Job ID = 9158121 sra ファイルのダウンロード中... Completed: 1922975K bytes transferred in 20 seconds (770957K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22026834 spots for /home/okishinya/chipatlas/results/dm3/SRX099861/SRR350870.sra Written 22026834 spots total Written 40753322 spots for /home/okishinya/chipatlas/results/dm3/SRX099861/SRR350871.sra Written 40753322 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:25 62780156 reads; of these: 62780156 (100.00%) were unpaired; of these: 7866989 (12.53%) aligned 0 times 45686334 (72.77%) aligned exactly 1 time 9226833 (14.70%) aligned >1 times 87.47% overall alignment rate Time searching: 00:18:25 Overall time: 00:18:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 18602501 / 54913167 = 0.3388 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:42:13: # Command line: callpeak -t SRX099861.bam -f BAM -g dm -n SRX099861.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX099861.20 # format = BAM # ChIP-seq file = ['SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:42:13: # Command line: callpeak -t SRX099861.bam -f BAM -g dm -n SRX099861.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX099861.05 # format = BAM # ChIP-seq file = ['SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:42:13: # Command line: callpeak -t SRX099861.bam -f BAM -g dm -n SRX099861.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX099861.10 # format = BAM # ChIP-seq file = ['SRX099861.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:42:13: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:42:13: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:42:13: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:42:13: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:42:13: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:42:13: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:42:19: 1000000 INFO @ Tue, 27 Jun 2017 15:42:20: 1000000 INFO @ Tue, 27 Jun 2017 15:42:20: 1000000 INFO @ Tue, 27 Jun 2017 15:42:25: 2000000 INFO @ Tue, 27 Jun 2017 15:42:27: 2000000 INFO @ Tue, 27 Jun 2017 15:42:27: 2000000 INFO @ Tue, 27 Jun 2017 15:42:31: 3000000 INFO @ Tue, 27 Jun 2017 15:42:34: 3000000 INFO @ Tue, 27 Jun 2017 15:42:35: 3000000 INFO @ Tue, 27 Jun 2017 15:42:38: 4000000 INFO @ Tue, 27 Jun 2017 15:42:41: 4000000 INFO @ Tue, 27 Jun 2017 15:42:42: 4000000 INFO @ Tue, 27 Jun 2017 15:42:44: 5000000 INFO @ Tue, 27 Jun 2017 15:42:47: 5000000 INFO @ Tue, 27 Jun 2017 15:42:49: 5000000 INFO @ Tue, 27 Jun 2017 15:42:50: 6000000 INFO @ Tue, 27 Jun 2017 15:42:54: 6000000 INFO @ Tue, 27 Jun 2017 15:42:56: 7000000 INFO @ Tue, 27 Jun 2017 15:42:57: 6000000 INFO @ Tue, 27 Jun 2017 15:43:01: 7000000 INFO @ Tue, 27 Jun 2017 15:43:02: 8000000 INFO @ Tue, 27 Jun 2017 15:43:04: 7000000 INFO @ Tue, 27 Jun 2017 15:43:08: 9000000 INFO @ Tue, 27 Jun 2017 15:43:08: 8000000 INFO @ Tue, 27 Jun 2017 15:43:11: 8000000 INFO @ Tue, 27 Jun 2017 15:43:14: 10000000 INFO @ Tue, 27 Jun 2017 15:43:15: 9000000 INFO @ Tue, 27 Jun 2017 15:43:18: 9000000 INFO @ Tue, 27 Jun 2017 15:43:19: 11000000 INFO @ Tue, 27 Jun 2017 15:43:22: 10000000 INFO @ Tue, 27 Jun 2017 15:43:25: 10000000 INFO @ Tue, 27 Jun 2017 15:43:25: 12000000 INFO @ Tue, 27 Jun 2017 15:43:29: 11000000 INFO @ Tue, 27 Jun 2017 15:43:31: 13000000 INFO @ Tue, 27 Jun 2017 15:43:32: 11000000 INFO @ Tue, 27 Jun 2017 15:43:35: 12000000 INFO @ Tue, 27 Jun 2017 15:43:37: 14000000 INFO @ Tue, 27 Jun 2017 15:43:39: 12000000 INFO @ Tue, 27 Jun 2017 15:43:42: 13000000 INFO @ Tue, 27 Jun 2017 15:43:43: 15000000 INFO @ Tue, 27 Jun 2017 15:43:46: 13000000 INFO @ Tue, 27 Jun 2017 15:43:49: 14000000 INFO @ Tue, 27 Jun 2017 15:43:49: 16000000 INFO @ Tue, 27 Jun 2017 15:43:53: 14000000 INFO @ Tue, 27 Jun 2017 15:43:55: 17000000 INFO @ Tue, 27 Jun 2017 15:43:55: 15000000 INFO @ Tue, 27 Jun 2017 15:44:00: 15000000 INFO @ Tue, 27 Jun 2017 15:44:01: 18000000 INFO @ Tue, 27 Jun 2017 15:44:02: 16000000 INFO @ Tue, 27 Jun 2017 15:44:06: 16000000 INFO @ Tue, 27 Jun 2017 15:44:07: 19000000 INFO @ Tue, 27 Jun 2017 15:44:09: 17000000 INFO @ Tue, 27 Jun 2017 15:44:13: 20000000 INFO @ Tue, 27 Jun 2017 15:44:13: 17000000 INFO @ Tue, 27 Jun 2017 15:44:15: 18000000 INFO @ Tue, 27 Jun 2017 15:44:19: 21000000 INFO @ Tue, 27 Jun 2017 15:44:20: 18000000 INFO @ Tue, 27 Jun 2017 15:44:22: 19000000 INFO @ Tue, 27 Jun 2017 15:44:26: 22000000 INFO @ Tue, 27 Jun 2017 15:44:28: 19000000 INFO @ Tue, 27 Jun 2017 15:44:29: 20000000 INFO @ Tue, 27 Jun 2017 15:44:32: 23000000 INFO @ Tue, 27 Jun 2017 15:44:34: 20000000 INFO @ Tue, 27 Jun 2017 15:44:36: 21000000 INFO @ Tue, 27 Jun 2017 15:44:38: 24000000 INFO @ Tue, 27 Jun 2017 15:44:42: 21000000 INFO @ Tue, 27 Jun 2017 15:44:43: 22000000 INFO @ Tue, 27 Jun 2017 15:44:44: 25000000 INFO @ Tue, 27 Jun 2017 15:44:49: 22000000 INFO @ Tue, 27 Jun 2017 15:44:49: 23000000 INFO @ Tue, 27 Jun 2017 15:44:50: 26000000 INFO @ Tue, 27 Jun 2017 15:44:56: 23000000 INFO @ Tue, 27 Jun 2017 15:44:56: 27000000 INFO @ Tue, 27 Jun 2017 15:44:56: 24000000 INFO @ Tue, 27 Jun 2017 15:45:02: 28000000 INFO @ Tue, 27 Jun 2017 15:45:03: 25000000 INFO @ Tue, 27 Jun 2017 15:45:03: 24000000 INFO @ Tue, 27 Jun 2017 15:45:08: 29000000 INFO @ Tue, 27 Jun 2017 15:45:10: 26000000 INFO @ Tue, 27 Jun 2017 15:45:10: 25000000 INFO @ Tue, 27 Jun 2017 15:45:14: 30000000 INFO @ Tue, 27 Jun 2017 15:45:17: 27000000 INFO @ Tue, 27 Jun 2017 15:45:17: 26000000 INFO @ Tue, 27 Jun 2017 15:45:20: 31000000 INFO @ Tue, 27 Jun 2017 15:45:23: 28000000 INFO @ Tue, 27 Jun 2017 15:45:24: 27000000 INFO @ Tue, 27 Jun 2017 15:45:26: 32000000 INFO @ Tue, 27 Jun 2017 15:45:30: 29000000 INFO @ Tue, 27 Jun 2017 15:45:32: 28000000 INFO @ Tue, 27 Jun 2017 15:45:32: 33000000 INFO @ Tue, 27 Jun 2017 15:45:37: 30000000 INFO @ Tue, 27 Jun 2017 15:45:38: 34000000 INFO @ Tue, 27 Jun 2017 15:45:39: 29000000 INFO @ Tue, 27 Jun 2017 15:45:44: 31000000 INFO @ Tue, 27 Jun 2017 15:45:45: 35000000 INFO @ Tue, 27 Jun 2017 15:45:46: 30000000 INFO @ Tue, 27 Jun 2017 15:45:50: 32000000 INFO @ Tue, 27 Jun 2017 15:45:51: 36000000 INFO @ Tue, 27 Jun 2017 15:45:53: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:45:53: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:45:53: #1 total tags in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:45:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:45:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:45:53: 31000000 INFO @ Tue, 27 Jun 2017 15:45:54: #1 tags after filtering in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:45:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:45:54: #1 finished! INFO @ Tue, 27 Jun 2017 15:45:54: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:45:57: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 15:45:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:45:57: Process for pairing-model is terminated! cat: SRX099861.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX099861.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:45:57: 33000000 INFO @ Tue, 27 Jun 2017 15:46:00: 32000000 INFO @ Tue, 27 Jun 2017 15:46:03: 34000000 INFO @ Tue, 27 Jun 2017 15:46:06: 33000000 INFO @ Tue, 27 Jun 2017 15:46:09: 35000000 INFO @ Tue, 27 Jun 2017 15:46:12: 34000000 INFO @ Tue, 27 Jun 2017 15:46:15: 36000000 INFO @ Tue, 27 Jun 2017 15:46:18: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:46:18: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:46:18: #1 total tags in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:46:18: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:46:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:46:18: 35000000 INFO @ Tue, 27 Jun 2017 15:46:18: #1 tags after filtering in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:46:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:46:18: #1 finished! INFO @ Tue, 27 Jun 2017 15:46:18: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:46:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:46:21: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 15:46:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:46:21: Process for pairing-model is terminated! cat: SRX099861.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX099861.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:46:24: 36000000 INFO @ Tue, 27 Jun 2017 15:46:27: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 15:46:27: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 15:46:27: #1 total tags in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:46:27: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:46:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:46:27: #1 tags after filtering in treatment: 36310666 INFO @ Tue, 27 Jun 2017 15:46:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:46:27: #1 finished! INFO @ Tue, 27 Jun 2017 15:46:27: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:46:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:46:30: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 15:46:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:46:30: Process for pairing-model is terminated! cat: SRX099861.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX099861.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX099861.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。