Job ID = 9158084 sra ファイルのダウンロード中... Completed: 556655K bytes transferred in 7 seconds (592009K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28434966 spots for /home/okishinya/chipatlas/results/dm3/SRX041383/SRR099111.sra Written 28434966 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:05 28434966 reads; of these: 28434966 (100.00%) were unpaired; of these: 1403706 (4.94%) aligned 0 times 21396769 (75.25%) aligned exactly 1 time 5634491 (19.82%) aligned >1 times 95.06% overall alignment rate Time searching: 00:09:05 Overall time: 00:09:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4358930 / 27031260 = 0.1613 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:14:29: # Command line: callpeak -t SRX041383.bam -f BAM -g dm -n SRX041383.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX041383.10 # format = BAM # ChIP-seq file = ['SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:29: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:29: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:29: # Command line: callpeak -t SRX041383.bam -f BAM -g dm -n SRX041383.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX041383.20 # format = BAM # ChIP-seq file = ['SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:29: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:29: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:29: # Command line: callpeak -t SRX041383.bam -f BAM -g dm -n SRX041383.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX041383.05 # format = BAM # ChIP-seq file = ['SRX041383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:29: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:29: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:36: 1000000 INFO @ Tue, 27 Jun 2017 15:14:36: 1000000 INFO @ Tue, 27 Jun 2017 15:14:36: 1000000 INFO @ Tue, 27 Jun 2017 15:14:42: 2000000 INFO @ Tue, 27 Jun 2017 15:14:43: 2000000 INFO @ Tue, 27 Jun 2017 15:14:43: 2000000 INFO @ Tue, 27 Jun 2017 15:14:49: 3000000 INFO @ Tue, 27 Jun 2017 15:14:49: 3000000 INFO @ Tue, 27 Jun 2017 15:14:51: 3000000 INFO @ Tue, 27 Jun 2017 15:14:56: 4000000 INFO @ Tue, 27 Jun 2017 15:14:56: 4000000 INFO @ Tue, 27 Jun 2017 15:14:57: 4000000 INFO @ Tue, 27 Jun 2017 15:15:03: 5000000 INFO @ Tue, 27 Jun 2017 15:15:04: 5000000 INFO @ Tue, 27 Jun 2017 15:15:05: 5000000 INFO @ Tue, 27 Jun 2017 15:15:09: 6000000 INFO @ Tue, 27 Jun 2017 15:15:12: 6000000 INFO @ Tue, 27 Jun 2017 15:15:12: 6000000 INFO @ Tue, 27 Jun 2017 15:15:16: 7000000 INFO @ Tue, 27 Jun 2017 15:15:19: 7000000 INFO @ Tue, 27 Jun 2017 15:15:19: 7000000 INFO @ Tue, 27 Jun 2017 15:15:23: 8000000 INFO @ Tue, 27 Jun 2017 15:15:26: 8000000 INFO @ Tue, 27 Jun 2017 15:15:27: 8000000 INFO @ Tue, 27 Jun 2017 15:15:29: 9000000 INFO @ Tue, 27 Jun 2017 15:15:33: 9000000 INFO @ Tue, 27 Jun 2017 15:15:34: 9000000 INFO @ Tue, 27 Jun 2017 15:15:36: 10000000 INFO @ Tue, 27 Jun 2017 15:15:40: 10000000 INFO @ Tue, 27 Jun 2017 15:15:42: 10000000 INFO @ Tue, 27 Jun 2017 15:15:43: 11000000 INFO @ Tue, 27 Jun 2017 15:15:47: 11000000 INFO @ Tue, 27 Jun 2017 15:15:49: 12000000 INFO @ Tue, 27 Jun 2017 15:15:50: 11000000 INFO @ Tue, 27 Jun 2017 15:15:55: 12000000 INFO @ Tue, 27 Jun 2017 15:15:56: 13000000 INFO @ Tue, 27 Jun 2017 15:15:58: 12000000 INFO @ Tue, 27 Jun 2017 15:16:02: 13000000 INFO @ Tue, 27 Jun 2017 15:16:02: 14000000 INFO @ Tue, 27 Jun 2017 15:16:06: 13000000 INFO @ Tue, 27 Jun 2017 15:16:09: 15000000 INFO @ Tue, 27 Jun 2017 15:16:09: 14000000 INFO @ Tue, 27 Jun 2017 15:16:14: 14000000 INFO @ Tue, 27 Jun 2017 15:16:15: 16000000 INFO @ Tue, 27 Jun 2017 15:16:16: 15000000 INFO @ Tue, 27 Jun 2017 15:16:22: 17000000 INFO @ Tue, 27 Jun 2017 15:16:22: 15000000 INFO @ Tue, 27 Jun 2017 15:16:24: 16000000 INFO @ Tue, 27 Jun 2017 15:16:28: 18000000 INFO @ Tue, 27 Jun 2017 15:16:30: 16000000 INFO @ Tue, 27 Jun 2017 15:16:31: 17000000 INFO @ Tue, 27 Jun 2017 15:16:35: 19000000 INFO @ Tue, 27 Jun 2017 15:16:38: 17000000 INFO @ Tue, 27 Jun 2017 15:16:39: 18000000 INFO @ Tue, 27 Jun 2017 15:16:41: 20000000 INFO @ Tue, 27 Jun 2017 15:16:46: 19000000 INFO @ Tue, 27 Jun 2017 15:16:46: 18000000 INFO @ Tue, 27 Jun 2017 15:16:48: 21000000 INFO @ Tue, 27 Jun 2017 15:16:53: 20000000 INFO @ Tue, 27 Jun 2017 15:16:54: 19000000 INFO @ Tue, 27 Jun 2017 15:16:55: 22000000 INFO @ Tue, 27 Jun 2017 15:17:00: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:00: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:00: #1 total tags in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:00: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:00: #1 tags after filtering in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:00: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:00: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:01: 21000000 INFO @ Tue, 27 Jun 2017 15:17:02: #2 number of paired peaks: 97 WARNING @ Tue, 27 Jun 2017 15:17:02: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:02: Process for pairing-model is terminated! cat: SRX041383.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX041383.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:03: 20000000 INFO @ Tue, 27 Jun 2017 15:17:08: 22000000 INFO @ Tue, 27 Jun 2017 15:17:11: 21000000 INFO @ Tue, 27 Jun 2017 15:17:14: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:14: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:14: #1 total tags in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:14: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:14: #1 tags after filtering in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:14: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:14: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:15: #2 number of paired peaks: 97 WARNING @ Tue, 27 Jun 2017 15:17:15: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:15: Process for pairing-model is terminated! cat: SRX041383.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX041383.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:18: 22000000 INFO @ Tue, 27 Jun 2017 15:17:23: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:23: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:23: #1 total tags in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:23: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:24: #1 tags after filtering in treatment: 22672330 INFO @ Tue, 27 Jun 2017 15:17:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:24: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:25: #2 number of paired peaks: 97 WARNING @ Tue, 27 Jun 2017 15:17:25: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:25: Process for pairing-model is terminated! cat: SRX041383.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX041383.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX041383.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。