Job ID = 9158113 sra ファイルのダウンロード中... Completed: 381782K bytes transferred in 6 seconds (497616K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20877767 spots for /home/okishinya/chipatlas/results/dm3/SRX050607/SRR139089.sra Written 20877767 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:27 20877767 reads; of these: 20877767 (100.00%) were unpaired; of these: 2136970 (10.24%) aligned 0 times 17351928 (83.11%) aligned exactly 1 time 1388869 (6.65%) aligned >1 times 89.76% overall alignment rate Time searching: 00:04:27 Overall time: 00:04:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2776728 / 18740797 = 0.1482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:15:40: # Command line: callpeak -t SRX050607.bam -f BAM -g dm -n SRX050607.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX050607.20 # format = BAM # ChIP-seq file = ['SRX050607.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:15:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:15:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:15:40: # Command line: callpeak -t SRX050607.bam -f BAM -g dm -n SRX050607.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX050607.05 # format = BAM # ChIP-seq file = ['SRX050607.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:15:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:15:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:15:40: # Command line: callpeak -t SRX050607.bam -f BAM -g dm -n SRX050607.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX050607.10 # format = BAM # ChIP-seq file = ['SRX050607.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:15:40: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:15:40: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:15:46: 1000000 INFO @ Tue, 27 Jun 2017 15:15:47: 1000000 INFO @ Tue, 27 Jun 2017 15:15:48: 1000000 INFO @ Tue, 27 Jun 2017 15:15:53: 2000000 INFO @ Tue, 27 Jun 2017 15:15:53: 2000000 INFO @ Tue, 27 Jun 2017 15:15:56: 2000000 INFO @ Tue, 27 Jun 2017 15:16:00: 3000000 INFO @ Tue, 27 Jun 2017 15:16:00: 3000000 INFO @ Tue, 27 Jun 2017 15:16:04: 3000000 INFO @ Tue, 27 Jun 2017 15:16:07: 4000000 INFO @ Tue, 27 Jun 2017 15:16:07: 4000000 INFO @ Tue, 27 Jun 2017 15:16:12: 4000000 INFO @ Tue, 27 Jun 2017 15:16:13: 5000000 INFO @ Tue, 27 Jun 2017 15:16:14: 5000000 INFO @ Tue, 27 Jun 2017 15:16:20: 5000000 INFO @ Tue, 27 Jun 2017 15:16:20: 6000000 INFO @ Tue, 27 Jun 2017 15:16:21: 6000000 INFO @ Tue, 27 Jun 2017 15:16:27: 7000000 INFO @ Tue, 27 Jun 2017 15:16:28: 6000000 INFO @ Tue, 27 Jun 2017 15:16:28: 7000000 INFO @ Tue, 27 Jun 2017 15:16:34: 8000000 INFO @ Tue, 27 Jun 2017 15:16:35: 8000000 INFO @ Tue, 27 Jun 2017 15:16:36: 7000000 INFO @ Tue, 27 Jun 2017 15:16:41: 9000000 INFO @ Tue, 27 Jun 2017 15:16:42: 9000000 INFO @ Tue, 27 Jun 2017 15:16:45: 8000000 INFO @ Tue, 27 Jun 2017 15:16:48: 10000000 INFO @ Tue, 27 Jun 2017 15:16:49: 10000000 INFO @ Tue, 27 Jun 2017 15:16:53: 9000000 INFO @ Tue, 27 Jun 2017 15:16:54: 11000000 INFO @ Tue, 27 Jun 2017 15:16:56: 11000000 INFO @ Tue, 27 Jun 2017 15:17:01: 10000000 INFO @ Tue, 27 Jun 2017 15:17:01: 12000000 INFO @ Tue, 27 Jun 2017 15:17:03: 12000000 INFO @ Tue, 27 Jun 2017 15:17:08: 13000000 INFO @ Tue, 27 Jun 2017 15:17:09: 11000000 INFO @ Tue, 27 Jun 2017 15:17:10: 13000000 INFO @ Tue, 27 Jun 2017 15:17:14: 14000000 INFO @ Tue, 27 Jun 2017 15:17:17: 12000000 INFO @ Tue, 27 Jun 2017 15:17:17: 14000000 INFO @ Tue, 27 Jun 2017 15:17:21: 15000000 INFO @ Tue, 27 Jun 2017 15:17:24: 15000000 INFO @ Tue, 27 Jun 2017 15:17:25: 13000000 INFO @ Tue, 27 Jun 2017 15:17:28: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:28: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:28: #1 total tags in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:28: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:28: #1 tags after filtering in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:28: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:28: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:29: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 15:17:29: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:29: Process for pairing-model is terminated! cat: SRX050607.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050607.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:30: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:30: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:30: #1 total tags in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:31: #1 tags after filtering in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:31: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:32: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 15:17:32: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:32: Process for pairing-model is terminated! cat: SRX050607.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050607.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:32: 14000000 INFO @ Tue, 27 Jun 2017 15:17:39: 15000000 INFO @ Tue, 27 Jun 2017 15:17:46: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:46: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:46: #1 total tags in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:46: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:46: #1 tags after filtering in treatment: 15964069 INFO @ Tue, 27 Jun 2017 15:17:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:46: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:46: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:47: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 15:17:47: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:47: Process for pairing-model is terminated! cat: SRX050607.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX050607.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX050607.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。