Job ID = 6527512 SRX = SRX041395 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:57:08 prefetch.2.10.7: 1) Downloading 'SRR099129'... 2020-06-29T12:57:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:58:34 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:58:34 prefetch.2.10.7: 'SRR099129' is valid 2020-06-29T12:58:34 prefetch.2.10.7: 1) 'SRR099129' was downloaded successfully Read 19660324 spots for SRR099129/SRR099129.sra Written 19660324 spots for SRR099129/SRR099129.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 19660324 reads; of these: 19660324 (100.00%) were unpaired; of these: 806248 (4.10%) aligned 0 times 15736578 (80.04%) aligned exactly 1 time 3117498 (15.86%) aligned >1 times 95.90% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2354156 / 18854076 = 0.1249 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:11:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:11:41: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:11:41: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:11:47: 1000000 INFO @ Mon, 29 Jun 2020 22:11:52: 2000000 INFO @ Mon, 29 Jun 2020 22:11:56: 3000000 INFO @ Mon, 29 Jun 2020 22:12:01: 4000000 INFO @ Mon, 29 Jun 2020 22:12:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:12:11: 6000000 INFO @ Mon, 29 Jun 2020 22:12:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:12:11: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:12:11: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:12:16: 7000000 INFO @ Mon, 29 Jun 2020 22:12:17: 1000000 INFO @ Mon, 29 Jun 2020 22:12:21: 8000000 INFO @ Mon, 29 Jun 2020 22:12:22: 2000000 INFO @ Mon, 29 Jun 2020 22:12:26: 9000000 INFO @ Mon, 29 Jun 2020 22:12:27: 3000000 INFO @ Mon, 29 Jun 2020 22:12:31: 10000000 INFO @ Mon, 29 Jun 2020 22:12:32: 4000000 INFO @ Mon, 29 Jun 2020 22:12:36: 11000000 INFO @ Mon, 29 Jun 2020 22:12:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:12:41: 12000000 INFO @ Mon, 29 Jun 2020 22:12:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:12:41: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:12:41: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:12:42: 6000000 INFO @ Mon, 29 Jun 2020 22:12:47: 13000000 INFO @ Mon, 29 Jun 2020 22:12:47: 1000000 INFO @ Mon, 29 Jun 2020 22:12:48: 7000000 INFO @ Mon, 29 Jun 2020 22:12:52: 14000000 INFO @ Mon, 29 Jun 2020 22:12:53: 2000000 INFO @ Mon, 29 Jun 2020 22:12:53: 8000000 INFO @ Mon, 29 Jun 2020 22:12:58: 15000000 INFO @ Mon, 29 Jun 2020 22:12:58: 3000000 INFO @ Mon, 29 Jun 2020 22:12:59: 9000000 INFO @ Mon, 29 Jun 2020 22:13:03: 4000000 INFO @ Mon, 29 Jun 2020 22:13:03: 16000000 INFO @ Mon, 29 Jun 2020 22:13:04: 10000000 INFO @ Mon, 29 Jun 2020 22:13:06: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:13:06: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:13:06: #1 total tags in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:13:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:06: #1 tags after filtering in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:13:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:06: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:07: #2 number of paired peaks: 91 WARNING @ Mon, 29 Jun 2020 22:13:07: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:13:08: 5000000 INFO @ Mon, 29 Jun 2020 22:13:09: 11000000 INFO @ Mon, 29 Jun 2020 22:13:13: 6000000 INFO @ Mon, 29 Jun 2020 22:13:14: 12000000 INFO @ Mon, 29 Jun 2020 22:13:18: 7000000 INFO @ Mon, 29 Jun 2020 22:13:19: 13000000 INFO @ Mon, 29 Jun 2020 22:13:23: 8000000 INFO @ Mon, 29 Jun 2020 22:13:24: 14000000 INFO @ Mon, 29 Jun 2020 22:13:28: 9000000 INFO @ Mon, 29 Jun 2020 22:13:29: 15000000 INFO @ Mon, 29 Jun 2020 22:13:33: 10000000 INFO @ Mon, 29 Jun 2020 22:13:34: 16000000 INFO @ Mon, 29 Jun 2020 22:13:37: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:13:37: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:13:37: #1 total tags in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:13:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:37: #1 tags after filtering in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:13:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:37: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:37: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:38: #2 number of paired peaks: 91 WARNING @ Mon, 29 Jun 2020 22:13:38: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:13:38: 11000000 INFO @ Mon, 29 Jun 2020 22:13:43: 12000000 INFO @ Mon, 29 Jun 2020 22:13:48: 13000000 INFO @ Mon, 29 Jun 2020 22:13:52: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:13:57: 15000000 INFO @ Mon, 29 Jun 2020 22:14:02: 16000000 INFO @ Mon, 29 Jun 2020 22:14:04: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:14:04: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:14:04: #1 total tags in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:14:04: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:14:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:14:05: #1 tags after filtering in treatment: 16499920 INFO @ Mon, 29 Jun 2020 22:14:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:14:05: #1 finished! INFO @ Mon, 29 Jun 2020 22:14:05: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:14:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:14:06: #2 number of paired peaks: 91 WARNING @ Mon, 29 Jun 2020 22:14:06: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:14:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX041395/SRX041395.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。