Job ID = 14171645 SRX = SRX10386214 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28797700 spots for SRR14009279/SRR14009279.sra Written 28797700 spots for SRR14009279/SRR14009279.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172231 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:36 28797700 reads; of these: 28797700 (100.00%) were paired; of these: 6375262 (22.14%) aligned concordantly 0 times 16588920 (57.61%) aligned concordantly exactly 1 time 5833518 (20.26%) aligned concordantly >1 times ---- 6375262 pairs aligned concordantly 0 times; of these: 53699 (0.84%) aligned discordantly 1 time ---- 6321563 pairs aligned 0 times concordantly or discordantly; of these: 12643126 mates make up the pairs; of these: 11707512 (92.60%) aligned 0 times 582014 (4.60%) aligned exactly 1 time 353600 (2.80%) aligned >1 times 79.67% overall alignment rate Time searching: 00:41:36 Overall time: 00:41:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5147447 / 22461582 = 0.2292 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:07:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:07:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:07:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:07:56: 1000000 INFO @ Sat, 11 Dec 2021 13:08:01: 2000000 INFO @ Sat, 11 Dec 2021 13:08:06: 3000000 INFO @ Sat, 11 Dec 2021 13:08:11: 4000000 INFO @ Sat, 11 Dec 2021 13:08:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:08:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:08:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:08:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:08:21: 6000000 INFO @ Sat, 11 Dec 2021 13:08:27: 7000000 INFO @ Sat, 11 Dec 2021 13:08:28: 1000000 INFO @ Sat, 11 Dec 2021 13:08:33: 8000000 INFO @ Sat, 11 Dec 2021 13:08:35: 2000000 INFO @ Sat, 11 Dec 2021 13:08:39: 9000000 INFO @ Sat, 11 Dec 2021 13:08:41: 3000000 INFO @ Sat, 11 Dec 2021 13:08:45: 10000000 INFO @ Sat, 11 Dec 2021 13:08:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:08:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:08:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:08:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:08:51: 11000000 INFO @ Sat, 11 Dec 2021 13:08:55: 5000000 INFO @ Sat, 11 Dec 2021 13:08:57: 1000000 INFO @ Sat, 11 Dec 2021 13:08:58: 12000000 INFO @ Sat, 11 Dec 2021 13:09:02: 6000000 INFO @ Sat, 11 Dec 2021 13:09:03: 2000000 INFO @ Sat, 11 Dec 2021 13:09:04: 13000000 INFO @ Sat, 11 Dec 2021 13:09:09: 7000000 INFO @ Sat, 11 Dec 2021 13:09:09: 3000000 INFO @ Sat, 11 Dec 2021 13:09:10: 14000000 INFO @ Sat, 11 Dec 2021 13:09:15: 4000000 INFO @ Sat, 11 Dec 2021 13:09:16: 8000000 INFO @ Sat, 11 Dec 2021 13:09:16: 15000000 INFO @ Sat, 11 Dec 2021 13:09:21: 5000000 INFO @ Sat, 11 Dec 2021 13:09:22: 16000000 INFO @ Sat, 11 Dec 2021 13:09:22: 9000000 INFO @ Sat, 11 Dec 2021 13:09:27: 6000000 INFO @ Sat, 11 Dec 2021 13:09:28: 17000000 INFO @ Sat, 11 Dec 2021 13:09:29: 10000000 INFO @ Sat, 11 Dec 2021 13:09:33: 7000000 INFO @ Sat, 11 Dec 2021 13:09:34: 18000000 INFO @ Sat, 11 Dec 2021 13:09:36: 11000000 INFO @ Sat, 11 Dec 2021 13:09:39: 8000000 INFO @ Sat, 11 Dec 2021 13:09:40: 19000000 INFO @ Sat, 11 Dec 2021 13:09:43: 12000000 INFO @ Sat, 11 Dec 2021 13:09:45: 9000000 INFO @ Sat, 11 Dec 2021 13:09:46: 20000000 INFO @ Sat, 11 Dec 2021 13:09:50: 13000000 INFO @ Sat, 11 Dec 2021 13:09:51: 10000000 INFO @ Sat, 11 Dec 2021 13:09:52: 21000000 INFO @ Sat, 11 Dec 2021 13:09:56: 14000000 INFO @ Sat, 11 Dec 2021 13:09:57: 11000000 INFO @ Sat, 11 Dec 2021 13:09:58: 22000000 INFO @ Sat, 11 Dec 2021 13:10:03: 15000000 INFO @ Sat, 11 Dec 2021 13:10:03: 12000000 INFO @ Sat, 11 Dec 2021 13:10:04: 23000000 INFO @ Sat, 11 Dec 2021 13:10:09: 13000000 INFO @ Sat, 11 Dec 2021 13:10:10: 16000000 INFO @ Sat, 11 Dec 2021 13:10:11: 24000000 INFO @ Sat, 11 Dec 2021 13:10:15: 14000000 INFO @ Sat, 11 Dec 2021 13:10:17: 17000000 INFO @ Sat, 11 Dec 2021 13:10:17: 25000000 INFO @ Sat, 11 Dec 2021 13:10:21: 15000000 INFO @ Sat, 11 Dec 2021 13:10:23: 26000000 INFO @ Sat, 11 Dec 2021 13:10:23: 18000000 INFO @ Sat, 11 Dec 2021 13:10:27: 16000000 INFO @ Sat, 11 Dec 2021 13:10:29: 27000000 INFO @ Sat, 11 Dec 2021 13:10:30: 19000000 INFO @ Sat, 11 Dec 2021 13:10:33: 17000000 INFO @ Sat, 11 Dec 2021 13:10:35: 28000000 INFO @ Sat, 11 Dec 2021 13:10:36: 20000000 INFO @ Sat, 11 Dec 2021 13:10:39: 18000000 INFO @ Sat, 11 Dec 2021 13:10:41: 29000000 INFO @ Sat, 11 Dec 2021 13:10:43: 21000000 INFO @ Sat, 11 Dec 2021 13:10:45: 19000000 INFO @ Sat, 11 Dec 2021 13:10:47: 30000000 INFO @ Sat, 11 Dec 2021 13:10:50: 22000000 INFO @ Sat, 11 Dec 2021 13:10:51: 20000000 INFO @ Sat, 11 Dec 2021 13:10:54: 31000000 INFO @ Sat, 11 Dec 2021 13:10:57: 23000000 INFO @ Sat, 11 Dec 2021 13:10:57: 21000000 INFO @ Sat, 11 Dec 2021 13:11:00: 32000000 INFO @ Sat, 11 Dec 2021 13:11:03: 22000000 INFO @ Sat, 11 Dec 2021 13:11:04: 24000000 INFO @ Sat, 11 Dec 2021 13:11:06: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:11:09: 23000000 INFO @ Sat, 11 Dec 2021 13:11:10: 25000000 INFO @ Sat, 11 Dec 2021 13:11:12: 34000000 INFO @ Sat, 11 Dec 2021 13:11:15: 24000000 INFO @ Sat, 11 Dec 2021 13:11:17: 26000000 INFO @ Sat, 11 Dec 2021 13:11:18: 35000000 INFO @ Sat, 11 Dec 2021 13:11:21: 25000000 INFO @ Sat, 11 Dec 2021 13:11:22: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:11:22: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:11:22: #1 total tags in treatment: 17278373 INFO @ Sat, 11 Dec 2021 13:11:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:11:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:11:22: #1 tags after filtering in treatment: 14884952 INFO @ Sat, 11 Dec 2021 13:11:22: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 11 Dec 2021 13:11:22: #1 finished! INFO @ Sat, 11 Dec 2021 13:11:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:11:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:11:23: #2 number of paired peaks: 45 WARNING @ Sat, 11 Dec 2021 13:11:23: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:11:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:11:23: 27000000 INFO @ Sat, 11 Dec 2021 13:11:27: 26000000 INFO @ Sat, 11 Dec 2021 13:11:30: 28000000 INFO @ Sat, 11 Dec 2021 13:11:33: 27000000 INFO @ Sat, 11 Dec 2021 13:11:37: 29000000 INFO @ Sat, 11 Dec 2021 13:11:39: 28000000 INFO @ Sat, 11 Dec 2021 13:11:43: 30000000 INFO @ Sat, 11 Dec 2021 13:11:45: 29000000 INFO @ Sat, 11 Dec 2021 13:11:50: 31000000 INFO @ Sat, 11 Dec 2021 13:11:51: 30000000 INFO @ Sat, 11 Dec 2021 13:11:57: 32000000 INFO @ Sat, 11 Dec 2021 13:11:57: 31000000 INFO @ Sat, 11 Dec 2021 13:12:03: 32000000 INFO @ Sat, 11 Dec 2021 13:12:04: 33000000 INFO @ Sat, 11 Dec 2021 13:12:09: 33000000 INFO @ Sat, 11 Dec 2021 13:12:10: 34000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:12:15: 34000000 INFO @ Sat, 11 Dec 2021 13:12:17: 35000000 INFO @ Sat, 11 Dec 2021 13:12:21: 35000000 INFO @ Sat, 11 Dec 2021 13:12:21: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:12:21: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:12:21: #1 total tags in treatment: 17278373 INFO @ Sat, 11 Dec 2021 13:12:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:12:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:12:21: #1 tags after filtering in treatment: 14884952 INFO @ Sat, 11 Dec 2021 13:12:21: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 11 Dec 2021 13:12:21: #1 finished! INFO @ Sat, 11 Dec 2021 13:12:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:12:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:12:22: #2 number of paired peaks: 45 WARNING @ Sat, 11 Dec 2021 13:12:22: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:12:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:12:25: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:12:25: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:12:25: #1 total tags in treatment: 17278373 INFO @ Sat, 11 Dec 2021 13:12:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:12:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:12:25: #1 tags after filtering in treatment: 14884952 INFO @ Sat, 11 Dec 2021 13:12:25: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 11 Dec 2021 13:12:25: #1 finished! INFO @ Sat, 11 Dec 2021 13:12:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:12:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:12:26: #2 number of paired peaks: 45 WARNING @ Sat, 11 Dec 2021 13:12:26: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:12:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386214/SRX10386214.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling