Job ID = 9158065 sra ファイルのダウンロード中... Completed: 589767K bytes transferred in 8 seconds (584465K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 26809915 spots for /home/okishinya/chipatlas/results/dm3/SRX030965/SRR072395.sra Written 26809915 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:45 26809915 reads; of these: 26809915 (100.00%) were unpaired; of these: 8171293 (30.48%) aligned 0 times 15806723 (58.96%) aligned exactly 1 time 2831899 (10.56%) aligned >1 times 69.52% overall alignment rate Time searching: 00:05:45 Overall time: 00:05:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2085491 / 18638622 = 0.1119 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 14:57:55: # Command line: callpeak -t SRX030965.bam -f BAM -g dm -n SRX030965.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX030965.10 # format = BAM # ChIP-seq file = ['SRX030965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:57:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:57:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:57:55: # Command line: callpeak -t SRX030965.bam -f BAM -g dm -n SRX030965.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX030965.05 # format = BAM # ChIP-seq file = ['SRX030965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:57:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:57:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:57:55: # Command line: callpeak -t SRX030965.bam -f BAM -g dm -n SRX030965.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX030965.20 # format = BAM # ChIP-seq file = ['SRX030965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:57:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:57:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:58:03: 1000000 INFO @ Tue, 27 Jun 2017 14:58:03: 1000000 INFO @ Tue, 27 Jun 2017 14:58:03: 1000000 INFO @ Tue, 27 Jun 2017 14:58:11: 2000000 INFO @ Tue, 27 Jun 2017 14:58:11: 2000000 INFO @ Tue, 27 Jun 2017 14:58:12: 2000000 INFO @ Tue, 27 Jun 2017 14:58:18: 3000000 INFO @ Tue, 27 Jun 2017 14:58:20: 3000000 INFO @ Tue, 27 Jun 2017 14:58:20: 3000000 INFO @ Tue, 27 Jun 2017 14:58:26: 4000000 INFO @ Tue, 27 Jun 2017 14:58:28: 4000000 INFO @ Tue, 27 Jun 2017 14:58:28: 4000000 INFO @ Tue, 27 Jun 2017 14:58:34: 5000000 INFO @ Tue, 27 Jun 2017 14:58:36: 5000000 INFO @ Tue, 27 Jun 2017 14:58:37: 5000000 INFO @ Tue, 27 Jun 2017 14:58:41: 6000000 INFO @ Tue, 27 Jun 2017 14:58:45: 6000000 INFO @ Tue, 27 Jun 2017 14:58:45: 6000000 INFO @ Tue, 27 Jun 2017 14:58:48: 7000000 INFO @ Tue, 27 Jun 2017 14:58:53: 7000000 INFO @ Tue, 27 Jun 2017 14:58:53: 7000000 INFO @ Tue, 27 Jun 2017 14:58:56: 8000000 INFO @ Tue, 27 Jun 2017 14:59:01: 8000000 INFO @ Tue, 27 Jun 2017 14:59:01: 8000000 INFO @ Tue, 27 Jun 2017 14:59:03: 9000000 INFO @ Tue, 27 Jun 2017 14:59:09: 9000000 INFO @ Tue, 27 Jun 2017 14:59:09: 9000000 INFO @ Tue, 27 Jun 2017 14:59:10: 10000000 INFO @ Tue, 27 Jun 2017 14:59:17: 10000000 INFO @ Tue, 27 Jun 2017 14:59:17: 10000000 INFO @ Tue, 27 Jun 2017 14:59:18: 11000000 INFO @ Tue, 27 Jun 2017 14:59:25: 12000000 INFO @ Tue, 27 Jun 2017 14:59:26: 11000000 INFO @ Tue, 27 Jun 2017 14:59:26: 11000000 INFO @ Tue, 27 Jun 2017 14:59:32: 13000000 INFO @ Tue, 27 Jun 2017 14:59:34: 12000000 INFO @ Tue, 27 Jun 2017 14:59:34: 12000000 INFO @ Tue, 27 Jun 2017 14:59:40: 14000000 INFO @ Tue, 27 Jun 2017 14:59:42: 13000000 INFO @ Tue, 27 Jun 2017 14:59:42: 13000000 INFO @ Tue, 27 Jun 2017 14:59:47: 15000000 INFO @ Tue, 27 Jun 2017 14:59:50: 14000000 INFO @ Tue, 27 Jun 2017 14:59:50: 14000000 INFO @ Tue, 27 Jun 2017 14:59:54: 16000000 INFO @ Tue, 27 Jun 2017 14:59:58: 15000000 INFO @ Tue, 27 Jun 2017 14:59:59: 15000000 INFO @ Tue, 27 Jun 2017 14:59:59: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 14:59:59: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 14:59:59: #1 total tags in treatment: 16553131 INFO @ Tue, 27 Jun 2017 14:59:59: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:59:59: #1 tags after filtering in treatment: 16553131 INFO @ Tue, 27 Jun 2017 14:59:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:59:59: #1 finished! INFO @ Tue, 27 Jun 2017 14:59:59: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:00:00: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 15:00:00: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:00:00: Process for pairing-model is terminated! cat: SRX030965.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030965.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:00:07: 16000000 INFO @ Tue, 27 Jun 2017 15:00:07: 16000000 INFO @ Tue, 27 Jun 2017 15:00:11: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:00:11: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:00:11: #1 total tags in treatment: 16553131 INFO @ Tue, 27 Jun 2017 15:00:11: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:00:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:00:11: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:00:11: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:00:11: #1 total tags in treatment: 16553131 INFO @ Tue, 27 Jun 2017 15:00:11: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:00:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:00:11: #1 tags after filtering in treatment: 16553131 INFO @ Tue, 27 Jun 2017 15:00:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:00:11: #1 finished! INFO @ Tue, 27 Jun 2017 15:00:11: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:00:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:00:11: #1 tags after filtering in treatment: 16553131 INFO @ Tue, 27 Jun 2017 15:00:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:00:11: #1 finished! INFO @ Tue, 27 Jun 2017 15:00:11: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:00:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:00:12: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 15:00:12: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:00:12: Process for pairing-model is terminated! cat: SRX030965.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030965.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:00:13: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 15:00:13: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:00:13: Process for pairing-model is terminated! cat: SRX030965.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030965.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030965.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。