Job ID = 9158125 sra ファイルのダウンロード中... Completed: 1284330K bytes transferred in 10 seconds (989079K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 57619412 spots for /home/okishinya/chipatlas/results/dm3/SRX101815/SRR360701.sra Written 57619412 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:34 57619412 reads; of these: 57619412 (100.00%) were unpaired; of these: 14063406 (24.41%) aligned 0 times 28416013 (49.32%) aligned exactly 1 time 15139993 (26.28%) aligned >1 times 75.59% overall alignment rate Time searching: 00:17:35 Overall time: 00:17:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8967309 / 43556006 = 0.2059 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:40:46: # Command line: callpeak -t SRX101815.bam -f BAM -g dm -n SRX101815.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX101815.05 # format = BAM # ChIP-seq file = ['SRX101815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:40:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:40:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:40:46: # Command line: callpeak -t SRX101815.bam -f BAM -g dm -n SRX101815.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX101815.10 # format = BAM # ChIP-seq file = ['SRX101815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:40:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:40:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:40:46: # Command line: callpeak -t SRX101815.bam -f BAM -g dm -n SRX101815.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX101815.20 # format = BAM # ChIP-seq file = ['SRX101815.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:40:46: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:40:46: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:40:52: 1000000 INFO @ Tue, 27 Jun 2017 15:40:53: 1000000 INFO @ Tue, 27 Jun 2017 15:40:53: 1000000 INFO @ Tue, 27 Jun 2017 15:40:58: 2000000 INFO @ Tue, 27 Jun 2017 15:41:00: 2000000 INFO @ Tue, 27 Jun 2017 15:41:01: 2000000 INFO @ Tue, 27 Jun 2017 15:41:04: 3000000 INFO @ Tue, 27 Jun 2017 15:41:07: 3000000 INFO @ Tue, 27 Jun 2017 15:41:09: 3000000 INFO @ Tue, 27 Jun 2017 15:41:11: 4000000 INFO @ Tue, 27 Jun 2017 15:41:14: 4000000 INFO @ Tue, 27 Jun 2017 15:41:16: 4000000 INFO @ Tue, 27 Jun 2017 15:41:17: 5000000 INFO @ Tue, 27 Jun 2017 15:41:21: 5000000 INFO @ Tue, 27 Jun 2017 15:41:23: 6000000 INFO @ Tue, 27 Jun 2017 15:41:24: 5000000 INFO @ Tue, 27 Jun 2017 15:41:28: 6000000 INFO @ Tue, 27 Jun 2017 15:41:29: 7000000 INFO @ Tue, 27 Jun 2017 15:41:32: 6000000 INFO @ Tue, 27 Jun 2017 15:41:35: 8000000 INFO @ Tue, 27 Jun 2017 15:41:36: 7000000 INFO @ Tue, 27 Jun 2017 15:41:40: 7000000 INFO @ Tue, 27 Jun 2017 15:41:41: 9000000 INFO @ Tue, 27 Jun 2017 15:41:43: 8000000 INFO @ Tue, 27 Jun 2017 15:41:47: 8000000 INFO @ Tue, 27 Jun 2017 15:41:48: 10000000 INFO @ Tue, 27 Jun 2017 15:41:50: 9000000 INFO @ Tue, 27 Jun 2017 15:41:54: 11000000 INFO @ Tue, 27 Jun 2017 15:41:55: 9000000 INFO @ Tue, 27 Jun 2017 15:41:57: 10000000 INFO @ Tue, 27 Jun 2017 15:42:00: 12000000 INFO @ Tue, 27 Jun 2017 15:42:03: 10000000 INFO @ Tue, 27 Jun 2017 15:42:04: 11000000 INFO @ Tue, 27 Jun 2017 15:42:07: 13000000 INFO @ Tue, 27 Jun 2017 15:42:10: 11000000 INFO @ Tue, 27 Jun 2017 15:42:11: 12000000 INFO @ Tue, 27 Jun 2017 15:42:13: 14000000 INFO @ Tue, 27 Jun 2017 15:42:18: 12000000 INFO @ Tue, 27 Jun 2017 15:42:18: 13000000 INFO @ Tue, 27 Jun 2017 15:42:19: 15000000 INFO @ Tue, 27 Jun 2017 15:42:25: 14000000 INFO @ Tue, 27 Jun 2017 15:42:25: 13000000 INFO @ Tue, 27 Jun 2017 15:42:25: 16000000 INFO @ Tue, 27 Jun 2017 15:42:32: 17000000 INFO @ Tue, 27 Jun 2017 15:42:32: 15000000 INFO @ Tue, 27 Jun 2017 15:42:33: 14000000 INFO @ Tue, 27 Jun 2017 15:42:38: 18000000 INFO @ Tue, 27 Jun 2017 15:42:39: 16000000 INFO @ Tue, 27 Jun 2017 15:42:40: 15000000 INFO @ Tue, 27 Jun 2017 15:42:44: 19000000 INFO @ Tue, 27 Jun 2017 15:42:46: 17000000 INFO @ Tue, 27 Jun 2017 15:42:48: 16000000 INFO @ Tue, 27 Jun 2017 15:42:50: 20000000 INFO @ Tue, 27 Jun 2017 15:42:53: 18000000 INFO @ Tue, 27 Jun 2017 15:42:56: 17000000 INFO @ Tue, 27 Jun 2017 15:42:57: 21000000 INFO @ Tue, 27 Jun 2017 15:43:00: 19000000 INFO @ Tue, 27 Jun 2017 15:43:03: 22000000 INFO @ Tue, 27 Jun 2017 15:43:03: 18000000 INFO @ Tue, 27 Jun 2017 15:43:07: 20000000 INFO @ Tue, 27 Jun 2017 15:43:09: 23000000 INFO @ Tue, 27 Jun 2017 15:43:11: 19000000 INFO @ Tue, 27 Jun 2017 15:43:14: 21000000 INFO @ Tue, 27 Jun 2017 15:43:16: 24000000 INFO @ Tue, 27 Jun 2017 15:43:18: 20000000 INFO @ Tue, 27 Jun 2017 15:43:21: 22000000 INFO @ Tue, 27 Jun 2017 15:43:22: 25000000 INFO @ Tue, 27 Jun 2017 15:43:26: 21000000 INFO @ Tue, 27 Jun 2017 15:43:28: 23000000 INFO @ Tue, 27 Jun 2017 15:43:28: 26000000 INFO @ Tue, 27 Jun 2017 15:43:33: 22000000 INFO @ Tue, 27 Jun 2017 15:43:34: 27000000 INFO @ Tue, 27 Jun 2017 15:43:35: 24000000 INFO @ Tue, 27 Jun 2017 15:43:40: 23000000 INFO @ Tue, 27 Jun 2017 15:43:40: 28000000 INFO @ Tue, 27 Jun 2017 15:43:42: 25000000 INFO @ Tue, 27 Jun 2017 15:43:47: 29000000 INFO @ Tue, 27 Jun 2017 15:43:47: 24000000 INFO @ Tue, 27 Jun 2017 15:43:48: 26000000 INFO @ Tue, 27 Jun 2017 15:43:53: 30000000 INFO @ Tue, 27 Jun 2017 15:43:54: 25000000 INFO @ Tue, 27 Jun 2017 15:43:55: 27000000 INFO @ Tue, 27 Jun 2017 15:43:59: 31000000 INFO @ Tue, 27 Jun 2017 15:44:01: 26000000 INFO @ Tue, 27 Jun 2017 15:44:02: 28000000 INFO @ Tue, 27 Jun 2017 15:44:05: 32000000 INFO @ Tue, 27 Jun 2017 15:44:08: 29000000 INFO @ Tue, 27 Jun 2017 15:44:08: 27000000 INFO @ Tue, 27 Jun 2017 15:44:11: 33000000 INFO @ Tue, 27 Jun 2017 15:44:15: 30000000 INFO @ Tue, 27 Jun 2017 15:44:16: 28000000 INFO @ Tue, 27 Jun 2017 15:44:17: 34000000 INFO @ Tue, 27 Jun 2017 15:44:21: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:44:21: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:44:21: #1 total tags in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:44:21: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:44:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:44:22: #1 tags after filtering in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:44:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:44:22: #1 finished! INFO @ Tue, 27 Jun 2017 15:44:22: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:44:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:44:22: 31000000 INFO @ Tue, 27 Jun 2017 15:44:23: 29000000 INFO @ Tue, 27 Jun 2017 15:44:24: #2 number of paired peaks: 72 WARNING @ Tue, 27 Jun 2017 15:44:24: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:44:24: Process for pairing-model is terminated! cat: SRX101815.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX101815.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:44:29: 32000000 INFO @ Tue, 27 Jun 2017 15:44:31: 30000000 INFO @ Tue, 27 Jun 2017 15:44:36: 33000000 INFO @ Tue, 27 Jun 2017 15:44:38: 31000000 INFO @ Tue, 27 Jun 2017 15:44:43: 34000000 INFO @ Tue, 27 Jun 2017 15:44:46: 32000000 INFO @ Tue, 27 Jun 2017 15:44:47: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:44:47: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:44:47: #1 total tags in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:44:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:44:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:44:48: #1 tags after filtering in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:44:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:44:48: #1 finished! INFO @ Tue, 27 Jun 2017 15:44:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:44:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:44:51: #2 number of paired peaks: 72 WARNING @ Tue, 27 Jun 2017 15:44:51: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:44:51: Process for pairing-model is terminated! cat: SRX101815.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX101815.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:44:52: 33000000 INFO @ Tue, 27 Jun 2017 15:44:58: 34000000 INFO @ Tue, 27 Jun 2017 15:45:03: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:45:03: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:45:03: #1 total tags in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:45:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:45:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:45:03: #1 tags after filtering in treatment: 34588697 INFO @ Tue, 27 Jun 2017 15:45:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:45:03: #1 finished! INFO @ Tue, 27 Jun 2017 15:45:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:45:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:45:06: #2 number of paired peaks: 72 WARNING @ Tue, 27 Jun 2017 15:45:06: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:45:06: Process for pairing-model is terminated! cat: SRX101815.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX101815.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX101815.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。