Job ID = 14172052 SRX = SRX10089754 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25355164 spots for SRR13700641/SRR13700641.sra Written 25355164 spots for SRR13700641/SRR13700641.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172519 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:24 25355164 reads; of these: 25355164 (100.00%) were unpaired; of these: 2766492 (10.91%) aligned 0 times 18818455 (74.22%) aligned exactly 1 time 3770217 (14.87%) aligned >1 times 89.09% overall alignment rate Time searching: 00:08:24 Overall time: 00:08:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5301535 / 22588672 = 0.2347 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:08:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:08:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:08:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:08:17: 1000000 INFO @ Sat, 11 Dec 2021 14:08:23: 2000000 INFO @ Sat, 11 Dec 2021 14:08:28: 3000000 INFO @ Sat, 11 Dec 2021 14:08:33: 4000000 INFO @ Sat, 11 Dec 2021 14:08:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:08:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:08:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:08:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:08:44: 6000000 INFO @ Sat, 11 Dec 2021 14:08:46: 1000000 INFO @ Sat, 11 Dec 2021 14:08:50: 7000000 INFO @ Sat, 11 Dec 2021 14:08:52: 2000000 INFO @ Sat, 11 Dec 2021 14:08:56: 8000000 INFO @ Sat, 11 Dec 2021 14:08:57: 3000000 INFO @ Sat, 11 Dec 2021 14:09:01: 9000000 INFO @ Sat, 11 Dec 2021 14:09:03: 4000000 INFO @ Sat, 11 Dec 2021 14:09:07: 10000000 INFO @ Sat, 11 Dec 2021 14:09:09: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:09:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:09:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:09:12: 11000000 INFO @ Sat, 11 Dec 2021 14:09:14: 6000000 INFO @ Sat, 11 Dec 2021 14:09:16: 1000000 INFO @ Sat, 11 Dec 2021 14:09:18: 12000000 INFO @ Sat, 11 Dec 2021 14:09:20: 7000000 INFO @ Sat, 11 Dec 2021 14:09:22: 2000000 INFO @ Sat, 11 Dec 2021 14:09:24: 13000000 INFO @ Sat, 11 Dec 2021 14:09:26: 8000000 INFO @ Sat, 11 Dec 2021 14:09:28: 3000000 INFO @ Sat, 11 Dec 2021 14:09:29: 14000000 INFO @ Sat, 11 Dec 2021 14:09:31: 9000000 INFO @ Sat, 11 Dec 2021 14:09:33: 4000000 INFO @ Sat, 11 Dec 2021 14:09:35: 15000000 INFO @ Sat, 11 Dec 2021 14:09:37: 10000000 INFO @ Sat, 11 Dec 2021 14:09:39: 5000000 INFO @ Sat, 11 Dec 2021 14:09:41: 16000000 INFO @ Sat, 11 Dec 2021 14:09:43: 11000000 INFO @ Sat, 11 Dec 2021 14:09:45: 6000000 INFO @ Sat, 11 Dec 2021 14:09:46: 17000000 INFO @ Sat, 11 Dec 2021 14:09:48: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:09:48: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:09:48: #1 total tags in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:09:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:09:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:09:48: 12000000 INFO @ Sat, 11 Dec 2021 14:09:48: #1 tags after filtering in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:09:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:09:48: #1 finished! INFO @ Sat, 11 Dec 2021 14:09:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:09:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:09:50: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 14:09:50: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:09:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:09:50: 7000000 INFO @ Sat, 11 Dec 2021 14:09:54: 13000000 INFO @ Sat, 11 Dec 2021 14:09:56: 8000000 INFO @ Sat, 11 Dec 2021 14:09:59: 14000000 INFO @ Sat, 11 Dec 2021 14:10:02: 9000000 INFO @ Sat, 11 Dec 2021 14:10:05: 15000000 INFO @ Sat, 11 Dec 2021 14:10:07: 10000000 INFO @ Sat, 11 Dec 2021 14:10:10: 16000000 INFO @ Sat, 11 Dec 2021 14:10:13: 11000000 INFO @ Sat, 11 Dec 2021 14:10:16: 17000000 INFO @ Sat, 11 Dec 2021 14:10:17: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:10:17: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:10:17: #1 total tags in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:10:17: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:10:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:10:18: #1 tags after filtering in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:10:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:10:18: #1 finished! INFO @ Sat, 11 Dec 2021 14:10:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:10:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:10:18: 12000000 INFO @ Sat, 11 Dec 2021 14:10:19: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 14:10:19: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:10:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:10:24: 13000000 INFO @ Sat, 11 Dec 2021 14:10:29: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:10:35: 15000000 INFO @ Sat, 11 Dec 2021 14:10:40: 16000000 INFO @ Sat, 11 Dec 2021 14:10:45: 17000000 INFO @ Sat, 11 Dec 2021 14:10:47: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:10:47: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:10:47: #1 total tags in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:10:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:10:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:10:47: #1 tags after filtering in treatment: 17287137 INFO @ Sat, 11 Dec 2021 14:10:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:10:47: #1 finished! INFO @ Sat, 11 Dec 2021 14:10:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:10:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:10:48: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 14:10:48: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:10:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089754/SRX10089754.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。