14:35:22: 17000000 INFO @ Sat, 11 Dec 2021 14:35:26: 6000000 INFO @ Sat, 11 Dec 2021 14:35:28: 12000000 INFO @ Sat, 11 Dec 2021 14:35:28: 18000000 INFO @ Sat, 11 Dec 2021 14:35:32: 7000000 INFO @ Sat, 11 Dec 2021 14:35:33: 13000000 INFO @ Sat, 11 Dec 2021 14:35:35: 19000000 INFO @ Sat, 11 Dec 2021 14:35:38: 8000000 INFO @ Sat, 11 Dec 2021 14:35:39: 14000000 INFO @ Sat, 11 Dec 2021 14:35:40: 20000000 INFO @ Sat, 11 Dec 2021 14:35:44: 9000000 INFO @ Sat, 11 Dec 2021 14:35:45: 15000000 INFO @ Sat, 11 Dec 2021 14:35:47: 21000000 INFO @ Sat, 11 Dec 2021 14:35:50: 10000000 INFO @ Sat, 11 Dec 2021 14:35:50: 16000000 INFO @ Sat, 11 Dec 2021 14:35:53: 22000000 INFO @ Sat, 11 Dec 2021 14:35:56: 11000000 INFO @ Sat, 11 Dec 2021 14:35:56: 17000000 INFO @ Sat, 11 Dec 2021 14:35:59: 23000000 INFO @ Sat, 11 Dec 2021 14:36:02: 12000000 INFO @ Sat, 11 Dec 2021 14:36:02: 18000000 INFO @ Sat, 11 Dec 2021 14:36:05: 24000000 INFO @ Sat, 11 Dec 2021 14:36:07: 19000000 INFO @ Sat, 11 Dec 2021 14:36:08: 13000000 INFO @ Sat, 11 Dec 2021 14:36:11: 25000000 INFO @ Sat, 11 Dec 2021 14:36:13: 20000000 INFO @ Sat, 11 Dec 2021 14:36:14: 14000000 INFO @ Sat, 11 Dec 2021 14:36:16: 26000000 INFO @ Sat, 11 Dec 2021 14:36:19: 21000000 INFO @ Sat, 11 Dec 2021 14:36:20: 15000000 INFO @ Sat, 11 Dec 2021 14:36:23: 27000000 INFO @ Sat, 11 Dec 2021 14:36:25: 22000000 INFO @ Sat, 11 Dec 2021 14:36:25: 16000000 INFO @ Sat, 11 Dec 2021 14:36:29: 28000000 INFO @ Sat, 11 Dec 2021 14:36:30: 23000000 INFO @ Sat, 11 Dec 2021 14:36:31: 17000000 INFO @ Sat, 11 Dec 2021 14:36:34: 29000000 INFO @ Sat, 11 Dec 2021 14:36:36: 24000000 INFO @ Sat, 11 Dec 2021 14:36:37: 18000000 INFO @ Sat, 11 Dec 2021 14:36:40: 30000000 INFO @ Sat, 11 Dec 2021 14:36:42: 25000000 INFO @ Sat, 11 Dec 2021 14:36:43: 19000000 INFO @ Sat, 11 Dec 2021 14:36:46: 31000000 INFO @ Sat, 11 Dec 2021 14:36:48: 26000000 INFO @ Sat, 11 Dec 2021 14:36:49: 20000000 INFO @ Sat, 11 Dec 2021 14:36:52: 32000000 INFO @ Sat, 11 Dec 2021 14:36:53: 27000000 INFO @ Sat, 11 Dec 2021 14:36:55: 21000000 INFO @ Sat, 11 Dec 2021 14:36:58: 33000000 INFO @ Sat, 11 Dec 2021 14:36:59: 28000000 INFO @ Sat, 11 Dec 2021 14:37:01: 22000000 INFO @ Sat, 11 Dec 2021 14:37:04: 34000000 INFO @ Sat, 11 Dec 2021 14:37:05: 29000000 INFO @ Sat, 11 Dec 2021 14:37:06: 23000000 INFO @ Sat, 11 Dec 2021 14:37:10: 35000000 INFO @ Sat, 11 Dec 2021 14:37:10: 30000000 INFO @ Sat, 11 Dec 2021 14:37:12: 24000000 INFO @ Sat, 11 Dec 2021 14:37:16: 36000000 INFO @ Sat, 11 Dec 2021 14:37:16: 31000000 INFO @ Sat, 11 Dec 2021 14:37:18: 25000000 INFO @ Sat, 11 Dec 2021 14:37:21: 37000000 INFO @ Sat, 11 Dec 2021 14:37:22: 32000000 INFO @ Sat, 11 Dec 2021 14:37:24: 26000000 INFO @ Sat, 11 Dec 2021 14:37:27: 38000000 INFO @ Sat, 11 Dec 2021 14:37:27: 33000000 INFO @ Sat, 11 Dec 2021 14:37:30: 27000000 INFO @ Sat, 11 Dec 2021 14:37:32: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:37:32: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:37:32: #1 total tags in treatment: 18519567 INFO @ Sat, 11 Dec 2021 14:37:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:37:32: #1 tags after filtering in treatment: 15331721 INFO @ Sat, 11 Dec 2021 14:37:32: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 14:37:32: #1 finished! INFO @ Sat, 11 Dec 2021 14:37:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:37:33: #2 number of paired peaks: 91 WARNING @ Sat, 11 Dec 2021 14:37:33: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:37:33: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 14:37:33: 34000000 cut: /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:37:36: 28000000 INFO @ Sat, 11 Dec 2021 14:37:39: 35000000 INFO @ Sat, 11 Dec 2021 14:37:42: 29000000 INFO @ Sat, 11 Dec 2021 14:37:45: 36000000 INFO @ Sat, 11 Dec 2021 14:37:48: 30000000 INFO @ Sat, 11 Dec 2021 14:37:50: 37000000 INFO @ Sat, 11 Dec 2021 14:37:54: 31000000 INFO @ Sat, 11 Dec 2021 14:37:56: 38000000 INFO @ Sat, 11 Dec 2021 14:38:00: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:38:00: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:38:00: #1 total tags in treatment: 18519567 INFO @ Sat, 11 Dec 2021 14:38:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:38:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:38:00: #1 tags after filtering in treatment: 15331721 INFO @ Sat, 11 Dec 2021 14:38:00: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 14:38:00: #1 finished! INFO @ Sat, 11 Dec 2021 14:38:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:38:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:38:01: 32000000 INFO @ Sat, 11 Dec 2021 14:38:01: #2 number of paired peaks: 91 WARNING @ Sat, 11 Dec 2021 14:38:01: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:38:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:38:07: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:38:13: 34000000 INFO @ Sat, 11 Dec 2021 14:38:18: 35000000 INFO @ Sat, 11 Dec 2021 14:38:24: 36000000 INFO @ Sat, 11 Dec 2021 14:38:30: 37000000 INFO @ Sat, 11 Dec 2021 14:38:37: 38000000 INFO @ Sat, 11 Dec 2021 14:38:42: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:38:42: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:38:42: #1 total tags in treatment: 18519567 INFO @ Sat, 11 Dec 2021 14:38:42: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:38:42: #1 tags after filtering in treatment: 15331721 INFO @ Sat, 11 Dec 2021 14:38:42: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 14:38:42: #1 finished! INFO @ Sat, 11 Dec 2021 14:38:42: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:38:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:38:43: #2 number of paired peaks: 91 WARNING @ Sat, 11 Dec 2021 14:38:43: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:38:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386235/SRX10386235.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。