Job ID = 9158116 sra ファイルのダウンロード中... Completed: 525583K bytes transferred in 7 seconds (589528K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29974399 spots for /home/okishinya/chipatlas/results/dm3/SRX062284/SRR203280.sra Written 29974399 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:42 29974399 reads; of these: 29974399 (100.00%) were unpaired; of these: 1281622 (4.28%) aligned 0 times 20435600 (68.18%) aligned exactly 1 time 8257177 (27.55%) aligned >1 times 95.72% overall alignment rate Time searching: 00:10:42 Overall time: 00:10:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4720723 / 28692777 = 0.1645 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:25:07: # Command line: callpeak -t SRX062284.bam -f BAM -g dm -n SRX062284.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX062284.05 # format = BAM # ChIP-seq file = ['SRX062284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:25:07: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:25:07: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:25:07: # Command line: callpeak -t SRX062284.bam -f BAM -g dm -n SRX062284.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX062284.10 # format = BAM # ChIP-seq file = ['SRX062284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:25:07: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:25:07: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:25:07: # Command line: callpeak -t SRX062284.bam -f BAM -g dm -n SRX062284.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX062284.20 # format = BAM # ChIP-seq file = ['SRX062284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:25:07: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:25:07: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:25:14: 1000000 INFO @ Tue, 27 Jun 2017 15:25:14: 1000000 INFO @ Tue, 27 Jun 2017 15:25:14: 1000000 INFO @ Tue, 27 Jun 2017 15:25:21: 2000000 INFO @ Tue, 27 Jun 2017 15:25:21: 2000000 INFO @ Tue, 27 Jun 2017 15:25:21: 2000000 INFO @ Tue, 27 Jun 2017 15:25:28: 3000000 INFO @ Tue, 27 Jun 2017 15:25:28: 3000000 INFO @ Tue, 27 Jun 2017 15:25:29: 3000000 INFO @ Tue, 27 Jun 2017 15:25:36: 4000000 INFO @ Tue, 27 Jun 2017 15:25:36: 4000000 INFO @ Tue, 27 Jun 2017 15:25:36: 4000000 INFO @ Tue, 27 Jun 2017 15:25:43: 5000000 INFO @ Tue, 27 Jun 2017 15:25:43: 5000000 INFO @ Tue, 27 Jun 2017 15:25:44: 5000000 INFO @ Tue, 27 Jun 2017 15:25:50: 6000000 INFO @ Tue, 27 Jun 2017 15:25:50: 6000000 INFO @ Tue, 27 Jun 2017 15:25:51: 6000000 INFO @ Tue, 27 Jun 2017 15:25:56: 7000000 INFO @ Tue, 27 Jun 2017 15:25:57: 7000000 INFO @ Tue, 27 Jun 2017 15:25:58: 7000000 INFO @ Tue, 27 Jun 2017 15:26:03: 8000000 INFO @ Tue, 27 Jun 2017 15:26:03: 8000000 INFO @ Tue, 27 Jun 2017 15:26:05: 8000000 INFO @ Tue, 27 Jun 2017 15:26:10: 9000000 INFO @ Tue, 27 Jun 2017 15:26:10: 9000000 INFO @ Tue, 27 Jun 2017 15:26:12: 9000000 INFO @ Tue, 27 Jun 2017 15:26:17: 10000000 INFO @ Tue, 27 Jun 2017 15:26:17: 10000000 INFO @ Tue, 27 Jun 2017 15:26:19: 10000000 INFO @ Tue, 27 Jun 2017 15:26:24: 11000000 INFO @ Tue, 27 Jun 2017 15:26:24: 11000000 INFO @ Tue, 27 Jun 2017 15:26:26: 11000000 INFO @ Tue, 27 Jun 2017 15:26:31: 12000000 INFO @ Tue, 27 Jun 2017 15:26:31: 12000000 INFO @ Tue, 27 Jun 2017 15:26:34: 12000000 INFO @ Tue, 27 Jun 2017 15:26:38: 13000000 INFO @ Tue, 27 Jun 2017 15:26:38: 13000000 INFO @ Tue, 27 Jun 2017 15:26:41: 13000000 INFO @ Tue, 27 Jun 2017 15:26:45: 14000000 INFO @ Tue, 27 Jun 2017 15:26:45: 14000000 INFO @ Tue, 27 Jun 2017 15:26:48: 14000000 INFO @ Tue, 27 Jun 2017 15:26:51: 15000000 INFO @ Tue, 27 Jun 2017 15:26:52: 15000000 INFO @ Tue, 27 Jun 2017 15:26:55: 15000000 INFO @ Tue, 27 Jun 2017 15:26:58: 16000000 INFO @ Tue, 27 Jun 2017 15:26:59: 16000000 INFO @ Tue, 27 Jun 2017 15:27:02: 16000000 INFO @ Tue, 27 Jun 2017 15:27:05: 17000000 INFO @ Tue, 27 Jun 2017 15:27:06: 17000000 INFO @ Tue, 27 Jun 2017 15:27:09: 17000000 INFO @ Tue, 27 Jun 2017 15:27:12: 18000000 INFO @ Tue, 27 Jun 2017 15:27:13: 18000000 INFO @ Tue, 27 Jun 2017 15:27:16: 18000000 INFO @ Tue, 27 Jun 2017 15:27:19: 19000000 INFO @ Tue, 27 Jun 2017 15:27:20: 19000000 INFO @ Tue, 27 Jun 2017 15:27:23: 19000000 INFO @ Tue, 27 Jun 2017 15:27:26: 20000000 INFO @ Tue, 27 Jun 2017 15:27:27: 20000000 INFO @ Tue, 27 Jun 2017 15:27:30: 20000000 INFO @ Tue, 27 Jun 2017 15:27:33: 21000000 INFO @ Tue, 27 Jun 2017 15:27:34: 21000000 INFO @ Tue, 27 Jun 2017 15:27:38: 21000000 INFO @ Tue, 27 Jun 2017 15:27:40: 22000000 INFO @ Tue, 27 Jun 2017 15:27:41: 22000000 INFO @ Tue, 27 Jun 2017 15:27:45: 22000000 INFO @ Tue, 27 Jun 2017 15:27:47: 23000000 INFO @ Tue, 27 Jun 2017 15:27:48: 23000000 INFO @ Tue, 27 Jun 2017 15:27:52: 23000000 INFO @ Tue, 27 Jun 2017 15:27:54: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:27:54: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:27:54: #1 total tags in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:27:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:27:55: #1 tags after filtering in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:27:55: #1 finished! INFO @ Tue, 27 Jun 2017 15:27:55: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:27:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:27:55: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:27:55: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:27:55: #1 total tags in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:55: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:27:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:27:55: #1 tags after filtering in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:27:55: #1 finished! INFO @ Tue, 27 Jun 2017 15:27:55: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:27:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:27:56: #2 number of paired peaks: 70 WARNING @ Tue, 27 Jun 2017 15:27:56: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:27:56: Process for pairing-model is terminated! cat: SRX062284.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062284.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:27:57: #2 number of paired peaks: 70 WARNING @ Tue, 27 Jun 2017 15:27:57: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:27:57: Process for pairing-model is terminated! cat: SRX062284.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062284.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:27:59: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:27:59: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:27:59: #1 total tags in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:59: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:27:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:27:59: #1 tags after filtering in treatment: 23972054 INFO @ Tue, 27 Jun 2017 15:27:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:27:59: #1 finished! INFO @ Tue, 27 Jun 2017 15:27:59: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:27:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:28:01: #2 number of paired peaks: 70 WARNING @ Tue, 27 Jun 2017 15:28:01: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:28:01: Process for pairing-model is terminated! cat: SRX062284.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062284.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062284.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。