Job ID = 14167171 SRX = SRX10340471 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 340478 READS because READLEN < 1 Read 2748849 spots for SRR13962480/SRR13962480.sra Written 2748849 spots for SRR13962480/SRR13962480.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167665 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] 3 unmatched pairs [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] 3 unmatched pairs [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] 4 unmatched pairs [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] 5 unmatched pairs [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 351673 / 2043582 = 0.1721 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:49:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:49:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:49:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:49:37: 1000000 INFO @ Fri, 10 Dec 2021 10:49:44: 2000000 INFO @ Fri, 10 Dec 2021 10:49:51: 3000000 INFO @ Fri, 10 Dec 2021 10:49:56: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:49:56: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:49:56: #1 total tags in treatment: 1576011 INFO @ Fri, 10 Dec 2021 10:49:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:49:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:49:56: #1 tags after filtering in treatment: 1552048 INFO @ Fri, 10 Dec 2021 10:49:56: #1 Redundant rate of treatment: 0.02 INFO @ Fri, 10 Dec 2021 10:49:56: #1 finished! INFO @ Fri, 10 Dec 2021 10:49:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:49:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:49:56: #2 number of paired peaks: 98 WARNING @ Fri, 10 Dec 2021 10:49:56: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:50:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:50:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:50:07: 1000000 INFO @ Fri, 10 Dec 2021 10:50:14: 2000000 INFO @ Fri, 10 Dec 2021 10:50:21: 3000000 INFO @ Fri, 10 Dec 2021 10:50:26: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:50:26: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:50:26: #1 total tags in treatment: 1576011 INFO @ Fri, 10 Dec 2021 10:50:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:50:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:50:26: #1 tags after filtering in treatment: 1552048 INFO @ Fri, 10 Dec 2021 10:50:26: #1 Redundant rate of treatment: 0.02 INFO @ Fri, 10 Dec 2021 10:50:26: #1 finished! INFO @ Fri, 10 Dec 2021 10:50:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:50:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:50:27: #2 number of paired peaks: 98 WARNING @ Fri, 10 Dec 2021 10:50:27: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:50:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:50:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:50:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:50:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:50:37: 1000000 INFO @ Fri, 10 Dec 2021 10:50:44: 2000000 INFO @ Fri, 10 Dec 2021 10:50:51: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:50:56: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:50:56: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:50:56: #1 total tags in treatment: 1576011 INFO @ Fri, 10 Dec 2021 10:50:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:50:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:50:56: #1 tags after filtering in treatment: 1552048 INFO @ Fri, 10 Dec 2021 10:50:56: #1 Redundant rate of treatment: 0.02 INFO @ Fri, 10 Dec 2021 10:50:56: #1 finished! INFO @ Fri, 10 Dec 2021 10:50:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:50:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:50:57: #2 number of paired peaks: 98 WARNING @ Fri, 10 Dec 2021 10:50:57: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:50:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10340471/SRX10340471.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。