Job ID = 6527485 SRX = SRX030152 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:52:58 prefetch.2.10.7: 1) Downloading 'SRR071268'... 2020-06-29T12:52:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:55:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:55:05 prefetch.2.10.7: 'SRR071268' is valid 2020-06-29T12:55:05 prefetch.2.10.7: 1) 'SRR071268' was downloaded successfully Read 24829903 spots for SRR071268/SRR071268.sra Written 24829903 spots for SRR071268/SRR071268.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:23 24829903 reads; of these: 24829903 (100.00%) were unpaired; of these: 624717 (2.52%) aligned 0 times 20026894 (80.66%) aligned exactly 1 time 4178292 (16.83%) aligned >1 times 97.48% overall alignment rate Time searching: 00:05:23 Overall time: 00:05:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4234656 / 24205186 = 0.1749 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:10:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:10:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:10:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:10:34: 1000000 INFO @ Mon, 29 Jun 2020 22:10:39: 2000000 INFO @ Mon, 29 Jun 2020 22:10:44: 3000000 INFO @ Mon, 29 Jun 2020 22:10:49: 4000000 INFO @ Mon, 29 Jun 2020 22:10:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:10:59: 6000000 INFO @ Mon, 29 Jun 2020 22:10:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:10:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:10:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:11:05: 7000000 INFO @ Mon, 29 Jun 2020 22:11:05: 1000000 INFO @ Mon, 29 Jun 2020 22:11:10: 8000000 INFO @ Mon, 29 Jun 2020 22:11:12: 2000000 INFO @ Mon, 29 Jun 2020 22:11:16: 9000000 INFO @ Mon, 29 Jun 2020 22:11:18: 3000000 INFO @ Mon, 29 Jun 2020 22:11:22: 10000000 INFO @ Mon, 29 Jun 2020 22:11:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:11:28: 11000000 INFO @ Mon, 29 Jun 2020 22:11:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:11:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:11:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:11:30: 5000000 INFO @ Mon, 29 Jun 2020 22:11:34: 12000000 INFO @ Mon, 29 Jun 2020 22:11:35: 1000000 INFO @ Mon, 29 Jun 2020 22:11:37: 6000000 INFO @ Mon, 29 Jun 2020 22:11:39: 13000000 INFO @ Mon, 29 Jun 2020 22:11:41: 2000000 INFO @ Mon, 29 Jun 2020 22:11:43: 7000000 INFO @ Mon, 29 Jun 2020 22:11:45: 14000000 INFO @ Mon, 29 Jun 2020 22:11:46: 3000000 INFO @ Mon, 29 Jun 2020 22:11:49: 8000000 INFO @ Mon, 29 Jun 2020 22:11:51: 15000000 INFO @ Mon, 29 Jun 2020 22:11:52: 4000000 INFO @ Mon, 29 Jun 2020 22:11:55: 9000000 INFO @ Mon, 29 Jun 2020 22:11:57: 16000000 INFO @ Mon, 29 Jun 2020 22:11:58: 5000000 INFO @ Mon, 29 Jun 2020 22:12:01: 10000000 INFO @ Mon, 29 Jun 2020 22:12:03: 17000000 INFO @ Mon, 29 Jun 2020 22:12:04: 6000000 INFO @ Mon, 29 Jun 2020 22:12:08: 11000000 INFO @ Mon, 29 Jun 2020 22:12:09: 18000000 INFO @ Mon, 29 Jun 2020 22:12:10: 7000000 INFO @ Mon, 29 Jun 2020 22:12:14: 12000000 INFO @ Mon, 29 Jun 2020 22:12:14: 19000000 INFO @ Mon, 29 Jun 2020 22:12:16: 8000000 INFO @ Mon, 29 Jun 2020 22:12:20: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:12:20: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:12:20: #1 total tags in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:12:20: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:12:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:12:20: 13000000 INFO @ Mon, 29 Jun 2020 22:12:20: #1 tags after filtering in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:12:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:12:20: #1 finished! INFO @ Mon, 29 Jun 2020 22:12:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:12:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:12:21: 9000000 INFO @ Mon, 29 Jun 2020 22:12:22: #2 number of paired peaks: 53 WARNING @ Mon, 29 Jun 2020 22:12:22: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:12:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:12:26: 14000000 INFO @ Mon, 29 Jun 2020 22:12:27: 10000000 INFO @ Mon, 29 Jun 2020 22:12:33: 15000000 INFO @ Mon, 29 Jun 2020 22:12:33: 11000000 INFO @ Mon, 29 Jun 2020 22:12:39: 12000000 INFO @ Mon, 29 Jun 2020 22:12:39: 16000000 INFO @ Mon, 29 Jun 2020 22:12:45: 13000000 INFO @ Mon, 29 Jun 2020 22:12:45: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:12:51: 14000000 INFO @ Mon, 29 Jun 2020 22:12:52: 18000000 INFO @ Mon, 29 Jun 2020 22:12:57: 15000000 INFO @ Mon, 29 Jun 2020 22:12:58: 19000000 INFO @ Mon, 29 Jun 2020 22:13:03: 16000000 INFO @ Mon, 29 Jun 2020 22:13:04: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:13:04: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:13:04: #1 total tags in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:13:04: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:04: #1 tags after filtering in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:13:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:04: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:04: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:06: #2 number of paired peaks: 53 WARNING @ Mon, 29 Jun 2020 22:13:06: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:13:08: 17000000 INFO @ Mon, 29 Jun 2020 22:13:13: 18000000 INFO @ Mon, 29 Jun 2020 22:13:19: 19000000 INFO @ Mon, 29 Jun 2020 22:13:24: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:13:24: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:13:24: #1 total tags in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:13:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:24: #1 tags after filtering in treatment: 19970530 INFO @ Mon, 29 Jun 2020 22:13:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:24: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:24: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:25: #2 number of paired peaks: 53 WARNING @ Mon, 29 Jun 2020 22:13:25: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX030152/SRX030152.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。