Job ID = 9158114 sra ファイルのダウンロード中... Completed: 409300K bytes transferred in 5 seconds (583461K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21869791 spots for /home/okishinya/chipatlas/results/dm3/SRX050609/SRR139091.sra Written 21869791 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:29 21869791 reads; of these: 21869791 (100.00%) were unpaired; of these: 2119494 (9.69%) aligned 0 times 17861281 (81.67%) aligned exactly 1 time 1889016 (8.64%) aligned >1 times 90.31% overall alignment rate Time searching: 00:04:29 Overall time: 00:04:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2850483 / 19750297 = 0.1443 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:16:03: # Command line: callpeak -t SRX050609.bam -f BAM -g dm -n SRX050609.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX050609.20 # format = BAM # ChIP-seq file = ['SRX050609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:16:03: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:16:03: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:16:03: # Command line: callpeak -t SRX050609.bam -f BAM -g dm -n SRX050609.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX050609.05 # format = BAM # ChIP-seq file = ['SRX050609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:16:03: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:16:03: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:16:03: # Command line: callpeak -t SRX050609.bam -f BAM -g dm -n SRX050609.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX050609.10 # format = BAM # ChIP-seq file = ['SRX050609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:16:03: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:16:03: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:16:09: 1000000 INFO @ Tue, 27 Jun 2017 15:16:09: 1000000 INFO @ Tue, 27 Jun 2017 15:16:09: 1000000 INFO @ Tue, 27 Jun 2017 15:16:14: 2000000 INFO @ Tue, 27 Jun 2017 15:16:15: 2000000 INFO @ Tue, 27 Jun 2017 15:16:15: 2000000 INFO @ Tue, 27 Jun 2017 15:16:20: 3000000 INFO @ Tue, 27 Jun 2017 15:16:20: 3000000 INFO @ Tue, 27 Jun 2017 15:16:21: 3000000 INFO @ Tue, 27 Jun 2017 15:16:26: 4000000 INFO @ Tue, 27 Jun 2017 15:16:26: 4000000 INFO @ Tue, 27 Jun 2017 15:16:27: 4000000 INFO @ Tue, 27 Jun 2017 15:16:32: 5000000 INFO @ Tue, 27 Jun 2017 15:16:32: 5000000 INFO @ Tue, 27 Jun 2017 15:16:33: 5000000 INFO @ Tue, 27 Jun 2017 15:16:38: 6000000 INFO @ Tue, 27 Jun 2017 15:16:38: 6000000 INFO @ Tue, 27 Jun 2017 15:16:39: 6000000 INFO @ Tue, 27 Jun 2017 15:16:44: 7000000 INFO @ Tue, 27 Jun 2017 15:16:44: 7000000 INFO @ Tue, 27 Jun 2017 15:16:45: 7000000 INFO @ Tue, 27 Jun 2017 15:16:50: 8000000 INFO @ Tue, 27 Jun 2017 15:16:50: 8000000 INFO @ Tue, 27 Jun 2017 15:16:52: 8000000 INFO @ Tue, 27 Jun 2017 15:16:55: 9000000 INFO @ Tue, 27 Jun 2017 15:16:56: 9000000 INFO @ Tue, 27 Jun 2017 15:16:58: 9000000 INFO @ Tue, 27 Jun 2017 15:17:01: 10000000 INFO @ Tue, 27 Jun 2017 15:17:02: 10000000 INFO @ Tue, 27 Jun 2017 15:17:04: 10000000 INFO @ Tue, 27 Jun 2017 15:17:07: 11000000 INFO @ Tue, 27 Jun 2017 15:17:08: 11000000 INFO @ Tue, 27 Jun 2017 15:17:10: 11000000 INFO @ Tue, 27 Jun 2017 15:17:12: 12000000 INFO @ Tue, 27 Jun 2017 15:17:14: 12000000 INFO @ Tue, 27 Jun 2017 15:17:16: 12000000 INFO @ Tue, 27 Jun 2017 15:17:18: 13000000 INFO @ Tue, 27 Jun 2017 15:17:19: 13000000 INFO @ Tue, 27 Jun 2017 15:17:22: 13000000 INFO @ Tue, 27 Jun 2017 15:17:24: 14000000 INFO @ Tue, 27 Jun 2017 15:17:25: 14000000 INFO @ Tue, 27 Jun 2017 15:17:28: 14000000 INFO @ Tue, 27 Jun 2017 15:17:30: 15000000 INFO @ Tue, 27 Jun 2017 15:17:31: 15000000 INFO @ Tue, 27 Jun 2017 15:17:35: 15000000 INFO @ Tue, 27 Jun 2017 15:17:35: 16000000 INFO @ Tue, 27 Jun 2017 15:17:37: 16000000 INFO @ Tue, 27 Jun 2017 15:17:41: 16000000 INFO @ Tue, 27 Jun 2017 15:17:41: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:41: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:41: #1 total tags in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:41: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:41: #1 tags after filtering in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:41: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:41: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:42: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 15:17:42: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:42: Process for pairing-model is terminated! cat: SRX050609.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050609.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:43: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:43: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:43: #1 total tags in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:43: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:44: #1 tags after filtering in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:44: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:44: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:45: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 15:17:45: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:45: Process for pairing-model is terminated! cat: SRX050609.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050609.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:47: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:47: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:47: #1 total tags in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:48: #1 tags after filtering in treatment: 16899814 INFO @ Tue, 27 Jun 2017 15:17:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:48: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:49: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 15:17:49: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:49: Process for pairing-model is terminated! cat: SRX050609.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050609.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050609.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。