Job ID = 14167129 SRX = SRX10340448 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 1029708 READS because READLEN < 1 Read 7578628 spots for SRR13962457/SRR13962457.sra Written 7578628 spots for SRR13962457/SRR13962457.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167625 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] 4 unmatched pairs [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] 5 unmatched pairs [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] 2 unmatched pairs [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] 5 unmatched pairs [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] 6 unmatched pairs [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2961367 / 5007562 = 0.5914 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:42:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:42:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:42:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:42:31: 1000000 INFO @ Fri, 10 Dec 2021 10:42:41: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:42:50: 3000000 INFO @ Fri, 10 Dec 2021 10:42:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:42:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:42:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:43:01: 1000000 INFO @ Fri, 10 Dec 2021 10:43:01: 4000000 INFO @ Fri, 10 Dec 2021 10:43:10: 2000000 INFO @ Fri, 10 Dec 2021 10:43:11: 5000000 INFO @ Fri, 10 Dec 2021 10:43:11: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:43:11: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:43:11: #1 total tags in treatment: 1872578 INFO @ Fri, 10 Dec 2021 10:43:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:43:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:43:11: #1 tags after filtering in treatment: 1817589 INFO @ Fri, 10 Dec 2021 10:43:11: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 10:43:11: #1 finished! INFO @ Fri, 10 Dec 2021 10:43:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:43:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:43:11: #2 number of paired peaks: 2059 INFO @ Fri, 10 Dec 2021 10:43:11: start model_add_line... INFO @ Fri, 10 Dec 2021 10:43:11: start X-correlation... INFO @ Fri, 10 Dec 2021 10:43:11: end of X-cor INFO @ Fri, 10 Dec 2021 10:43:11: #2 finished! INFO @ Fri, 10 Dec 2021 10:43:11: #2 predicted fragment length is 249 bps INFO @ Fri, 10 Dec 2021 10:43:11: #2 alternative fragment length(s) may be 249 bps INFO @ Fri, 10 Dec 2021 10:43:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05_model.r WARNING @ Fri, 10 Dec 2021 10:43:12: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 10:43:12: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Fri, 10 Dec 2021 10:43:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 10:43:12: #3 Call peaks... INFO @ Fri, 10 Dec 2021 10:43:12: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... INFO @ Fri, 10 Dec 2021 10:43:18: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:43:20: 3000000 INFO @ Fri, 10 Dec 2021 10:43:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:43:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:43:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:43:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05_peaks.xls INFO @ Fri, 10 Dec 2021 10:43:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 10:43:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.05_summits.bed INFO @ Fri, 10 Dec 2021 10:43:22: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2819 records, 4 fields): 32 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:43:30: 4000000 INFO @ Fri, 10 Dec 2021 10:43:31: 1000000 INFO @ Fri, 10 Dec 2021 10:43:40: 5000000 INFO @ Fri, 10 Dec 2021 10:43:40: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:43:40: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:43:40: #1 total tags in treatment: 1872578 INFO @ Fri, 10 Dec 2021 10:43:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:43:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:43:40: #1 tags after filtering in treatment: 1817589 INFO @ Fri, 10 Dec 2021 10:43:40: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 10:43:40: #1 finished! INFO @ Fri, 10 Dec 2021 10:43:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:43:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:43:41: #2 number of paired peaks: 2059 INFO @ Fri, 10 Dec 2021 10:43:41: start model_add_line... INFO @ Fri, 10 Dec 2021 10:43:41: start X-correlation... INFO @ Fri, 10 Dec 2021 10:43:41: end of X-cor INFO @ Fri, 10 Dec 2021 10:43:41: #2 finished! INFO @ Fri, 10 Dec 2021 10:43:41: #2 predicted fragment length is 249 bps INFO @ Fri, 10 Dec 2021 10:43:41: #2 alternative fragment length(s) may be 249 bps INFO @ Fri, 10 Dec 2021 10:43:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10_model.r WARNING @ Fri, 10 Dec 2021 10:43:41: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 10:43:41: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Fri, 10 Dec 2021 10:43:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 10:43:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 10:43:41: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 10:43:41: 2000000 INFO @ Fri, 10 Dec 2021 10:43:47: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 10:43:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10_peaks.xls INFO @ Fri, 10 Dec 2021 10:43:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 10:43:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.10_summits.bed INFO @ Fri, 10 Dec 2021 10:43:50: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (1894 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:43:51: 3000000 INFO @ Fri, 10 Dec 2021 10:44:01: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:44:11: 5000000 INFO @ Fri, 10 Dec 2021 10:44:11: #1 tag size is determined as 125 bps INFO @ Fri, 10 Dec 2021 10:44:11: #1 tag size = 125 INFO @ Fri, 10 Dec 2021 10:44:11: #1 total tags in treatment: 1872578 INFO @ Fri, 10 Dec 2021 10:44:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:44:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:44:11: #1 tags after filtering in treatment: 1817589 INFO @ Fri, 10 Dec 2021 10:44:11: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 10:44:11: #1 finished! INFO @ Fri, 10 Dec 2021 10:44:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:44:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:44:11: #2 number of paired peaks: 2059 INFO @ Fri, 10 Dec 2021 10:44:11: start model_add_line... INFO @ Fri, 10 Dec 2021 10:44:11: start X-correlation... INFO @ Fri, 10 Dec 2021 10:44:11: end of X-cor INFO @ Fri, 10 Dec 2021 10:44:11: #2 finished! INFO @ Fri, 10 Dec 2021 10:44:11: #2 predicted fragment length is 249 bps INFO @ Fri, 10 Dec 2021 10:44:11: #2 alternative fragment length(s) may be 249 bps INFO @ Fri, 10 Dec 2021 10:44:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20_model.r WARNING @ Fri, 10 Dec 2021 10:44:11: #2 Since the d (249) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 10:44:11: #2 You may need to consider one of the other alternative d(s): 249 WARNING @ Fri, 10 Dec 2021 10:44:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 10:44:11: #3 Call peaks... INFO @ Fri, 10 Dec 2021 10:44:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 10:44:17: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 10:44:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20_peaks.xls INFO @ Fri, 10 Dec 2021 10:44:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 10:44:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10340448/SRX10340448.20_summits.bed INFO @ Fri, 10 Dec 2021 10:44:21: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (834 records, 4 fields): 4 millis CompletedMACS2peakCalling BigWig に変換しました。