Job ID = 14171722 SRX = SRX10386224 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 23353114 spots for SRR14009289/SRR14009289.sra Written 23353114 spots for SRR14009289/SRR14009289.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172305 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:14 23353114 reads; of these: 23353114 (100.00%) were paired; of these: 3203544 (13.72%) aligned concordantly 0 times 14935938 (63.96%) aligned concordantly exactly 1 time 5213632 (22.33%) aligned concordantly >1 times ---- 3203544 pairs aligned concordantly 0 times; of these: 463192 (14.46%) aligned discordantly 1 time ---- 2740352 pairs aligned 0 times concordantly or discordantly; of these: 5480704 mates make up the pairs; of these: 4271144 (77.93%) aligned 0 times 597908 (10.91%) aligned exactly 1 time 611652 (11.16%) aligned >1 times 90.86% overall alignment rate Time searching: 00:37:14 Overall time: 00:37:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2186811 / 20583868 = 0.1062 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:25:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:25:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:25:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:25:17: 1000000 INFO @ Sat, 11 Dec 2021 13:25:22: 2000000 INFO @ Sat, 11 Dec 2021 13:25:28: 3000000 INFO @ Sat, 11 Dec 2021 13:25:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:25:39: 5000000 INFO @ Sat, 11 Dec 2021 13:25:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:25:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:25:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:25:45: 6000000 INFO @ Sat, 11 Dec 2021 13:25:47: 1000000 INFO @ Sat, 11 Dec 2021 13:25:51: 7000000 INFO @ Sat, 11 Dec 2021 13:25:53: 2000000 INFO @ Sat, 11 Dec 2021 13:25:58: 8000000 INFO @ Sat, 11 Dec 2021 13:25:59: 3000000 INFO @ Sat, 11 Dec 2021 13:26:04: 9000000 INFO @ Sat, 11 Dec 2021 13:26:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:26:10: 10000000 INFO @ Sat, 11 Dec 2021 13:26:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:26:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:26:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:26:11: 5000000 INFO @ Sat, 11 Dec 2021 13:26:15: 11000000 INFO @ Sat, 11 Dec 2021 13:26:16: 1000000 INFO @ Sat, 11 Dec 2021 13:26:17: 6000000 INFO @ Sat, 11 Dec 2021 13:26:21: 12000000 INFO @ Sat, 11 Dec 2021 13:26:21: 2000000 INFO @ Sat, 11 Dec 2021 13:26:23: 7000000 INFO @ Sat, 11 Dec 2021 13:26:27: 3000000 INFO @ Sat, 11 Dec 2021 13:26:27: 13000000 INFO @ Sat, 11 Dec 2021 13:26:29: 8000000 INFO @ Sat, 11 Dec 2021 13:26:32: 4000000 INFO @ Sat, 11 Dec 2021 13:26:33: 14000000 INFO @ Sat, 11 Dec 2021 13:26:36: 9000000 INFO @ Sat, 11 Dec 2021 13:26:37: 5000000 INFO @ Sat, 11 Dec 2021 13:26:39: 15000000 INFO @ Sat, 11 Dec 2021 13:26:42: 10000000 INFO @ Sat, 11 Dec 2021 13:26:43: 6000000 INFO @ Sat, 11 Dec 2021 13:26:45: 16000000 INFO @ Sat, 11 Dec 2021 13:26:48: 7000000 INFO @ Sat, 11 Dec 2021 13:26:48: 11000000 INFO @ Sat, 11 Dec 2021 13:26:51: 17000000 INFO @ Sat, 11 Dec 2021 13:26:53: 8000000 INFO @ Sat, 11 Dec 2021 13:26:54: 12000000 INFO @ Sat, 11 Dec 2021 13:26:56: 18000000 INFO @ Sat, 11 Dec 2021 13:26:58: 9000000 INFO @ Sat, 11 Dec 2021 13:27:00: 13000000 INFO @ Sat, 11 Dec 2021 13:27:02: 19000000 INFO @ Sat, 11 Dec 2021 13:27:04: 10000000 INFO @ Sat, 11 Dec 2021 13:27:07: 14000000 INFO @ Sat, 11 Dec 2021 13:27:08: 20000000 INFO @ Sat, 11 Dec 2021 13:27:09: 11000000 INFO @ Sat, 11 Dec 2021 13:27:13: 15000000 INFO @ Sat, 11 Dec 2021 13:27:14: 21000000 INFO @ Sat, 11 Dec 2021 13:27:14: 12000000 INFO @ Sat, 11 Dec 2021 13:27:19: 16000000 INFO @ Sat, 11 Dec 2021 13:27:19: 22000000 INFO @ Sat, 11 Dec 2021 13:27:20: 13000000 INFO @ Sat, 11 Dec 2021 13:27:25: 14000000 INFO @ Sat, 11 Dec 2021 13:27:25: 17000000 INFO @ Sat, 11 Dec 2021 13:27:25: 23000000 INFO @ Sat, 11 Dec 2021 13:27:30: 15000000 INFO @ Sat, 11 Dec 2021 13:27:31: 18000000 INFO @ Sat, 11 Dec 2021 13:27:31: 24000000 INFO @ Sat, 11 Dec 2021 13:27:35: 16000000 INFO @ Sat, 11 Dec 2021 13:27:37: 25000000 INFO @ Sat, 11 Dec 2021 13:27:37: 19000000 INFO @ Sat, 11 Dec 2021 13:27:41: 17000000 INFO @ Sat, 11 Dec 2021 13:27:43: 26000000 INFO @ Sat, 11 Dec 2021 13:27:43: 20000000 INFO @ Sat, 11 Dec 2021 13:27:46: 18000000 INFO @ Sat, 11 Dec 2021 13:27:49: 27000000 INFO @ Sat, 11 Dec 2021 13:27:49: 21000000 INFO @ Sat, 11 Dec 2021 13:27:51: 19000000 INFO @ Sat, 11 Dec 2021 13:27:55: 28000000 INFO @ Sat, 11 Dec 2021 13:27:55: 22000000 INFO @ Sat, 11 Dec 2021 13:27:57: 20000000 INFO @ Sat, 11 Dec 2021 13:28:01: 23000000 INFO @ Sat, 11 Dec 2021 13:28:01: 29000000 INFO @ Sat, 11 Dec 2021 13:28:02: 21000000 INFO @ Sat, 11 Dec 2021 13:28:07: 22000000 INFO @ Sat, 11 Dec 2021 13:28:07: 24000000 INFO @ Sat, 11 Dec 2021 13:28:07: 30000000 INFO @ Sat, 11 Dec 2021 13:28:12: 23000000 INFO @ Sat, 11 Dec 2021 13:28:13: 25000000 INFO @ Sat, 11 Dec 2021 13:28:14: 31000000 INFO @ Sat, 11 Dec 2021 13:28:18: 24000000 INFO @ Sat, 11 Dec 2021 13:28:20: 26000000 INFO @ Sat, 11 Dec 2021 13:28:20: 32000000 INFO @ Sat, 11 Dec 2021 13:28:23: 25000000 INFO @ Sat, 11 Dec 2021 13:28:26: 27000000 INFO @ Sat, 11 Dec 2021 13:28:26: 33000000 INFO @ Sat, 11 Dec 2021 13:28:28: 26000000 INFO @ Sat, 11 Dec 2021 13:28:32: 28000000 INFO @ Sat, 11 Dec 2021 13:28:32: 34000000 INFO @ Sat, 11 Dec 2021 13:28:33: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:28:38: 29000000 INFO @ Sat, 11 Dec 2021 13:28:38: 35000000 INFO @ Sat, 11 Dec 2021 13:28:39: 28000000 INFO @ Sat, 11 Dec 2021 13:28:44: 29000000 INFO @ Sat, 11 Dec 2021 13:28:45: 30000000 INFO @ Sat, 11 Dec 2021 13:28:45: 36000000 INFO @ Sat, 11 Dec 2021 13:28:49: 30000000 INFO @ Sat, 11 Dec 2021 13:28:51: 31000000 INFO @ Sat, 11 Dec 2021 13:28:51: 37000000 INFO @ Sat, 11 Dec 2021 13:28:55: 31000000 INFO @ Sat, 11 Dec 2021 13:28:57: 32000000 INFO @ Sat, 11 Dec 2021 13:28:57: 38000000 INFO @ Sat, 11 Dec 2021 13:28:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:28:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:28:58: #1 total tags in treatment: 17977692 INFO @ Sat, 11 Dec 2021 13:28:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:28:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:28:58: #1 tags after filtering in treatment: 14789441 INFO @ Sat, 11 Dec 2021 13:28:58: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:28:58: #1 finished! INFO @ Sat, 11 Dec 2021 13:28:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:28:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:28:59: #2 number of paired peaks: 65 WARNING @ Sat, 11 Dec 2021 13:28:59: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:28:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:29:00: 32000000 INFO @ Sat, 11 Dec 2021 13:29:03: 33000000 INFO @ Sat, 11 Dec 2021 13:29:05: 33000000 INFO @ Sat, 11 Dec 2021 13:29:09: 34000000 INFO @ Sat, 11 Dec 2021 13:29:11: 34000000 INFO @ Sat, 11 Dec 2021 13:29:15: 35000000 INFO @ Sat, 11 Dec 2021 13:29:17: 35000000 INFO @ Sat, 11 Dec 2021 13:29:22: 36000000 INFO @ Sat, 11 Dec 2021 13:29:22: 36000000 INFO @ Sat, 11 Dec 2021 13:29:28: 37000000 INFO @ Sat, 11 Dec 2021 13:29:28: 37000000 INFO @ Sat, 11 Dec 2021 13:29:34: 38000000 INFO @ Sat, 11 Dec 2021 13:29:34: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:29:34: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:29:34: #1 total tags in treatment: 17977692 INFO @ Sat, 11 Dec 2021 13:29:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:29:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:29:35: 38000000 INFO @ Sat, 11 Dec 2021 13:29:35: #1 tags after filtering in treatment: 14789441 INFO @ Sat, 11 Dec 2021 13:29:35: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:29:35: #1 finished! INFO @ Sat, 11 Dec 2021 13:29:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:29:35: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:29:35: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:29:35: #1 total tags in treatment: 17977692 INFO @ Sat, 11 Dec 2021 13:29:35: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:29:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:29:35: #1 tags after filtering in treatment: 14789441 INFO @ Sat, 11 Dec 2021 13:29:35: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 11 Dec 2021 13:29:35: #1 finished! INFO @ Sat, 11 Dec 2021 13:29:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:29:36: #2 number of paired peaks: 65 WARNING @ Sat, 11 Dec 2021 13:29:36: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:29:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:29:36: #2 number of paired peaks: 65 WARNING @ Sat, 11 Dec 2021 13:29:36: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:29:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386224/SRX10386224.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。