Job ID = 9158059 sra ファイルのダウンロード中... Completed: 871462K bytes transferred in 11 seconds (648523K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 15541772 spots for /home/okishinya/chipatlas/results/dm3/SRX026869/SRR065771.sra Written 15541772 spots total Written 28134051 spots for /home/okishinya/chipatlas/results/dm3/SRX026869/SRR065772.sra Written 28134051 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:05 43675823 reads; of these: 43675823 (100.00%) were unpaired; of these: 1291261 (2.96%) aligned 0 times 30217422 (69.19%) aligned exactly 1 time 12167140 (27.86%) aligned >1 times 97.04% overall alignment rate Time searching: 00:17:05 Overall time: 00:17:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7741624 / 42384562 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:12:21: # Command line: callpeak -t SRX026869.bam -f BAM -g dm -n SRX026869.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX026869.20 # format = BAM # ChIP-seq file = ['SRX026869.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:12:21: # Command line: callpeak -t SRX026869.bam -f BAM -g dm -n SRX026869.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX026869.05 # format = BAM # ChIP-seq file = ['SRX026869.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:12:21: # Command line: callpeak -t SRX026869.bam -f BAM -g dm -n SRX026869.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX026869.10 # format = BAM # ChIP-seq file = ['SRX026869.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:12:29: 1000000 INFO @ Tue, 27 Jun 2017 15:12:29: 1000000 INFO @ Tue, 27 Jun 2017 15:12:29: 1000000 INFO @ Tue, 27 Jun 2017 15:12:36: 2000000 INFO @ Tue, 27 Jun 2017 15:12:37: 2000000 INFO @ Tue, 27 Jun 2017 15:12:37: 2000000 INFO @ Tue, 27 Jun 2017 15:12:43: 3000000 INFO @ Tue, 27 Jun 2017 15:12:45: 3000000 INFO @ Tue, 27 Jun 2017 15:12:45: 3000000 INFO @ Tue, 27 Jun 2017 15:12:50: 4000000 INFO @ Tue, 27 Jun 2017 15:12:53: 4000000 INFO @ Tue, 27 Jun 2017 15:12:53: 4000000 INFO @ Tue, 27 Jun 2017 15:12:57: 5000000 INFO @ Tue, 27 Jun 2017 15:13:01: 5000000 INFO @ Tue, 27 Jun 2017 15:13:01: 5000000 INFO @ Tue, 27 Jun 2017 15:13:04: 6000000 INFO @ Tue, 27 Jun 2017 15:13:10: 6000000 INFO @ Tue, 27 Jun 2017 15:13:10: 6000000 INFO @ Tue, 27 Jun 2017 15:13:12: 7000000 INFO @ Tue, 27 Jun 2017 15:13:18: 7000000 INFO @ Tue, 27 Jun 2017 15:13:18: 7000000 INFO @ Tue, 27 Jun 2017 15:13:19: 8000000 INFO @ Tue, 27 Jun 2017 15:13:26: 8000000 INFO @ Tue, 27 Jun 2017 15:13:26: 8000000 INFO @ Tue, 27 Jun 2017 15:13:26: 9000000 INFO @ Tue, 27 Jun 2017 15:13:34: 10000000 INFO @ Tue, 27 Jun 2017 15:13:35: 9000000 INFO @ Tue, 27 Jun 2017 15:13:35: 9000000 INFO @ Tue, 27 Jun 2017 15:13:42: 11000000 INFO @ Tue, 27 Jun 2017 15:13:43: 10000000 INFO @ Tue, 27 Jun 2017 15:13:43: 10000000 INFO @ Tue, 27 Jun 2017 15:13:49: 12000000 INFO @ Tue, 27 Jun 2017 15:13:51: 11000000 INFO @ Tue, 27 Jun 2017 15:13:51: 11000000 INFO @ Tue, 27 Jun 2017 15:13:56: 13000000 INFO @ Tue, 27 Jun 2017 15:14:00: 12000000 INFO @ Tue, 27 Jun 2017 15:14:00: 12000000 INFO @ Tue, 27 Jun 2017 15:14:04: 14000000 INFO @ Tue, 27 Jun 2017 15:14:08: 13000000 INFO @ Tue, 27 Jun 2017 15:14:08: 13000000 INFO @ Tue, 27 Jun 2017 15:14:11: 15000000 INFO @ Tue, 27 Jun 2017 15:14:16: 14000000 INFO @ Tue, 27 Jun 2017 15:14:16: 14000000 INFO @ Tue, 27 Jun 2017 15:14:18: 16000000 INFO @ Tue, 27 Jun 2017 15:14:25: 15000000 INFO @ Tue, 27 Jun 2017 15:14:25: 15000000 INFO @ Tue, 27 Jun 2017 15:14:25: 17000000 INFO @ Tue, 27 Jun 2017 15:14:32: 18000000 INFO @ Tue, 27 Jun 2017 15:14:33: 16000000 INFO @ Tue, 27 Jun 2017 15:14:33: 16000000 INFO @ Tue, 27 Jun 2017 15:14:39: 19000000 INFO @ Tue, 27 Jun 2017 15:14:41: 17000000 INFO @ Tue, 27 Jun 2017 15:14:41: 17000000 INFO @ Tue, 27 Jun 2017 15:14:46: 20000000 INFO @ Tue, 27 Jun 2017 15:14:49: 18000000 INFO @ Tue, 27 Jun 2017 15:14:49: 18000000 INFO @ Tue, 27 Jun 2017 15:14:53: 21000000 INFO @ Tue, 27 Jun 2017 15:14:58: 19000000 INFO @ Tue, 27 Jun 2017 15:14:58: 19000000 INFO @ Tue, 27 Jun 2017 15:15:00: 22000000 INFO @ Tue, 27 Jun 2017 15:15:06: 20000000 INFO @ Tue, 27 Jun 2017 15:15:06: 20000000 INFO @ Tue, 27 Jun 2017 15:15:07: 23000000 INFO @ Tue, 27 Jun 2017 15:15:14: 24000000 INFO @ Tue, 27 Jun 2017 15:15:14: 21000000 INFO @ Tue, 27 Jun 2017 15:15:14: 21000000 INFO @ Tue, 27 Jun 2017 15:15:21: 25000000 INFO @ Tue, 27 Jun 2017 15:15:22: 22000000 INFO @ Tue, 27 Jun 2017 15:15:22: 22000000 INFO @ Tue, 27 Jun 2017 15:15:28: 26000000 INFO @ Tue, 27 Jun 2017 15:15:30: 23000000 INFO @ Tue, 27 Jun 2017 15:15:30: 23000000 INFO @ Tue, 27 Jun 2017 15:15:35: 27000000 INFO @ Tue, 27 Jun 2017 15:15:38: 24000000 INFO @ Tue, 27 Jun 2017 15:15:38: 24000000 INFO @ Tue, 27 Jun 2017 15:15:42: 28000000 INFO @ Tue, 27 Jun 2017 15:15:46: 25000000 INFO @ Tue, 27 Jun 2017 15:15:46: 25000000 INFO @ Tue, 27 Jun 2017 15:15:49: 29000000 INFO @ Tue, 27 Jun 2017 15:15:54: 26000000 INFO @ Tue, 27 Jun 2017 15:15:54: 26000000 INFO @ Tue, 27 Jun 2017 15:15:56: 30000000 INFO @ Tue, 27 Jun 2017 15:16:01: 27000000 INFO @ Tue, 27 Jun 2017 15:16:01: 27000000 INFO @ Tue, 27 Jun 2017 15:16:03: 31000000 INFO @ Tue, 27 Jun 2017 15:16:09: 28000000 INFO @ Tue, 27 Jun 2017 15:16:09: 28000000 INFO @ Tue, 27 Jun 2017 15:16:10: 32000000 INFO @ Tue, 27 Jun 2017 15:16:17: 29000000 INFO @ Tue, 27 Jun 2017 15:16:17: 33000000 INFO @ Tue, 27 Jun 2017 15:16:17: 29000000 INFO @ Tue, 27 Jun 2017 15:16:25: 34000000 INFO @ Tue, 27 Jun 2017 15:16:25: 30000000 INFO @ Tue, 27 Jun 2017 15:16:25: 30000000 INFO @ Tue, 27 Jun 2017 15:16:29: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:16:29: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:16:29: #1 total tags in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:16:29: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:16:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:16:30: #1 tags after filtering in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:16:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:16:30: #1 finished! INFO @ Tue, 27 Jun 2017 15:16:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:16:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:16:32: #2 number of paired peaks: 10 WARNING @ Tue, 27 Jun 2017 15:16:32: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:16:32: Process for pairing-model is terminated! cat: SRX026869.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX026869.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:16:33: 31000000 INFO @ Tue, 27 Jun 2017 15:16:33: 31000000 INFO @ Tue, 27 Jun 2017 15:16:41: 32000000 INFO @ Tue, 27 Jun 2017 15:16:41: 32000000 INFO @ Tue, 27 Jun 2017 15:16:49: 33000000 INFO @ Tue, 27 Jun 2017 15:16:49: 33000000 INFO @ Tue, 27 Jun 2017 15:16:56: 34000000 INFO @ Tue, 27 Jun 2017 15:16:56: 34000000 INFO @ Tue, 27 Jun 2017 15:17:02: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:02: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:02: #1 total tags in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:17:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:02: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:17:02: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:17:02: #1 total tags in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:17:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:17:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:17:02: #1 tags after filtering in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:17:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:02: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:02: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:02: #1 tags after filtering in treatment: 34642938 INFO @ Tue, 27 Jun 2017 15:17:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:17:02: #1 finished! INFO @ Tue, 27 Jun 2017 15:17:02: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:17:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:17:04: #2 number of paired peaks: 10 WARNING @ Tue, 27 Jun 2017 15:17:04: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:04: Process for pairing-model is terminated! cat: SRX026869.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX026869.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:17:05: #2 number of paired peaks: 10 WARNING @ Tue, 27 Jun 2017 15:17:05: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:17:05: Process for pairing-model is terminated! cat: SRX026869.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX026869.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX026869.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。